A Comparative Study of Cardiac Preservation with Celsior or University of Wisconsin Solution with or without Prior Administration of Cardioplegia

Ten-hour preservation of guinea pig isolated hearts perfused at low flow with air-saturated Lifor solution at 26°C: comparison to ViaSpan solution

“…Although there are suitable preservation techniques and solutions for the liver and kidney, there remains a need for better methods to protect the heart for periods longer than 4 -5 h (9,10,32,39,50). More popular clinically used solutions include ViaSpan (22, 28, 51) (also called UW solution), HTK (histidine-tryptophan-ketoglutarate based, or Bretschneider solution) (16,41), Celsior (antioxidant-based solution with mannitol, reduced glutathione, plus high Mg 2ϩ , lactobionate, and glutamate) (5,17,20,33,34), and St. Thomas Hospital solution (STH; a high-K ϩ , high-Mg ϩ , low-Ca 2ϩ , lidocainecontaining solution) (4,8,21,25); others are Euro-Collins and Stanford solutions.…”

“…All have limitations on the adequacy and length of protection (43), and their protective effects are dependent on the conditions of the study (19,21,31). Many of the heart studies compare one preservation solution to another (5,8,16,20,21,24,25,30,33,35,(52)(53)(54) and with or without additives, such as channel activators or blockers (11,12,18,26,40,44,46), ion exchange inhibitors (6,42,48), anesthetics (2,40,47), or BDM (20,46,49). For example, Celsior preserved function better than ViaSpan in several studies (29,52), whereas ViaSpan was better than Celsior in others (5,20).…”

“…Our use of ViaSpan as a low-flow perfusate clearly decreased coronary vascular compliance over time. Another problem with these viscous, high-K ϩ solutions as a storage rather than a perfusion medium is that a more typical extracellular-type cardioplegic solution should be given first to flush the vasculature and arrest the heart before the storage solution is perfused; after storage, the solution needs to be again flushed out (5).…”

Low-flow Perfusion of Guinea Pig Isolated Hearts With 26°C Air-saturated Lifor Solution for 20 Hours Preserves Function and Metabolism

“…Many of the heart studies have compared one preservation solution with another,26,29–33,35,39–44 and with or without additives, such as channel activators or blockers,1,11,45–49 exchange inhibitors,50–52 anesthetics,2,9,49 nitric oxide (NO) donors,53 perfluorocarbons54 or BDM 1,30,55. For example, Celsior solution preserved function better than ViaSpan in several studies,44,56 whereas others found that ViaSpan was better than Celsior 29,30. HTK was found to be more protective than ViaSpan in one study,43 whereas another found the reverse to be true 26.…”

Cold Ischaemic Injury in Kidney Transplantation

“…It contains a high sodium concentration with histidine as a buffer, lactobionate and mannitol to prevent oedema and glutathione as an antioxidant. The solution has proved beneficial in heart, liver, pancreas and in kidney transplantation [75][76][77][78].…”