A click chemistry-based biorthogonal approach for the detection and identification of protein lysine malonylation for osteoarthritis research
Anupama Binoy,
Pandurangan Nanjan,
Kavya Chellamuthu
et al.
Abstract:Lysine malonylation is a post-translational modification where a malonyl group, characterized by a negatively charged carboxylate, is covalently attached to the Ɛ-amino side chain of lysine, influencing protein structure and function. Our laboratory identified Mak upregulation in cartilage under aging and obesity, contributing to osteoarthritis (OA). Current antibody-based detection methods face limitations in identifying Mak targets. Here, we introduce an alkyne-functionalized probe, MA-diyne, which metabolic… Show more
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