A Comparative Analysis of the Hybrid Maize (Zea mays L.) Seed Quality in China from 2013 to 2018

et al. 2021

AMB Expr

Self Cite

Crop performance is seriously affected by high salt concentrations in soils. To develop improved seed pre-sowing treatment technologies, it is crucial to improve the salt tolerance of seed germination. Here, we isolated and identified the strain Bacillus sp. MGW9 and developed the seed biostimulant MGW9. The effects of seed biopriming with the seed biostimulant MGW9 in maize (Zea mays L.) under saline conditions were studied. The results show that the strain Bacillus sp. MGW9 has characteristics such as salt tolerance, nitrogen fixation, phosphorus dissolution, and indole-3-acetic acid production. Seed biopriming with the seed biostimulant MGW9 enhanced the performance of maize during seed germination under salinity stress, improving the germination energy, germination percentage, shoot/seedling length, primary root length, shoot/seedling fresh weight, shoot/seedling dry weight, root fresh weight and root dry weight. Seed biostimulant MGW9 biopriming also alleviated the salinity damage to maize by improving the relative water content, chlorophyll content, proline content, soluble sugar content, root activity, and activities of superoxide dismutase, catalase, peroxidase and ascorbate peroxidase, while decreasing the malondialdehyde content. In particular, the field seedling emergence of maize seeds in saline-alkali soil can be improved by biopriming with the seed biostimulant MGW9. Therefore, maize seed biopriming with the seed biostimulant MGW9 could be an effective approach to overcoming the inhibitory effects of salinity stress and promoting seed germination and seedling growth.

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Seed biostimulant Bacillus sp. MGW9 improves the salt tolerance of maize during seed germination

et al. 2021

AMB Expr

Self Cite

“…Pure maize seeds were randomly selected from each sample for the following experiments. i) Thousand-seed weight (TSW) test: the TSW was measured using 500 seeds in each of the three replicates and then converted to thousand seed weight (Li et al 2019a).…”

Section: Identi Cation Of Strain Mgw9mentioning

confidence: 99%

“…The germination energy (GE) and germination percentage (GP) were measured on the 4th and 7th day after the experiment was established. GP is the normal seedling number on the 7th day after seed planting (Li et al 2019a). While counting the GP, 10 seedlings with uniform size were randomly selected to measure six indices, including shoot/seedling length (SL), primary root length (PRL), shoot/seedling fresh weight (SFW), shoot/seedling dry weight (SDW), root fresh weight (RFW) and root dry weight (RDW).…”

Section: Germination and Seedling Growth Testmentioning

confidence: 99%

Seed Biostimulant MGW9 (SB-MGW9) Biopriming Improves Salt Tolerance during Maize Seed Germination

et al. 2021

Preprint

Self Cite

Crop performance is seriously affected by high salt concentrations in soils. To develop more new seed pre-sowing treatment technologies it is crucial to improve the salt tolerance of seed germination. Here we isolated and identified the strain Bacillus sp. MGW9 and developed the seed biostimulant MGW9 (SB-MGW9) by the strain. Effect of seed biopriming with SB-MGW9 in maize (Zea mays L.) under saline condition were studied. The results showed that the Bacillus sp. MGW9 has the characteristics of salt tolerance, nitrogen fixation, phosphorus dissolution, indole-3-acetic acid production and the like. Seed biopriming with SB-MGW9 enhanced the performance of maize during seed germination under salinity stress to improve the germination energy, germination percentage, shoot/seedling length, primary root length, shoot/seedling fresh weight, shoot/seedling dry weight, root fresh weight and root dry weight. SB-MGW9 biopriming also alleviates the salinity damage to maize by improving relative water content, chlorophyll content, proline content, soluble sugar content, root activity, activities of superoxide dismutase, catalase, peroxidase and ascorbate peroxidase, decreasing the malondialdehyde content. Especially, the field seedling emergence of maize seeds in saline-alkali soil can be improved by SB-MGW9 biopriming. Therefore, maize seed biopriming with SB-MGW9 can be an effective approach to resist the inhibitory effects of salinity stress and promote seed germination and seedling growth.

Transcriptomic profiling of the high-vigour maize (Zea mays L.) hybrid variety response to cold and drought stresses during seed germination

Xie

et al. 2021

Sci Rep

Abiotic stresses, including cold and drought, negatively affect maize (Zea mays L.) seed field emergence and later yield and quality. In order to reveal the molecular mechanism of maize seed resistance to abiotic stress at seed germination, the global transcriptome of high- vigour variety Zhongdi175 exposed to cold- and drought- stress was analyzed by RNA-seq. In the comparison between the control and different stressed sample, 12,299 differentially expressed genes (DEGs) were detected, of which 9605 and 7837 DEGs were identified under cold- and drought- stress, respectively. Functional annotation analysis suggested that stress response mediated by the pathways involving ribosome, phenylpropanoid biosynthesis and biosynthesis of secondary metabolites, among others. Of the obtained DEGs (12,299), 5,143 genes are common to cold- and drought- stress, at least 2248 TFs in 56 TF families were identified that are involved in cold and/or drought treatments during seed germination, including bHLH, NAC, MYB and WRKY families, which suggested that common mechanisms may be originated during maize seed germination in response to different abiotic stresses. This study will provide a better understanding of the molecular mechanism of response to abiotic stress during maize seed germination, and could be useful for cultivar improvement and breeding of high vigour maize cultivars.