A clonal cell line (BME-UV1) as a possible model to study bovine mammary epithelial metabolism: metabolism and cytotoxicity of aflatoxin B1

High expression of the mRNA of cytochrome P450 and phase II enzymes in the lung and kidney tissues of cattle

“…Takiguchi et al (2010) also observed high CYP1A1 mRNA and protein expression in the tongue of the rat. The relatively high expression of CYP1A-like mRNA in the mammary glands may be correlated to the occurrence of a remarkable rate of aflatoxin B1 hydroxylation to aflatoxin M1 in a clonal cell line established from primary bovine mammary epithelial cells as mentioned by Caruso et al (2009). Moreover, it was reported that the mRNA and protein expression of CYP1A1 and CYP1A2 were significantly induced by the cow milkbased formula in HepG2 cells (Xu et al, 2005).…”

Aflatoxin B1 and aflatoxin M1 induced cytotoxicity and DNA damage in differentiated and undifferentiated Caco-2 cells

“…In vitro, AFM1 causes acute toxicity on human B lymphoblastoid MCL-5 cells and cHol cells, which express or do not express human cytochrome P450 enzymes, respectively (Neal et al, 1998). A previous finding documented that AFB1 reduced bovine mammary stabilized epithelial cell viability with a concentration range of 4e16 mM (Caruso et al, 2009), and the probable underlying mechanism might involve the formation of DNA adduct AFB1-8,9-exo-epoxide (AFBO) (Bedard and Massey, 2006;Hamid et al, 2013), which causes DNA damage and is also capable of inducing aflatoxicosis when it binds to protein amino acids in vitro (Hamid et al, 2013).…”

Plenary lectures, Keynotes and Oral Communications

“…Little is known about the expression of such enzymes in the bovine mammary gland. Interestingly, the (CYPmediated) bioactivation of aflatoxin B1 to aflatoxin M1 coupled to a marked cytotoxicity has been recently demonstrated in a bovine mammary epithelial cell line (BME-UV) (Caruso, 2009). In this report we characterized for the first time the basal expression of AhR-target genes in such cells and their modulation by selected agonists.…”