A comparative study of different amniotic membrane orientations during extraocular muscle surgery in rabbits

Kassem, Rehab R.; El-Mofty, Randa Mohamed Abdel-Moneim; Khodeir, Mustafa Mahmoud; Hamza, Wael Mostafa

doi:10.1080/02713683.2017.1401645

Cited by 8 publications

(7 citation statements)

References 27 publications

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“…The authors concluded that any AMT orientation was effective in preventing adhesions, with no advantage seen in either group. 152 Although there is limited evidence within the literature to support a sutured or sutureless technique, one review article recommends the use of a sutureless technique as it induces less of an inflammatory reaction and results in a shorter operating time. 145 Ocular Surface Wound Healing: Patch Chemical/Thermal Injury Amnion has a range of biological properties that make it attractive for use in the treatment of chemical burns, such as its epitheliotrophic; anti-inflammatory and antineovascular effects.…”

Section: Strabismusmentioning

confidence: 99%

Section: Strabismusmentioning

confidence: 99%

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<p>Amniotic Membrane Transplantation in Ophthalmology: An Updated Perspective</p>

Walkden¹

2020

OPTH

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The aim of this paper is to provide a succinct literature review of the different clinical applications for AMT usage in an ophthalmic setting, ranging from commonly used applications to less mainstream approaches. The hope is that this review enables the reader to have a better understanding of the biological properties of amnion as well as the indications and scenarios in which AMT can be used, whilst presenting relevant evidence from within the literature which may be of interest. We also provide an update on the methods of preservation of amniotic membrane and the application methodologies. Methods: Literature search. A PubMed search was performed using the search terms "amniotic membrane transplant", "amnion AND cornea", amnion AND ophthalmology", "amnion AND ocular surface" and "Amnion AND eye". A full review of the literature using the PubMed database was conducted up until 01/05/20. The articles used were written in English, with all articles accessed in full. Both review articles and original articles were used for this review. All full publications related to ophthalmology were considered.

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Section: Strabismusmentioning

confidence: 99%

Section: Strabismusmentioning

confidence: 99%

<p>Amniotic Membrane Transplantation in Ophthalmology: An Updated Perspective</p>

Walkden¹

2020

OPTH

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“…There is a growing body of evidence, evaluating the use of AMs in strabismus surgery. 83 , 84 , 85 , 86 , 87 , 88 , 89 Similar to the present study, a 2019 review article evaluated the use of AMs with different processing methodologies for extraocular muscle surgery. 89 The authors concluded that dried AM does not effectively limit adhesion formation.…”

Section: Discussionmentioning

confidence: 80%

“…78,80,82 There is a growing body of evidence, evaluating the use of AMs in strabismus surgery. [83][84][85][86][87][88][89] Similar to the present study, a 2019 review article evaluated the use of AMs with different processing methodologies for extraocular muscle surgery. 89 The authors concluded that dried AM does not effectively limit adhesion formation.…”

Section: Ddham Supports An Initial Inflammatory Response Followed By ...mentioning

confidence: 93%

An in vitro comparison of human corneal epithelial cell activity and inflammatory response on differently designed ocular amniotic membranes and a clinical case study

Mao

Protzman²,

John

et al. 2022

J Biomed Mater Res

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Amniotic membrane (AM) is a naturally derived biomaterial with biological and mechanical properties important to Ophthalmology. The epithelial side of the AM promotes epithelialization, while the stromal side regulates inflammation. However, not all AMs are equal. AMs undergo different processing with resultant changes in cellular content and structure. This study evaluates the effects of sidedness and processing on human corneal epithelial cell (HCEC) activity, the effect of processing on HCEC inflammatory response, and then a case study is presented. Three differently processed, commercially available ocular AMs were selected: (1) Biovance ® 3L Ocular, a decellularized, dehydrated human AM (DDHAM), (2) AMBIO2 ® , a dehydrated human AM (DHAM), and(3) AmnioGraft ® , a cryopreserved human AM (CHAM). HCECs were seeded onto the AMs and incubated for 1, 4 and 7 days. Cell adhesion and viability were evaluated using alamarBlue assay. HCEC migration was evaluated using a scratch wound assay. An inflammatory response was induced by TNF-α treatment. The effect of AM on the expression of pro-inflammatory genes in HCECs was compared using quantitative polymerase chain reaction (qPCR). Staining confirmed complete decellularization and the absence of nuclei in DDHAM. HCEC activity was best supported on the stromal side of DDHAM. Under inflammatory stimulation, DDHAM promoted a higher initial inflammatory response with a declining trend across time. Clinically, DDHAM was used to successfully treat anterior basement membrane dystrophy. Compared with DHAM and CHAM, DDHAM had significant positive effects on the cellular activities of HCECs in vitro, which may suggest greater ocular cell compatibility in vivo.

