A Compact Control System to Enable Automated Operation of Microfluidic Bioanalytical Assays

Gonzalez-Suarez, Alan M.; Long, Alexander; Huang, XuHai; Revzin, Alexander

doi:10.3390/bios12121160

Cited by 2 publications

(2 citation statements)

References 25 publications

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“…Biosensors employ portable instruments to detect specific biomarkers in bodily fluids, providing a simple and efficient diagnostic approach ( Senf, Yeo & Kim, 2020 ). On the other hand, microfluidic devices offer automation and simplification of complex laboratory procedures, making them suitable for implementation in resource-limited settings like sSA ( Gonzalez-Suarez et al, 2022 ).…”

Section: Current and Emerging Diagnostic Methods For Poverty-related ...mentioning

confidence: 99%

Towards the development of cost-effective point-of-care diagnostic tools for poverty-related infectious diseases in sub-Saharan Africa

Ofori,

Twum,

Nkansah Yeboah

et al. 2024

PeerJ

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In this review, we examine the current landscape of point-of-care testing (POCT) diagnostic tools designed for poverty-related infectious diseases (PRIDs) in sub-Saharan Africa (sSA) while delineating key avenues for future advancements. Our analysis encompasses both established and emerging diagnostic methods for PRIDs, addressing the persistent challenges in POCT tool development and deployment, such as cost, accessibility, and reliability. We emphasize recent advancements in POCT diagnostic tools as well as platforms poised to enhance diagnostic testing in sSA. Recognizing the urgency for affordable and widely accessible POCT diagnostic tools to detect PRIDs in sSA, we advocate for a multidisciplinary approach. This approach integrates current and emerging diagnostic methods, explicitly addressing challenges hindering point-of-care (POC) tool development. Furthermore, it recognizes the profound impact of misdiagnosis on public and global health, emphasizing the need for effective tools. To facilitate the successful development and implementation of POCT diagnostic tools in sSA, we propose strategies including the creation of multi-analyte detection POCT tools, the implementation of education and training programs, community engagement initiatives, fostering public-private collaborations, and the establishment of reliable supply chains. Through these concerted efforts, we aim to accelerate the development of POCT in the sSA region, ensuring its effectiveness and accessibility in addressing the diagnostic challenges associated with PRIDs.

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Section: Current and Emerging Diagnostic Methods For Poverty-related ...mentioning

confidence: 99%

Towards the development of cost-effective point-of-care diagnostic tools for poverty-related infectious diseases in sub-Saharan Africa

Ofori,

Twum,

Nkansah Yeboah

et al. 2024

PeerJ

View full text Add to dashboard Cite

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“…Automated microfluidic devices can perform complex multistep assays like an ELISA when they are embedded with micro valve components to provide proper reagent flow control and timing [ 22 ]. Originally, our proprietary lab-on-paper platform used fluidic valves consisting of a stack of hydrophobic and hydrophilic discs [ 7 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

Development of a New Lab-on-Paper Microfluidics Platform Using Bi-Material Cantilever Actuators for ELISA on Paper

et al. 2023

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In this paper, we present a novel and cost-effective lab-on-paper microfluidics platform for performing ELISA autonomously, with no user intervention beyond adding the sample. The platform utilizes two Bi-Material Cantilever Valves placed in a specially designed housing. The integration of these valves in a specific channel network forms a complete fluidic logic circuit for performing ELISA on paper. The housing also incorporates an innovative reagent storage and release mechanism that minimizes variability in the volume of reagents released into the reagent pads. The platform design was optimized to minimize variance in the time of fluid wicking from the reagent pad, using a randomized design of experiment. The platform adheres to the World Health Organization’s ASSURED principles. The optimized design was used to conduct an ELISA for detecting rabbit immunoglobulin G (IgG) in a buffer, with a limit of detection of 2.27 ng/mL and a limit of quantification of 8.33 ng/mL. This represents a 58% improvement over previous ELISA methods for detecting rabbit IgG in buffer using portable microfluidic technology.

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A Compact Control System to Enable Automated Operation of Microfluidic Bioanalytical Assays

Cited by 2 publications

References 25 publications

Towards the development of cost-effective point-of-care diagnostic tools for poverty-related infectious diseases in sub-Saharan Africa

Towards the development of cost-effective point-of-care diagnostic tools for poverty-related infectious diseases in sub-Saharan Africa

Development of a New Lab-on-Paper Microfluidics Platform Using Bi-Material Cantilever Actuators for ELISA on Paper

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