A Combined Proteomic and Transcriptomic Analysis on Sulfur Metabolism Pathways of Arabidopsis thaliana under Simulated Acid Rain

Liu, Tingwu; Chen, Juan A.; Wang, Wenhua; Simón, Martín; Hu, Wenjun; Zheng, Hai-Lei

doi:10.1371/journal.pone.0090120

Cited by 16 publications

(21 citation statements)

References 54 publications

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“…These proteins included sulfur dioxygenase, sulfite oxidase, adenylyl-sulfate reductase, and inositol polyphosphate 1phosphatase. Sulfur is an essential mineral element involved in fundamental processes such as electron transport, structure, and regulation (Flávia, Capaldi Priscila, Gratão André, Reis Lima, & Azevedo, 2015 (Bick & Leustek, 1998;Liu et al, 2014). The results regarding sulfur metabolism are consistent with the improvement of the AsA-glutathione cycle and Cys and Met metabolism (described in detail in the subsequent section on amino acid metabolism) and indicated that AMF inoculation significantly improved sulfur metabolism to provide substrates for ROS and amino acid metabolism and thus enhanced the alkali tolerance of the P. tenuiflora seedlings.…”

Section: Sulphur Metabolismsupporting

confidence: 72%

“…It is taken up as sulfate and then assimilated into sulfur‐containing compounds. The compounds are crucial for plant survival under abiotic stress, and they include elemental sulphur, hydrogen sulfide, glutathione, phytochelatins, sulfur‐rich proteins (containing high levels of amino acids such as cysteine [Cys] and methionine [Met]), and several secondary metabolites (Bick & Leustek, ; Liu et al, ). The results regarding sulfur metabolism are consistent with the improvement of the AsA‐glutathione cycle and Cys and Met metabolism (described in detail in the subsequent section on amino acid metabolism) and indicated that AMF inoculation significantly improved sulfur metabolism to provide substrates for ROS and amino acid metabolism and thus enhanced the alkali tolerance of the P. tenuiflora seedlings.…”

Section: Discussionmentioning

confidence: 99%

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Isobaric tags for relative and absolute quantification‐based proteomic analysis of Puccinellia tenuiflora inoculated with arbuscular mycorrhizal fungi reveal stress response mechanisms in alkali‐degraded soil

et al. 2019

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Alkali soil is a major abiotic constraint that limits plant distribution and yield in the northeast of China. Puccinellia tenuiflora is considered the most promising grass species for salt‐alkali grassland restoration. However, there is little information on the molecular mechanisms underlying how arbuscular mycorrhizal fungi (AMF) enhances P. tenuiflora stress responses in alkali‐degraded soil. In this study, AMF colonization, growth, photosynthetic pigments, and inorganic ion contents were measured. Isobaric tags for relative and absolute quantification‐based quantitative proteomic technology were employed to identify the differentially abundant proteins in P. tenuiflora seedlings with or without AMF under alkalinity stress. The results showed that AMF colonization increased seedling biomass, photosynthetic pigment contents, and K+/Na+ ratio under alkalinity stress. Moreover, a total of 598 proteins were significantly differentially regulated in P. tenuiflora seedlings after AMF inoculation under alkalinity stress compared with under alkalinity stress alone. The results showed that AMF inoculation significantly improved protein synthesis, reactive oxygen species scavenging, and nitrogen metabolism to promote the biosynthesis of osmotic substances in response to alkalinity stress. In addition, P. tenuiflora seedlings produced more energy to compensate for the energy loss caused by alkalinity stress after AMF inoculation. In conclusion, these findings provide new insights into the physiological mechanisms of the response to alkali‐degraded soil in P. tenuiflora seedlings with AMF and also clarify the role of AMF in the molecular regulation network of P. tenuiflora under alkalinity stress.

