A column-switching LC/MS/ESI method for detecting tetrodotoxin and Aconitum alkaloids in serum

Hayashida, Makiko; Hayakawa, Hideyuki; Wada, Kentaro; Yamada, Takeshi; Nihira, Makoto; Ohno, Youkichi

doi:10.1016/s1344-6223(02)00076-7

Cited by 29 publications

(22 citation statements)

References 3 publications

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“…Hayaschida and co-workers developed a LC-MS method which utilised electrospray ionisation and detection in the selected ion monitoring mode for the determination of several Aconitum alkaloids in serum samples [30].…”

Section: Introductionmentioning

confidence: 99%

Determination of aconitine in body fluids by LC-MS-MS

et al. 2004

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A very sensitive and specific method was developed for the determination of aconitine, the main toxic alkaloid from plants of the genus Aconitum L., in biological samples. The method comprised solid-phase extraction using mixed-mode Cs cation exchange columns followed by liquid chromatography-tandem mass spectrometry (LC-MS-MS). Chromatographic separation was achieved with a RP8 column. Detection of aconitine was achieved using electrospray in the positive ionisation mode and quantification was performed using multiple reaction monitoring with m/z 646.4 as precursor ion, i.e. [M+H]+ of aconitine and m/z 586.5, m/z 526.4 and m/z 368.4 as product ions after collision-induced dissociation. The method was fully validated for the analysis of blood samples: the limit of detection and the limit of quantitation were 0.1 ng/g and 0.5 ng/g, respectively. Within the linear calibration range of 0.5-25 ng/g, analytical recovery was 79.9%. In two fatal cases with suspected aconite intoxication, aconitine could be detected in blood samples at concentrations of 10.0 and 12.1 ng/g. In one case, aconitine could also be detected in the stomach content (3 ng/g) and in the other in the urine (180 ng/ml).

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Section: Introductionmentioning

confidence: 99%

Determination of aconitine in body fluids by LC-MS-MS

et al. 2004

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“…Lethal potency of TTX is normally expressed using mouse unit (MU); one MU is approximately 0.22 g TTX [25]. The sensitivity of this assay was estimated at 0.00023 MU, better than those of indirect competitive inhibition EIA (0.1 ng/mL or ∼0.00041 MU) [23], electrophoresis and TLC (2 g) [16], LC with fluorescent detectors (3.0, 4.4, 10.0 ng) [16,19,20], LC-MS assay (0.2 ng) [18], LC-MS/MS (0.09 ng) [4] and TLC/fast atom bombardment MS (0.1 g) [30]. Nevertheless, by using larger quantities of starting material and HOAc together with commercialized cartridges, Horie et al [25] reported similar on-column assay sensitivity as used in our method.…”

Section: Resultsmentioning

confidence: 99%

“…However, this assay and other techniques such as thin layer chromatography (TLC), electrophoresis [7,16,17], liquid chromatography (LC) [10,[16][17][18][19][20], spectrophotometry [7,12], enzyme immunoassays (EIA) [21][22][23] poses ethical concern, is not specific and lacked sensitivity at low concentration or of poor precision. Mass spectrometry (MS) coupled with LC or GC is a sensitive technique for identification of TTX.…”

Section: Introductionmentioning

confidence: 99%

Screening of tetrodotoxin in puffers using gas chromatography–mass spectrometry

Man

Noor

Harn

et al. 2010

Journal of Chromatography A

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“…Several methods including high-performance liquid chromatography (HPLC) with UV detection and mass spectrometry (MS) were developed for the determination of Aconitum alkaloids including aconitine, mesaconitine and hypaconitine etc. in blood or urine [4][5][6][7]. Gas chromatography (GC) with MS detection that needed tedious derivatization has also been reported [8].…”

Section: Introductionmentioning

confidence: 99%