A Comparative Study of a 3D Bioprinted Gelatin-Based Lattice and Rectangular-Sheet Structures

Kumar, Shweta; Tasnim, Nishat; Dominguez, Erick; Allen, Shane C.; Suggs, Laura J.; Ito, Yoshihiro; Joddar, Binata

doi:10.3390/gels4030073

Cited by 15 publications

(21 citation statements)

References 22 publications

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“…This can be attributed to ZnO NPs' consistently smaller average size when compared to TiO 2 NPs, with size possibly affecting the ability to invade bacterial cells. 30 In general, the U.V. irradiation demonstrated an effect on the optical density (p<0.05), signaling the decreased presence of bacteria.…”

Section: Bacterial Growth Inhibitionmentioning

confidence: 96%

“…3D constructs were printed onto petri dishes at 138-207 kPa. 30 Once printed, the gels were each crosslinked again with 100 µL of 0.25 M CaCl 2 for 20 minutes and washed with 1X-PBS to remove residual CaCl 2 .…”

Section: D Printed Gelsmentioning

confidence: 99%

“…To assess and compare the dimensions of the pores within the 3D printed and manually casted gels, crosssectional images were obtained via SEM, as described in previously published studies. [30][31][32][33] Samples were crosslinked, placed in 193.15°K overnight, lyophilized, and sliced to reveal a cross-sectional area. These sections were sputter-coated (Gatan Model 682 Precision etching coating system, Pleasanton, CA, USA) and viewed with SEM (S-4800, Hitachi, Japan).…”

Section: Scanning Electron Microscopy (Sem) Of the Composite Gelsmentioning

confidence: 99%

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<p>Alginate Hydrogels with Embedded ZnO Nanoparticles for Wound Healing Therapy</p>

Cleetus¹,

Alvarez-Primo²,

Fregoso³

et al. 2020

IJN

Self Cite

120

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Introduction: In this in-vitro study, we designed a 3D printed composite of zinc oxide (ZnO) nanoparticles (NPs) with photocatalytic activities encapsulated within hydrogel (alginate) constructs, for antibacterial purposes applicable towards wound healing. We primarily sought to confirm the mechanical properties and cell compatibility of these ZnO NP infused scaffolds. Methods: The antibacterial property of the ZnO NPs was confirmed by hydroxyl radical generation using ultraviolet (U.V.) photocatalysis. Titanium dioxide (TiO 2), a well-known antibacterial compound, was used as a positive control (1% w/v) for the ZnO NP-based alginate constructs and their antibacterial efficacies compared. Among the ZnO group, 3D printed gels containing 0.5% and 1% w/v of ZnO were analyzed and compared with manually casted samples via SEM, swelling evaluation, and rheological analysis. Envisioning an in-vivo application for the 3D printed ZnO NP-based alginates, we studied their antibacterial properties by bacterial broth testing, cytocompatibility via live/dead assay, and moisture retention capabilities utilizing a humidity sensor. Results: 3D printed constructs revealed significantly greater pore sizes and enhanced structural stability compared to manually casted samples. For all samples, the addition of ZnO or TiO 2 resulted in significantly stiffer gels in comparison with the alginate control. Bacterial resistance testing on Staphylococcus epidermidis indicated the addition of ZnO NPs to the gels decreased bacterial growth when compared to the alginate only gels. Cell viability of STO-fibroblasts was not adversely affected by the addition of ZnO NPs to the alginate gels. Furthermore, the addition of increasing doses of ZnO NPs to the alginate demonstrated increased humidity retention in gels. Discussion: The customization of 3D printed alginates containing antibacterial ZnO NPs leads to an alternative that allows accessible mobility of molecular exchange required for improving chronic wound healing. This scaffold can provide a cost-effective and durable antibacterial treatment option.

