A colorimetric and electrochemical immunosensor for point-of-care detection of enterovirus 71

Hou, Yuan-Hui; Wang, Jiajia; Jiang, Yongzhong; Lü, Cheng; Li, Xia; Hong, Shao-Li; Lin, Miao; Lin, Yi; Zhang, Zhiling; Pang, Dai‐Wen

doi:10.1016/j.bios.2017.07.035

Cited by 106 publications

(45 citation statements)

References 37 publications

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“…For example, electrochemical-colorimetric (EC-C) biosensing combines an electrochemical method and a colorimetric, fluorescent, or luminescent detection method. The electrode detects the presence of a target species, while the colorimetric transduction pathway enables quantification of the products associated with the reaction between the target and an active species (Hou et al 2018). For example, Hou et al used an EC-C approach based on a monoclonal antibody-functionalized AuNP-modified ITO electrode and dual-labeled magnetic beads for the detection of human enterovirus 71 (Hou et al 2018).…”

Section: Integration Of Complementary Transduction Elementsmentioning

confidence: 99%

“…The electrode detects the presence of a target species, while the colorimetric transduction pathway enables quantification of the products associated with the reaction between the target and an active species (Hou et al 2018). For example, Hou et al used an EC-C approach based on a monoclonal antibody-functionalized AuNP-modified ITO electrode and dual-labeled magnetic beads for the detection of human enterovirus 71 (Hou et al 2018). In that study, antibody-and horseradish peroxidase (HRP)-labeled magnetic nanobeads were introduced as a secondary binding step following exposure of the electrode to enterovirus-containing samples.…”

Section: Integration Of Complementary Transduction Elementsmentioning

confidence: 99%

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Electrochemical biosensors for pathogen detection

Cesewski

Johnson

2020

Biosensors and Bioelectronics

638

358

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A B S T R A C TRecent advances in electrochemical biosensors for pathogen detection are reviewed. Electrochemical biosensors for pathogen detection are broadly reviewed in terms of transduction elements, biorecognition elements, electrochemical techniques, and biosensor performance. Transduction elements are discussed in terms of electrode material and form factor. Biorecognition elements for pathogen detection, including antibodies, aptamers, and imprinted polymers, are discussed in terms of availability, production, and immobilization approach. Emerging areas of electrochemical biosensor design are reviewed, including electrode modification and transducer integration. Measurement formats for pathogen detection are classified in terms of sample preparation and secondary binding steps. Applications of electrochemical biosensors for the detection of pathogens in food and water safety, medical diagnostics, environmental monitoring, and bio-threat applications are highlighted. Future directions and challenges of electrochemical biosensors for pathogen detection are discussed, including wearable and conformal biosensors, detection of plant pathogens, multiplexed detection, reusable biosensors for process monitoring applications, and low-cost, disposable biosensors.

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Section: Integration Of Complementary Transduction Elementsmentioning

confidence: 99%

Section: Integration Of Complementary Transduction Elementsmentioning

confidence: 99%

Electrochemical biosensors for pathogen detection

Cesewski

Johnson

2020

Biosensors and Bioelectronics

638

358

View full text Add to dashboard Cite

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“…In our previous work, we have developed some biomedical applications based on magnetic beads on the microfluidic chip [4,22,[27][28][29][30][31], and also dedicating ourselves to the construction of microfluidic devices [32][33][34]. On this previous basis, a magnetism and size mediated multiphysics field microfluidic chip was constructed for simultaneous and automated detection of multiple influenza HA (Scheme 1).…”

Section: Introductionmentioning

confidence: 99%

Simultaneous and automated detection of influenza A virus hemagglutinin H7 and H9 based on magnetism and size mediated microfluidic chip

Wang

Zhang

et al. 2020

Sensors and Actuators B: Chemical

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A B S T R A C TInfluenza viruses with multiple subtypes have highly virulent in humans, of which influenza hemagglutinin (HA) is the major viral surface antigen. Simultaneous and automated detection of multiple influenza HA are of great importance for early-stage diagnosis and operator protection. Herein, a magnetism and size mediated microfluidic platform was developed for point-of-care detection of multiple influenza HA. With multiplex microvalves and computer program control, the detection process showed high automation which had a great potential for avoiding the high-risk virus exposure to the operator. Taking advantage of magnetism and size mediated multiple physical fields, multiple influenza HA could be simultaneous separation and detection depended on different-size magnetic beads. Using high-luminance quantum dots as reporter, this assay achieved high sensitivity with a detection limit of 3.4 ng/mL for H7N9 HA and 4.5 ng/mL for H9N2 HA, and showed excellent specificity, anti-interference ability and good reproducibility. These results indicate that this method may propose new avenues for early detection of multiple influenza subtypes.

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“…Enterovirus 71 (EV71) is a non-enveloped, positive-sense, single-stranded RNA virus, which is one of the Picornaviridae family [1,2]. This virus was originally isolated and identified from the feces of an infant suffering from encephalitis in 1969 in California, USA [3].…”

Section: Introductionmentioning

confidence: 99%

Functionalized selenium nanoparticles enhance the anti-EV71 activity of oseltamivir in human astrocytoma cell model

Zhong

Xia

Hua

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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Enterovirus 71 (EV71) which commonly caused the hand-foot-mouth disease (HFMD) has become one of public health challenges worldwide. However, no effective vaccines or drugs for this disease has been developed. Thus, there is an urgent need to find a new strategy for treating the EV71 infection. Oseltamivir (OT) is an effective antiviral agent, but continuous use of oseltamivir leads to a diminished therapeutic effect in the clinic. In order to improve the antiviral activity of oseltamivir, oseltamivir was loaded onto surfaces of selenium nanoparticles (SeNPs) to fabricate a functionalized antiviral nanoparticles SeNPs@OT. The size of SeNPs@OT was tested by TEM and dynamic light scattering. The chemical structure and elemental composition of SeNPs@OT were analyzed by FT-IR and EDX, respectively. SeNPs@OT exhibited good stability and effective drug release in serum and PBS. SeNPs@OT efficiently entered into human astrocyte U251 cells (host cells) via clathrin-associated endocytosis and inhibited EV71 proliferation, which could protect EV71-infected U251 cells from apoptosis through mitochondrial pathway. Furthermore, SeNPs@OT inhibited EV71 activity probably by reducing the generation of reactive oxygen species in EV71-infected U251 cells. Interestingly, SeNPs obviously enhanced antiviral activity of oseltamivir in the anti-EV71 cell model. Taken together, SeNPs@OT is a promising antiviral drug candidate for EV71 infection.

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A colorimetric and electrochemical immunosensor for point-of-care detection of enterovirus 71

Cited by 106 publications

References 37 publications

Electrochemical biosensors for pathogen detection

Electrochemical biosensors for pathogen detection

Simultaneous and automated detection of influenza A virus hemagglutinin H7 and H9 based on magnetism and size mediated microfluidic chip

Functionalized selenium nanoparticles enhance the anti-EV71 activity of oseltamivir in human astrocytoma cell model

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