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Section: Sulphur Metabolismsupporting

confidence: 72%

Section: Discussionmentioning

confidence: 99%

Isobaric tags for relative and absolute quantification‐based proteomic analysis of Puccinellia tenuiflora inoculated with arbuscular mycorrhizal fungi reveal stress response mechanisms in alkali‐degraded soil

et al. 2019

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“…Their results [ 18 ] of RNA-sequence and qRT-PCR suggested that the regulatory genes of different secondary metabolites were downregulated by AR treatment in relation to control vs AR-stressed tomato plant ( Table 1 ). Similar to these results, Liu et al [ 127 ] observed that AR treatment changed the expression pattern of differential genes and also the genes associated with secondary metabolites in plants. To the contrary, the use of melatonin in AR-stressed plants caused upregulation of secondary gene expression in control vs melatonin-treated AR-stressed plants, and AR-stressed plants vs melatonin-treated AR-stressed plants ( Table 1 ) [ 18 ].…”

Section: Role Of Melatonin In Plants Under Acid Rain Stresssupporting

confidence: 72%

Physiological and Molecular Responses to Acid Rain Stress in Plants and the Impact of Melatonin, Glutathione and Silicon in the Amendment of Plant Acid Rain Stress

et al. 2021

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Air pollution has been a long-term problem, especially in urban areas, that eventually accelerates the formation of acid rain (AR), but recently it has emerged as a serious environmental issue worldwide owing to industrial and economic growth, and it is also considered a major abiotic stress to agriculture. Evidence showed that AR exerts harmful effects in plants, especially on growth, photosynthetic activities, antioxidant activities and molecular changes. Effectiveness of several bio-regulators has been tested so far to arbitrate various physiological, biochemical and molecular processes in plants under different diverse sorts of environmental stresses. In the current review, we showed that silicon (tetravalent metalloid and semi-conductor), glutathione (free thiol tripeptide) and melatonin (an indoleamine low molecular weight molecule) act as influential growth regulators, bio-stimulators and antioxidants, which improve plant growth potential, photosynthesis spontaneity, redox-balance and the antioxidant defense system through quenching of reactive oxygen species (ROS) directly and/or indirectly under AR stress conditions. However, earlier research findings, together with current progresses, would facilitate the future research advancements as well as the adoption of new approaches in attenuating the consequence of AR stress on crops, and might have prospective repercussions in escalating crop farming where AR is a restraining factor.

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“…Although only a subset of the phloem RNA fraction was analysed, results regarding the regulatory function of miRNA-395 were substantiated in further studies employing Arabidopsis (Kawashima et al, 2009, 2011). Sulphate metabolism in response to acid rain conditions has been investigated, with high inputs of S under low pH conditions (Liu et al , 2014). Acid rain is an ecological and a health problem in many countries due to combustion of fossil fuels releasing SO 2 .…”

Section: Transcriptomics Of Arabidopsis With Respect To Sulphur Metabmentioning

confidence: 99%

Sulphur systems biology—making sense of omics data

Watanabe

Hoefgen

2019

Journal of Experimental Botany

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Systems biology approaches have been applied over the last two decades to study plant sulphur metabolism. These ‘sulphur-omics’ approaches have been developed in parallel with the advancing field of systems biology, which is characterized by permanent improvements of high-throughput methods to obtain system-wide data. The aim is to obtain a holistic view of sulphur metabolism and to generate models that allow predictions of metabolic and physiological responses. Besides known sulphur-responsive genes derived from previous studies, numerous genes have been identified in transcriptomics studies. This has not only increased our knowledge of sulphur metabolism but has also revealed links between metabolic processes, thus indicating a previously unexpected complex interconnectivity. The identification of response and control networks has been supported through metabolomics and proteomics studies. Due to the complex interlacing nature of biological processes, experimental validation using targeted or systems approaches is ongoing. There is still room for improvement in integrating the findings from studies of metabolomes, proteomes, and metabolic fluxes into a single unifying concept and to generate consistent models. We therefore suggest a joint effort of the sulphur research community to standardize data acquisition. Furthermore, focusing on a few different model plant systems would help overcome the problem of fragmented data, and would allow us to provide a standard data set against which future experiments can be designed and compared.

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A Combined Proteomic and Transcriptomic Analysis on Sulfur Metabolism Pathways of Arabidopsis thaliana under Simulated Acid Rain

Cited by 16 publications

References 54 publications

Isobaric tags for relative and absolute quantification‐based proteomic analysis of Puccinellia tenuiflora inoculated with arbuscular mycorrhizal fungi reveal stress response mechanisms in alkali‐degraded soil

Isobaric tags for relative and absolute quantification‐based proteomic analysis of Puccinellia tenuiflora inoculated with arbuscular mycorrhizal fungi reveal stress response mechanisms in alkali‐degraded soil

Physiological and Molecular Responses to Acid Rain Stress in Plants and the Impact of Melatonin, Glutathione and Silicon in the Amendment of Plant Acid Rain Stress

Sulphur systems biology—making sense of omics data

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