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Section: Bacterial Growth Inhibitionmentioning

confidence: 96%

Section: D Printed Gelsmentioning

confidence: 99%

Section: Scanning Electron Microscopy (Sem) Of the Composite Gelsmentioning

confidence: 99%

See 1 more Smart Citation

<p>Alginate Hydrogels with Embedded ZnO Nanoparticles for Wound Healing Therapy</p>

Cleetus¹,

Alvarez-Primo²,

Fregoso³

et al. 2020

IJN

Self Cite

120

View full text Add to dashboard Cite

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“…Derivatives for enzymatic crosslinking (white): gelatin-tyramine (U) [4,11], gelatin/tyramine/heparin (V) [4]; Derivatives suitable for photo-oxidation (green): gelatin-FA (W) [54], gelatin-FI (X) [55] gel-FGE (Y) [56,57]; π-π cycloaddition (yellow): gel-MFVF (Z) [58], gel-AC (α) [59], gel-NC (β) [59];…”

Section: Introductionmentioning

confidence: 99%

(Photo-)crosslinkable gelatin derivatives for biofabrication applications

et al. 2019

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Over the recent decades gelatin has proven to be very suitable as an extracellular matrix mimic for biofabrication and tissue engineering applications. However, gelatin is prone to dissolution at typical cell culture conditions and is therefore often chemically modified to introduce (photo-)crosslinkable functionalities. These modifications allow to tune the material properties of gelatin, making it suitable for a wide range of biofabrication techniques both as a bioink and as a biomaterial ink (component).The present review provides a non-exhaustive overview of the different reported gelatin modification strategies to yield crosslinkable materials that can be used to form hydrogels suitable for 2 biofabrication applications. The different crosslinking chemistries are discussed and classified according to their crosslinking mechanism including chain-growth and step-growth polymerization.The step-growth polymerization mechanisms are further classified based on the specific chemistry including different (photo-)click chemistries and reversible systems. The benefits and drawbacks of each chemistry are also briefly discussed. Furthermore, focus is placed on different biofabrication strategies applying inkjet, deposition and light-based additive manufacturing techniques, and the applications of the obtained 3D constructs.

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“…The attention of scientists was also drawn to the influence of pressure on other types of eukaryotic cells such as HeLa [ 32 ], C57BL/6 (mouse mesenchymal stem cells) [ 33 ], L929 (mouse fibroblasts), and isolated hMSCs (human mesenchymal stem cells) [ 7 ]. It is commonly stated, with reference to different eukaryotic cell types, that pressures of less than 100 MPa can lead to reversible stress and intracellular changes.…”

Section: Discussionmentioning

confidence: 99%

Bionic Organs: Shear Forces Reduce Pancreatic Islet and Mammalian Cell Viability during the Process of 3D Bioprinting

Klak¹,

Kowalska²,

Dobrzański³

et al. 2021

Micromachines

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Background: 3D bioprinting is the future of constructing functional organs. Creating a bioactive scaffold with pancreatic islets presents many challenges. The aim of this paper is to assess how the 3D bioprinting process affects islet viability. Methods: The BioX 3D printer (Cellink), 600 μm inner diameter nozzles, and 3% (w/v) alginate cell carrier solution were used with rat, porcine, and human pancreatic islets. Islets were divided into a control group (culture medium) and 6 experimental groups (each subjected to specific pressure between 15 and 100 kPa). FDA/PI staining was performed to assess the viability of islets. Analogous studies were carried out on α-cells, β-cells, fibroblasts, and endothelial cells. Results: Viability of human pancreatic islets was as follows: 92% for alginate-based control and 94%, 90%, 74%, 48%, 61%, and 59% for 15, 25, 30, 50, 75, and 100 kPa, respectively. Statistically significant differences were observed between control and 50, 75, and 100 kPa, respectively. Similar observations were made for porcine and rat islets. Conclusions: Optimal pressure during 3D bioprinting with pancreatic islets by the extrusion method should be lower than 30 kPa while using 3% (w/v) alginate as a carrier.

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A Comparative Study of a 3D Bioprinted Gelatin-Based Lattice and Rectangular-Sheet Structures

Cited by 15 publications

References 22 publications

<p>Alginate Hydrogels with Embedded ZnO Nanoparticles for Wound Healing Therapy</p>

<p>Alginate Hydrogels with Embedded ZnO Nanoparticles for Wound Healing Therapy</p>

(Photo-)crosslinkable gelatin derivatives for biofabrication applications

Bionic Organs: Shear Forces Reduce Pancreatic Islet and Mammalian Cell Viability during the Process of 3D Bioprinting

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