A comparative study impact of real estate Tenordin and Captosam on the activity of alkaline phosphatase enzyme in patients with chronic high blood pressure

Abstract

A study was conducted on 64 serum samples of blood from people [36 male and 28 female] aged between 48-65 years old, 47 of whom suffer from chronic high blood pressure, and divided the patient samples to the first group using the drug Tenordin, and a second set using drug Captosam, and 17 healthy sample 9 male and 8 Female control group for comparison.
 The results showed a significant increase in the activity of the alkaline phosphatase ALP for the first group, 19.44±2.94 Kind and King/100ml compared with control group 14.24±2.14 Kind and King/100ml. The second group showed a significant decrease enzyme activity, amounting to 7.16 ± 1.90 Kind and King/100ml compared with the control group.
 Three different concentrations of drugs Captosam and Tenordin were prepared and studied their impact on the effectiveness of the enzyme ALP in vitro, and results showed that the drug Tenordin shows a strong activating effect, but the drug Captosam showed remarkable inhibition effect. Also studied the kinetics of the effect of a drug Captosam inhibitor on the activity of the enzyme and the results showed that the value of Km is 6.8965 mmol /L, while the value of Vmax are 12.5 mmol /L min., as results showed when studying the inhibition of the low values ​​of Km and Vmax, as the value of Km is 3.077 mmol /L, while the Vmax value was 5.555 mmol /L min., and shows that the inhibition type is Uncompetitive.