A comparative study of chiral separation of proton pump inhibitors by supercritical fluid chromatography and high‐performance liquid chromatography

Abstract

A comparative study of chiral separation of pantoprazole and rabeprazole is carried out using supercritical fluid chromatography and high‐performance liquid chromatography. The columns used were Chiralpak IA and Chiralpak IE. The best mobile phase in supercritical fluid chromatography was carbon dioxide‐0.2% triethylamine in methanol (60:40) and 0.1% triethylamine in n‐hexane‐ethanol (50:50) in high‐performance liquid chromatography. For supercritical fluid chromatography, values of the retention factor of pantoprazole enantiomers were 3.97 and 4.88. These values for rabeprazole enantiomers were 6.10 and 7.52. The values of separation and resolution factor for pantoprazole and rabeprazole were 1.23 and 1.23 and 2.20 and 3.36, respectively. Similarly, for high‐performance liquid chromatography, the values of retention factor for enantiomers of pantoprazole were 4.02 and 7.32. These values for rabeprazole enantiomers were 5.32 and 7.88, respectively. The values of separation and resolution factor for pantoprazole and rabeprazole were 1.82 and 1.48 and 9.22 and 6.58, respectively. A comparison was carried out, which confirmed supercritical fluid chromatography as the best method due to its fastness, eco‐friendly, and inexpensiveness. The reported methods are effective, efficient, and reproducible and may be used to separate and identify pantoprazole and rabeprazole in any unknown samples.

Chiral separation of terbutaline by supercritical fluid chromatography with peak purity determination by UPLC–MS and modeling for chiral recognition mechanism

“…Among many chiral stationary phases (CSPs), polysaccharide-based CSPs are the best owing to their remarkable recognition powers. [25][26][27][28] During the search for analytical techniques for this purpose, supercritical fluid chromatography (SFC) was considered the best as a consequence of its ease of operation and inexpensive nature. Furthermore, the purity of the peaks was ascertained by Chiral-liquid chromatographymass spectrometry (LC-MS).…”

“…In view of these facts, efforts were made to explore the best method from the economic and environmental point of view. Among many chiral stationary phases (CSPs), polysaccharide‐based CSPs are the best owing to their remarkable recognition powers 25‐28 . During the search for analytical techniques for this purpose, supercritical fluid chromatography (SFC) was considered the best as a consequence of its ease of operation and inexpensive nature.…”

Rapid chiral separation of potential antibiotics using supercritical fluid chromatography

“…These chiral columns such as Chiralpak IA and Chiralpak IG are amylose-based chemistry resulting in efficient enantiomeric separation. 7,8 Therefore, some potential antibiotic drugs such as ofloxacin and cefotaxime were selected for the chiral separation using aforesaid chiral stationary phases (CSPs). Ofloxacin belongs to the fluoroquinolones group and is also known as a synthetic anti-microbial agent (Figure 1).…”

“…Among these, polysaccharide‐based immobilized columns are producing remarkable outputs. These chiral columns such as Chiralpak IA and Chiralpak IG are amylose‐based chemistry resulting in efficient enantiomeric separation 7,8 . Therefore, some potential antibiotic drugs such as ofloxacin and cefotaxime were selected for the chiral separation using aforesaid chiral stationary phases (CSPs).…”