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“…To isolate the amnion, several pregnant and parturient New Zealand white rabbits were subjected to cesarean section ( Kassem et al, 2018 ). The amniotic membrane was removed under aseptic conditions.…”

Section: Methodsmentioning

confidence: 99%

Effect of Amniotic Membrane/Collagen-Based Scaffolds on the Chondrogenic Differentiation of Adipose-Derived Stem Cells and Cartilage Repair

Cao

Tong

Wang³

et al. 2021

Front. Cell Dev. Biol.

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Objectives: Repairing articular cartilage damage is challenging. Clinically, tissue engineering technology is used to induce stem cell differentiation and proliferation on biological scaffolds to repair defective joints. However, no ideal biological scaffolds have been identified. This study investigated the effects of amniotic membrane/collagen scaffolds on the differentiation of adipose-derived stem cells (ADSCs) and articular cartilage repair.Methods: Adipose tissue of New Zealand rabbits was excised, and ADSCs were isolated and induced for differentiation. An articular cartilage defect model was constructed to identify the effect of amniotic membrane/collagen scaffolds on cartilage repair. Cartilage formation was analyzed by imaging and toluene blue staining. Knee joint recovery in rabbits was examined using hematoxylin and eosin, toluidine, safranine, and immunohistochemistry at 12 weeks post-operation. Gene expression was examined using ELISA, RT-PCR, Western blotting, and immunofluorescence.Results: The adipose tissue was effectively differentiated into ADSCs, which further differentiated into chondrogenic, osteogenic, and lipogenic lineages after 3 weeks’ culture in vitro. Compared with platelet-rich plasmon (PRP) scaffolds, the amniotic membrane scaffolds better promoted the growth and differentiation of ADSCs. Additionally, scaffolds containing the PRP and amniotic membrane efficiently enhanced the osteogenic differentiation of ADSCs. The levels of COL1A1, COL2A1, COL10A1, SOX9, and ACAN in ADSCs + amniotic membrane + PRP group were significantly higher than the other groups both in vitro and in vivo. The Wakitani scores of the ADSC + amniotic membrane + PRP group were lower than that in ADSC + PRP (4.4 ± 0.44**), ADSC + amniotic membrane (2.63 ± 0.38**), and control groups (6.733 ± 0.21) at week 12 post-operation. Osteogenesis in rabbits of the ADSC + amniotic membrane + PRP group was significantly upregulated when compared with other groups. Amniotic membranes significantly promoted the expression of cartilage regeneration-related factors (SOX6, SOX9, RUNX2, NKX3-2, MEF2C, and GATA4). The ADSC + PRP + amniotic membrane group exhibited the highest levels of TGF-β, PDGF, and FGF while exhibiting the lowest level of IL-1β, IL6, and TNF-α in articular cavity.Conclusion: Amniotic membrane/collagen combination-based scaffolds promoted the proliferation and cartilage differentiation of ADSCs, and may provide a new treatment paradigm for patients with cartilage injury.

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A comparative study of different amniotic membrane orientations during extraocular muscle surgery in rabbits

Abstract: All AM orientations were equally effective in preventing the development of postoperative adhesions between the extraocular muscle and surrounding tissues.

Cited by 8 publications

References 27 publications

<p>Amniotic Membrane Transplantation in Ophthalmology: An Updated Perspective</p>

<p>Amniotic Membrane Transplantation in Ophthalmology: An Updated Perspective</p>

An in vitro comparison of human corneal epithelial cell activity and inflammatory response on differently designed ocular amniotic membranes and a clinical case study

Effect of Amniotic Membrane/Collagen-Based Scaffolds on the Chondrogenic Differentiation of Adipose-Derived Stem Cells and Cartilage Repair

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