A comparative assessment of morphological and molecular characterization among three Ziziphus species

Zarei, Abdolkarim; Rezaei, Asma; Esmailpour, Mohammad; Ebrahimi, Aziz

doi:10.1007/s12298-021-01000-7

Cited by 4 publications

(2 citation statements)

References 60 publications

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“…Molecular studies are essential in detecting genetic variations among genotypes, which helps select diverse parents to successfully carry out a new crossing program. Additionally, the molecular data in conjunction with data generated on morphological variability of current taxa will also be helpful in establishing inter-and intra-population phylogenetic relationships (Zarei et al 2021).…”

Section: Introductionmentioning

confidence: 99%

Assessment of variability among molecular and morphological characters of several wild Rutaceae species

Budiarto,

Martasari,

Mas’udah

et al. 2024

Preprint

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Morphological and molecular assessments were carried out to confirm the genetic variability and relationship among wild Rutaceae species. Thirteen stem and leaves morphological characters and 12 ISSR primers were used to evaluate 17 Rutaceae accessions. The results shows that morphological and molecular assessments confirmed different accessions grouping. Biplot analysis based on morphological characters revealed 3 groups based on the character similarity and 1 distinct group consisting Lunasia sp., M. latifolia, L. amara, E. suaveolens, Clausena sp., and M. minutum with a weak relationship of the observed characters. While the ISSR study divided the accessions into 3 clusters, which include L. acidissima in a distinct cluster. The second cluster composed of Clausena sp, Melicope sp. and Fragara sp. The third cluster was divided into two sub-clusters. The first sub-cluster included M. trichopetala, M. minutum, M. latifolia, L. amara, A. marmelos, Z. ovalifolium, G. pentaphyla, Lunasia sp, Z. rhetsa and M. exotica. While, F. lucida, P. littorale and E. suaveolens are the member of the second sub-cluster. The study has demonstrated the effectiveness of ISSR primers in differentiating species up to the subfamily level and it is crucial to prioritize further research to enhance the specificity of morphological characters.

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Section: Introductionmentioning

confidence: 99%

Assessment of variability among molecular and morphological characters of several wild Rutaceae species

Budiarto,

Martasari,

Mas’udah

et al. 2024

Preprint

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show abstract

“…Over 900 types of jujube cultivars necessitate the vital task of examining and classifying jujube germplasm resources. Traditional study and classification approaches in the study of jujube germplasm are mostly based on morphology [6], but they have shortcomings such as low polymorphism, difficulty in identification, and vulnerability to environmental impacts [7].…”

Section: Introductionmentioning

confidence: 99%

Multiple-Genome-Based Simple Sequence Repeat Is an Efficient and Successful Method in Genotyping and Classifying Different Jujube Germplasm Resources

Li,

Muhammad,

Zhang

et al. 2023

Plants

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Jujube (Ziziphus jujuba Mill.) is a commercially important tree native to China, known for its high nutritional value and widespread distribution, as well as its diverse germplasm resources. Being resilient to harsh climatic conditions, the cultivation of jujube could provide a solution to food insecurity and income for people of arid and semi-arid regions in and outside of China. The evaluation of germplasm resources and genetic diversity in jujube necessitates the use of Simple Sequence Repeat (SSR) markers. SSR markers are highly polymorphic and can be used to evaluate the genetic diversity within and between cultivars of Chinese jujube, and are important for conservation biology, breeding programs, and the discovery of important traits for Chinese jujube improvement in China and abroad. However, traditional methods of SSR development are time-consuming and inadequate to meet the growing research demands. To address this issue, we developed a novel approach called Multiple-Genome-Based SSR identification (MGB-SSR), which utilizes the genomes of three jujube cultivars to rapidly screen for polymorphic SSRs in the jujube genome. Through the screening process, we identified 12 pairs of SSR primers, which were then used to successfully classify 249 jujube genotypes. Based on the genotyping results, a digital ID card was established, enabling the complete identification of all 249 jujube plants. The MGB-SSR approach proved efficient in rapidly detecting polymorphic SSRs within the jujube genome. Notably, this study represents the first successful differentiation of jujube germplasm resources using 12 SSR markers, with 4 markers successfully identifying triploid jujube genotypes. These findings offer valuable information for the classification of Chinese jujube germplasm, thereby providing significant assistance to jujube researchers and breeders in identifying unknown jujube germplasm.

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Cis-element amplified polymorphism (CEAP), a novel promoter- and gene-targeted molecular marker of plants

Chen

Huang

et al. 2022

Physiol Mol Biol Plants

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A comparative assessment of morphological and molecular characterization among three Ziziphus species

Cited by 4 publications

References 60 publications

Assessment of variability among molecular and morphological characters of several wild Rutaceae species

Assessment of variability among molecular and morphological characters of several wild Rutaceae species

Multiple-Genome-Based Simple Sequence Repeat Is an Efficient and Successful Method in Genotyping and Classifying Different Jujube Germplasm Resources

Cis-element amplified polymorphism (CEAP), a novel promoter- and gene-targeted molecular marker of plants

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