A comparative histological study of the effect of TheraCal LC and biodentine on direct pulp capping in rabbits: an experimental study

Abstract

Objectives This study histologically compared the effect of TheraCal LC and biodentine on direct pulp capping using a rabbit model. Materials and methods A direct pulp capping procedure was performed on 40 mandibular central incisors of 20 healthy, male New Zealand white rabbits. TheraCal LC and biodentine were applied to exposed pulp and 10 randomly selected rabbits were euthanized after the first and second week. Incisors were extracted and prepared for histological processing and examination to check the dentine bridge thickness, continuity, and extent of pulp inflammation. A blinded data analysis was performed, and groups were compared using a Wilcoxon signed-rank test while changes across time within each group were assessed using the Mann–Whitney U test. Results When comparing the dentine bridge thickness, biodentine showed a significantly thicker dentine bridge in the first and second week (mean 28.16 µm, 33.66 µm), while TheraCal LC showed a dentine bridge in the second week only (mean 15.93 µm). Regarding dentine bridge continuity, biodentine showed a significantly better dentine bridge in the first week. Additionally, there was no difference in the second week. Furthermore, there was no statistically significant difference between each of the materials regarding the extent of inflammation. Conclusions Biodentine in vivo showed better results concerning thickness and continuity of the dentine bridge after direct pulp capping in rabbit incisors. Both biodentine and TheraCal LC had a similar inflammatory effect on the pulp. Clinical relevance Biodentine is more successful as a direct pulp capping material compared to TheraCal LC.

Examination of surface porosity of current pulp capping materials by micro-computed tomography (micro-CT) method

“…This greater porosity of Bioaggregate may be a contributing factor in the dense mineralized dentine formation with Bioaggregate reported in the study by Kim et al [38]. Kayad et al [39] in the second week, while Biodentine created a significantly thicker dentine bridge in the first and second weeks. The results of a randomized clinical trial showed that TheraCal was less successful than Biodentine but better than Dycal when evaluated clinically and radiographically [40].…”

“…Since the DPSCs, which play a role in forming reparative dentine, can be positioned in the porous structure of Bioaggragate, its porous structure may also play a role in the success of pulp capping treatment. However, in in vivo studies [12,33,39,40,48,49] performed with other, less porous pulp capping materials, successful results were obtained. Thus, further studies should be conducted to determine which properties of the materials used in the pulp capping treatment primarily affect the success.…”

Sol–Gel Technologies to Obtain Advanced Bioceramics for Dental Therapeutics

“…Further products marketed with shorter setting times are Biodentine [122][123][124][125][126][127][128][129], Endocem MTA [130][131][132][133], MTA Bio [134,135], EndoSeal MTA [136,137] and MTA Fillapex [138][139][140][141][142][143]. A setting time of as little as 0.3 min has been reported for TheraCal [144][145][146][147][148] because of the use of resin and light cure technology. The radiopacifying agent used is another important element that has been studied for the improvement of these products.…”

Cytotoxic Effect of Tideglusib on Human Fibroblasts: An In Vitro Study

“…6,7 This restoration is required to seal the exposed area and induce differentiation of dental pulp stem cells into odontoblast-like cells for reparative dentine production. 8 The capping material employed in direct capping processes has been identified as a key element in treatment effectiveness.…”

“…9,11,14 MTA disadvantages, such as lengthy setting time and probable discoloration, prompted the development of additional calcium silicatebased polymers, such as biodentine, to address these shortcomings and increase physiochemical qualities. 7,8,14 Tideglusib is a glycogen synthase kinase-3 (GSK-3) inhibitor that belongs to the thiadiazolidine family. It prevents inflammation and neurodegeneration.…”