A colorimetric aptasensor for the simple and rapid detection of human papillomavirus type 16 L1 proteins

Zhu, Li; Yu, Zhao; Yao, Shangchen; Xu, Ming; Liang, Yin; Zhai, Xihai; Teng, Xu

doi:10.1039/d1an00251a

Cited by 15 publications

(8 citation statements)

References 38 publications

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“…Even in the presence of ppb levels of protein, if salt was added, the GNP and aptamer aggregated. HPV16 L1 protein levels as low as 9.6 ng/mL could be detected [ 105 ].…”

Section: Application Of Nanobiosensors In Viral Diagnosticsmentioning

confidence: 99%

Impact of nanotechnology on conventional and artificial intelligence-based biosensing strategies for the detection of viruses

et al. 2023

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Recent years have witnessed the emergence of several viruses and other pathogens. Some of these infectious diseases have spread globally, resulting in pandemics. Although biosensors of various types have been utilized for virus detection, their limited sensitivity remains an issue. Therefore, the development of better diagnostic tools that facilitate the more efficient detection of viruses and other pathogens has become important. Nanotechnology has been recognized as a powerful tool for the detection of viruses, and it is expected to change the landscape of virus detection and analysis. Recently, nanomaterials have gained enormous attention for their value in improving biosensor performance owing to their high surface-to-volume ratio and quantum size effects. This article reviews the impact of nanotechnology on the design, development, and performance of sensors for the detection of viruses. Special attention has been paid to nanoscale materials, various types of nanobiosensors, the internet of medical things, and artificial intelligence-based viral diagnostic techniques.

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“…Even in the presence of ppb levels of protein, if salt was added, the GNP and aptamer aggregated. HPV16 L1 protein levels as low as 9.6 ng/mL could be detected [ 105 ].…”

Section: Application Of Nanobiosensors In Viral Diagnosticsmentioning

confidence: 99%

Impact of nanotechnology on conventional and artificial intelligence-based biosensing strategies for the detection of viruses

et al. 2023

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“…Such an easy, simple method based on naked-eye colorimetric detection opens up many other possibilities as reflected by several similar examples published, e.g., AuNPs coated with unfunctionalized polyadenine aptamers to detect prostate specific antigen in serum [ 93 ]; AuNPs coated with unfunctionalized truncated forms of aptamers to detect bisphenol A in food and water [ 94 ]; AuNPs coated with unfunctionalized aptamers to detect chloramphenicol and tetracycline in food [ 95 ]; AuNPs coated with unfunctionalized aptamers for detection of Bacillus carboniphilus on bacterial biofilms [ 96 ]; AuNPs coated with unfunctionalized RNA aptamers for detection of human papillomavirus type 16 L1 in clinical and vaccine samples [ 97 ]; and AuNPs coated with unfunctionalized aptamers to detect acetamiprid in food and water [ 98 ].…”

Section: Biosensors Based On Hybrid Biomolecules-nanomaterials Compos...mentioning

confidence: 99%

Bio-Tailored Sensing at the Nanoscale: Biochemical Aspects and Applications

Fata

Gabriele²,

Angelucci³

et al. 2023

Sensors

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The demonstration of the first enzyme-based electrode to detect glucose, published in 1967 by S. J. Updike and G. P. Hicks, kicked off huge efforts in building sensors where biomolecules are exploited as native or modified to achieve new or improved sensing performances. In this growing area, bionanotechnology has become prominent in demonstrating how nanomaterials can be tailored into responsive nanostructures using biomolecules and integrated into sensors to detect different analytes, e.g., biomarkers, antibiotics, toxins and organic compounds as well as whole cells and microorganisms with very high sensitivity. Accounting for the natural affinity between biomolecules and almost every type of nanomaterials and taking advantage of well-known crosslinking strategies to stabilize the resulting hybrid nanostructures, biosensors with broad applications and with unprecedented low detection limits have been realized. This review depicts a comprehensive collection of the most recent biochemical and biophysical strategies for building hybrid devices based on bioconjugated nanomaterials and their applications in label-free detection for diagnostics, food and environmental analysis.

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“…In recent years, aptamer-based colorimetric sensing methods have emerged as alternatives to detect various target molecules [8][9][10][11][12][13], because of their simplicity, low cost, high sensitivity, and specific detection without the requirement of complex instrumentation [14]. In this assay, aptamers (single-stranded DNA or RNA oligonucleotides) are used as recognition elements owing to their ability to selectively bind their target molecules, which helps improve the sensor specificity [15][16][17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

“…In colorimetric sensors, gold nanoparticles (AuNPs) are commonly used as colorimetric reporters because of their high extinction coefficients and size-dependent optical properties [8,22]. The color of the dispersed AuNP solution is wine-red.…”

Section: Introductionmentioning

confidence: 99%

“…In addition, AuNPs have been used in combination with other nanomaterials (e.g., reduced graphene oxide) or conjugated with aptamers to improve the performance of the sensors or to enhance the detection sensitivity [25][26][27]. Moreover, AuNPs can adsorb unfolded aptamers through electrostatic and noncovalent interactions, which protects AuNPs from salt-induced aggregation by enhancing the electrostatic repulsion between nanoparticles [8,22,28]. However, when aptamers bind to their target molecules, their conformation changes.…”

Section: Introductionmentioning

confidence: 99%

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A Simple and Ultrasensitive Colorimetric Biosensor for Anatoxin-a Based on Aptamer and Gold Nanoparticles

Nguyen

Jang

2021

Micromachines

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Here, we designed a simple, rapid, and ultrasensitive colorimetric aptasensor for detecting anatoxin-a (ATX-a). The sensor employs a DNA aptamer as the sensing element and gold nanoparticles (AuNPs) as probes. Adsorption of the aptamer onto the AuNP surface can protect AuNPs from aggregation in NaCl solution, thus maintaining their dispersion state. In the presence of ATX-a, the specific binding of the aptamer with ATX-a results in a conformational change in the aptamer, which facilitates AuNP aggregation and, consequently, a color change of AuNPs from red to blue in NaCl solution. This color variation is directly associated with ATX-a concentration and can be easily measured using a UV/Vis spectrophotometer. The absorbance variation is linearly proportional to ATX-a concentration across the concentration range of 10 pM to 200 nM, with a detection limit of 4.45 pM and high selectivity against other interferents. This strategy was successfully applied to the detection of ATX-a in lake water samples. Thus, the present aptasensor is a promising alternative method for the rapid detection of ATX-a in the environment.

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A colorimetric aptasensor for the simple and rapid detection of human papillomavirus type 16 L1 proteins

Abstract: In this study, a novel colorimetric aptasensor was developed for the rapid detection and visual screening of HPV16 L1 proteins by using the gold nanoparticles (AuNPs) and an RNA aptamer...

Cited by 15 publications

References 38 publications

Impact of nanotechnology on conventional and artificial intelligence-based biosensing strategies for the detection of viruses

Impact of nanotechnology on conventional and artificial intelligence-based biosensing strategies for the detection of viruses

Bio-Tailored Sensing at the Nanoscale: Biochemical Aspects and Applications

A Simple and Ultrasensitive Colorimetric Biosensor for Anatoxin-a Based on Aptamer and Gold Nanoparticles

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