A comparative assessment of reference genes in mouse brown adipocyte differentiation and thermogenesis in vitro
Trang Huyen Lai,
Jin Seok Hwang,
Quang Nhat Ngo
et al.
Abstract:Adipogenic differentiation and thermogenesis in brown adipose tissue (BAT) undergo dynamic processes, altering phenotypes and gene expressions. Proper reference genes in gene expression analysis are crucial to mitigate experimental variances and ensure PCR efficacy. Unreliable reference genes can lead to erroneous gene expression quantification, resulting in data misinterpretation. This study focused on identifying suitable reference genes for mouse brown adipocyte research, utilizing brown adipocytes from the… Show more
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