A comparative evaluation of statistical empirical and neural intelligence modeling of Manihot esculenta-derived leaves extract for optimized bio-coagulation-flocculation of turbid water

Kusuma, Heri Septya; Amenaghawon, Andrew N.; Darmokoesoemo, Handoko; Neolaka, Yantus A.B.; Widyaningrum, Bernadeta Ayu; Onowise, Samuel Usiwo; Anyalewechi, Chinedu L.

doi:10.1016/j.indcrop.2022.115194

Cited by 41 publications

(9 citation statements)

References 62 publications

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“…Therefore, employing RSM in the optimization of glycerol carbonate synthesis can not only enhance efficiency but also provide valuable insights into the intricate relationships among reaction parameters, ultimately contributing to improved yields and cost-effectiveness. [33][34][35][36] In the present work, crude glycerol was rst enriched and then the enriched glycerol was used as a starting reactant for GC synthesis. The active species were incorporated onto the MCM-41 framework via impregnation.…”

Section: Introductionmentioning

confidence: 99%

Glycerol carbonate synthesis via transesterification of enriched glycerol and dimethyl carbonate using a Li-incorporated MCM-41 framework

Jitjamnong,

Khongprom,

Ratanawilai

et al. 2024

RSC Adv.

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Section: Introductionmentioning

confidence: 99%

Glycerol carbonate synthesis via transesterification of enriched glycerol and dimethyl carbonate using a Li-incorporated MCM-41 framework

Jitjamnong,

Khongprom,

Ratanawilai

et al. 2024

RSC Adv.

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“…This study introduces a fast and simple method for PET depolymerization. [36][37][38] 2. Experiment and methods…”

Section: Introductionmentioning

confidence: 99%

Optimization of Ti–BA efficiently for the catalytic alcoholysis of waste PET using response surface methodology

Wen

Shen

et al. 2023

RSC Adv.

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“…A common huddle with fermentative production of L-ASNases and other microbially-derived value-added products is low yield because microorganisms maintain tight control over biosynthesis of metabolites, especially extracellularly-secreted ones (Sadh et al 2018 ; Pinu et al 2018 ). A combined use of hyper-producing strains obtained naturally or through mutagenesis, protoplast fusion and genetic engineering, especially with CRISPR/Cas9 system (Zou et al 2019 ; Zhang et al 2021 ; Hu et al 2021 ), and design of experiments by response surface methodology and artificial neural network, has significantly improved yield (Farjaminezhad and Garoosi 2021 ; Asitok et al 2022a ; Kusuma et al 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Production, characterization and techno-economic evaluation of Aspergillus fusant l-asparaginase

et al. 2023

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Protoplast fusion is one of the most reliable methods of introducing desirable traits into industrially-promising fungal strains. It harnesses the entire genomic repertoire of fusing microorganisms by routing the natural barrier and genetic incompatibility between them. In the present study, the axenic culture of a thermo-halotolerant strain of Aspergillus candidus (Asp-C) produced an anti-leukemic l-asparaginase (l-ASNase) while a xylan-degrading strain of Aspergillus sydowii (Asp-S) produced the acrylamide-reduction type. Protoplast fusion of the wild strains generated Fusant-06 with improved anti-leukemic and acrylamide reduction potentials. Submerged fed-batch fermentation was preferred to batch and continuous modes on the basis of impressive techno-economics. Fusant-06 l-ASNase was purified by PEG/Na+ citrate aqueous two-phase system (ATPS) to 146.21-fold and global sensitivity analysis report revealed polymer molecular weight and citrate concentration as major determinants of yield and purification factor, respectively. The enzyme was characterized by molecular weight, amino acid profile, activity and stability to chemical agents. Michaelis–Menten kinetics, evaluated under optimum conditions gave Km, Vmax, Kcat, and Kcat/Km as 6.67 × 10–5 M, 1666.67 µmolmin−1 mg−1 protein, 3.88 × 104 min−1 and 5.81 × 108 M−1.min−1 respectively. In-vitro cytotoxicity of HL-60 cell lines by Fusant-06 l-ASNase improved significantly from their respective wild strains. Stability of Fusant-06 l-ASNase over a wide range of pH, temperature and NaCl concentration, coupled with its micromolar Km value, confers commercial and therapeutic value on the product. Free-radical scavenging and acrylamide reduction activities were intermediate and the conferred thermo-halo-stability could be exploited for sustainable clinical and food industry applications.

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A comparative evaluation of statistical empirical and neural intelligence modeling of Manihot esculenta-derived leaves extract for optimized bio-coagulation-flocculation of turbid water

Cited by 41 publications

References 62 publications

Glycerol carbonate synthesis via transesterification of enriched glycerol and dimethyl carbonate using a Li-incorporated MCM-41 framework

Glycerol carbonate synthesis via transesterification of enriched glycerol and dimethyl carbonate using a Li-incorporated MCM-41 framework

Optimization of Ti–BA efficiently for the catalytic alcoholysis of waste PET using response surface methodology

Production, characterization and techno-economic evaluation of Aspergillus fusant l-asparaginase

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