A community-built calibration system: The case study of quantification of metabolites in grape juice by qNMR spectroscopy

Musio, Biagia; Ragone, Rosa; Todisco, Stefano; Rizzuti, Antonino; Latronico, Mario; Mastrorilli, Piero; Pontrelli, Stefania; Intini, Nicola; Scapicchio, P.; Triggiani, Maurizio; Noia, Tommaso Di; Acquotti, Domenico; Airoldi, Cristina; Assfalg, Michael; Bateman, Lorraine M.; Benevelli, Francesca; Bertelli, Davide; Bertocchi, Fabio; Bieliauskas, Aurimas; Borioni, Anna; Caligiani, Augusta; Callone, Emanuela; Čamra, Ales; Marincola, Flaminia Cesare; Chalasani, Dinesh; Consonni, Roberto; Dambruoso, Paolo; Davalli, Silvia; Taylor, DeMar; Diehl, Bernd; Donarski, James; Gil, Ana M.; Gobetto, Roberto; Goldoni, Luca; Hamon, Erwann; Harwood, John S.; Kobrlová, Andrea; Longobardi, Francesco; Luisi, Renzo; Mallamace, Domenico; Mammi, Stefano; Martin-Biran, Magali; Mazzei, Pierluigi; Milone, Salvatore; Vilchez, D. M.; Mulder, Roger J.; Napoli, Claudia Di; Ragno, Daniele; Randazzo, Antonio; Rossi, Maria Cecilia; Rotondo, Archimede; Šačkus, Algirdas; Barajas, Elena Sáez; Schievano, Elisabetta; Bhavaraju, Sitaram; Stevanato, Livio; Takis, Panteleimon G.; Teipel, Jan; Thomas, Freddy; Torregiani, Elisabetta; Valensin, Daniela; Veronesi, Marina; Warren, John; Wist, Julien; Zailer-Hafer, Elina; Zuccaccia, Cristiano; Gallo, Vito

doi:10.1016/j.talanta.2020.120855

Cited by 16 publications

(7 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In recent years, magnetic resonance spectroscopy (NMR) technology has gained increasing prominence in the elds of pharmaceuticals, chemicals and botanical research. [13][14][15] NMR offers signicant potential for rapid and accurate qualication and quantication of complex samples. Quantitative nuclear magnetic resonance spectroscopy (qNMR) has been successfully applied to the analysis of polyphenol extracts.…”

Section: Introductionmentioning

confidence: 99%

“…The results indicated no signicant difference between the two methods, but qNMR analysis exhibited higher precision. In principle, NMR can detect any component containing one or more atoms with a non-zero magnetic moment (such as 1 H, 13 C, 14 N, 15 N and 31 P). Moreover, qNMR does not require standard samples for establishing calibration curves during determination.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Determination of total phenol and six polyphenolic components in the polyphenol extract of Cinnamomi cortex by quantitative nuclear magnetic resonance spectroscopy

Shi,

Zheng,

Guo

et al. 2023

Anal. Methods

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Determination of total phenol and six polyphenolic components in the polyphenol extract of Cinnamomi cortex by quantitative nuclear magnetic resonance spectroscopy

Shi,

Zheng,

Guo

et al. 2023

Anal. Methods

View full text Add to dashboard Cite

show abstract

“…Progress has been made in our laboratory to employ the non-targeted NMR approach [23][24][25][26][27] to reveal Xf infection at different levels of severity in cv. Cellina di Nardò olive trees.…”

Section: Introductionmentioning

confidence: 99%

Non-Targeted Spectranomics for the Early Detection of Xylella fastidiosa Infection in Asymptomatic Olive Trees, cv. Cellina di Nardò

Ahmed,

Musio,

Todisco

et al. 2023

Molecules

Self Cite

View full text Add to dashboard Cite

Olive quick decline syndrome (OQDS) is a disease that has been seriously affecting olive trees in southern Italy since around 2009. During the disease, caused by Xylella fastidiosa subsp. pauca sequence type ST53 (Xf), the flow of water and nutrients within the trees is significantly compromised. Initially, infected trees may not show any symptoms, making early detection challenging. In this study, young artificially infected plants of the susceptible cultivar Cellina di Nardò were grown in a controlled environment and co-inoculated with additional xylem-inhabiting fungi. Asymptomatic leaves of olive plants at an early stage of infection were collected and analyzed using nuclear magnetic resonance (NMR), hyperspectral reflectance (HSR), and chemometrics. The application of a spectranomic approach contributed to shedding light on the relationship between the presence of specific hydrosoluble metabolites and the optical properties of both asymptomatic Xf-infected and non-infected olive leaves. Significant correlations between wavebands located in the range of 530–560 nm and 1380–1470 nm, and the following metabolites were found to be indicative of Xf infection: malic acid, fructose, sucrose, oleuropein derivatives, and formic acid. This information is the key to the development of HSR-based sensors capable of early detection of Xf infections in olive trees.

show abstract

“…Over the last decades, the use of an NMR-based analytical approach for food quality and authentication has significantly increased [4][5][6]. The distinctive ability of NMR spectroscopy compared to other analytical techniques is that of providing reproducible spectral data in terms of relative signal intensity, regardless of the spectrometer features [7,8]. Recently, our research group contributed to the harmonization of NMR data production by differently configured spectrometers [9,10].…”

Section: Introductionmentioning

confidence: 99%

Non-Targeted NMR Method to Assess the Authenticity of Saffron and Trace the Agronomic Practices Applied for Its Production

et al. 2022

Self Cite

View full text Add to dashboard Cite

The development of analytical methods aimed at tracing agri-food products and assessing their authenticity is essential to protect food commercial value and human health. An NMR-based non-targeted method is applied here to establish the authenticity of saffron samples. Specifically, 40 authentic saffron samples were compared with 18 samples intentionally adulterated by using turmeric and safflower at three different concentration levels, i.e., 5, 10, and 20 wt%. Statistical processing of NMR data furnished useful information about the main biomarkers contained in aqueous and dimethyl sulfoxide extracts, which are indicative of the presence of adulterants within the analyzed matrix. Furthermore, a discrimination model was developed capable of revealing the type of agronomic practice adopted during the production of this precious spice, distinguishing between organic and conventional cultivation. The main objective of this work was to provide the scientific community involved in the quality control of agri-food products with an analytical methodology able to extract useful information quickly and reliably for traceability and authenticity purposes. The proposed methodology turned out to be sensitive to minor variations in the metabolic composition of saffron that occur in the presence of the two adulterants studied. Both adulterants can be detected in aqueous extracts at a concentration of 5 wt%. A lower limit of detection was observed for safflower contained in organic extracts in which case the lowest detectable concentration was 20%.

show abstract

A community-built calibration system: The case study of quantification of metabolites in grape juice by qNMR spectroscopy

Cited by 16 publications

References 22 publications

Determination of total phenol and six polyphenolic components in the polyphenol extract of Cinnamomi cortex by quantitative nuclear magnetic resonance spectroscopy

Determination of total phenol and six polyphenolic components in the polyphenol extract of Cinnamomi cortex by quantitative nuclear magnetic resonance spectroscopy

Non-Targeted Spectranomics for the Early Detection of Xylella fastidiosa Infection in Asymptomatic Olive Trees, cv. Cellina di Nardò

Non-Targeted NMR Method to Assess the Authenticity of Saffron and Trace the Agronomic Practices Applied for Its Production

Contact Info

Product

Resources

About