A Closed Tube Loop-Mediated Isothermal Amplification Assay for Identification of Brucella Species in Bull Semen

PCR Based Molecular Diagnostic Assays for Brucellosis: A Review

“…Detection of amplification product can be determined via photometry for turbidity caused by an increasing quantity of magnesium pyrophosphate precipitate in solution as a by product of amplification (Mori et al, 2001). After the discovery of LAMP assay by Notomi et al, 2000 a lot of work have been done on developing LAMP assays (Ohtsuki et al, 2008;Lin et al, 2011;Pan et al, 2011;Song et al, 2012;Perez-Sancho et al, 2013;Chen et al, 2013;Soleimani et al, 2013;Karthik et al, 2014;Marcos et al, 2015;Prusty et al, 2016a, Prusty et al, 2016band Amenov et al, 2017 for identification of Brucella spp. in infected animals as well as humans.…”

“…The target genes for development of these different assays were similar to those which were used in PCR assays. Therefore, likewise PCR these assays targeted bscp-31 (Ohtsuki et al, 2008;Marcos et al, 2015 andPrusty et al, 2016a), omp 25 (Lin et al, 2011;Pan et al, 2011;Song et al, 2012;Chen et al, 2013;Soleimani et al, 2013 andPrusty et al, 2016b) and IS711 (Perez-Sancho et al, 2013 andAmenov et al, 2017) for identification of Brucella at the genus-specific level while BruAb_0168 gene was used (Karthik et al, 2014 andKang et al, 2015) for identification of Brucella abortus. These assays were found to be highly sensitive, specific and easy to perform and can be used in field conditions for detection of Brucella at genus level as well as at species level in clinical samples.…”