A multi-frequency microstrip antenna loaded with a ring-like structure has been proposed. The radiating patch on the antenna surface consists of three split-ring resonator structures, and the ground plate consists of a bottom metal strip and three ring-shaped metals with regular cuts to form a defective ground structure. The proposed antenna works in six different frequency bands covering 1.10, 1.33, 1.63, 1.97, 2.08, and 2.69 GHz and works entirely when connected to 5G NR (FR1, 0.45–3 GHz), 4GLTE (1.6265–1.6605 GHz), Personal Communication System (1.85–1.99 GHz), Universal Mobile Telecommunications System (1.92–2.176 GHz), WiMAX (2.5–2.69 GHz), and other communications frequency bands. Moreover, such antennas also have stable omnidirectional radiation properties across different operating frequency bands. This antenna meets the needs of portable multi-frequency mobile devices and provides a theoretical approach for the development of multi-frequency antennas.