A comparative study of E-cadherin and stromelysin-3 expression in de novo and ex adenoma carcinoma of the colorectum

Włodarczyk, Janusz; Bethke, B.; Mueller, Elke; Stolte, Manfred; Mueller, James

doi:10.1007/s004280100468

Cited by 17 publications

(18 citation statements)

References 29 publications

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“…Abnormalities of E-cadherin expression have been described in colorectal carcinoma [28] and have been correlated with increased invasiveness, metastatic potential and poor patient prognosis in this tumor type [29] . Recent research showed that E-cadherin expression was significantly lower in the de novo group tissue than that in the prudent group [30,31] . In the present study, by Western blotting analysis, we also confirmed that LST-R1 cells have a prominent lower expression of E-cadherin than that in several other cell lines.…”

Section: Discussionmentioning

confidence: 95%

Establishment and characterization of a new cell line derived from human colorectal laterally spreading tumor

Wang¹,

Lai²,

Yeung³

et al. 2008

WJG

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CONCLUSION:We established and characterized a colorectal cancer cell line, LST-R1 and LST-R1 has an obvious malignant tendency, which maybe partially attributed to the changes of the expression of some adhesion molecules, such as E-cadherin. It is also a versatile tool for exploring the original and progressive mechanisms of laterally spreading tumor and the early colon cancer genesis. INTRODUCTIONTwo routes have been described that can lead to colon carcinogenesis: one is the adenoma-carcinoma model, which is the main carcinogenetic pathway of prudent colorectal tumor and has been widely accepted in the west. However, an increasing number of superficial or flat colorectal tumors have been reported, particular in Japan [1,2] . The superficial tumors are considered to have a distinct clinicopathologic, genetic feature and behave a different carcinogenetic pathway, called de novo pathway [3][4][5][6] . Laterally spreading tumor (LST) is a unique subtype of superficial colorectal tumor, which was first reported by Kudo S in 1993 [7,8] and many names such as granular cluster type, nodule-aggregating tumor, creeping tumor, superficial spreading (epithelial) tumor, flower-bed-like lesion had been used. In 1998, it was defined as tumors originated from the colorectal mucous membranes and mainly extends laterally rather than vertically with its diameter greater than 1 cm ( Figure 1A) [9][10][11][12] . It has a tendency to affect the rectum, sigmoid colon, and caecum. In some studies, it has been reported that the carcinogenesis rate of LST is 8.64%-52.5% [13][14][15] . Abstract AIM: To study the molecular mechanism of laterally spreading tumor (LST), a cell line [Laterally Spreading Tumor-Rectum 1 (LST-R1)] was derived and the characteristics of this cell line were investigated. BASIC RESEARCH METHODS:A new cell line (LST-R1) originated from laterally spreading tumor was established. Properties of the cell line were characterized using scanning and transmission electron microscopy, immunohistochemistry method, cytogenetic analysis and nude mice xenograft experiments. In vitro invasion assay, cDNA microarray and Western blotting were used to compare the difference between the LST-R1 and other colorectal cancer cell lines derived from prudent colon cancer. RESULTS:Our study demonstrated that both epithelial special antigen (ESA) and cytokeratin-20 (CK20) were expressed in LST-R1. The cells presented microvilli and tight junction with large nuclei. The karyotypic analysis showed hyperdiploid features with structural chromosome aberrations. The in vivo tumorigenicity was also demonstrated in nude mice xenograft experiments. The invasion assay suggested this cell line has a higher invasive ability. cDNA microarray and Western blotting show the loss of the expression of E-cadherin in LST-R1 cells.

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Section: Discussionmentioning

confidence: 95%

Establishment and characterization of a new cell line derived from human colorectal laterally spreading tumor

Wang¹,

Lai²,

Yeung³

et al. 2008

WJG

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“…In a recent publication comparing de novo and ex adenoma carcinomas, we found that E-cadherin was significantly more often less strongly expressed than in a comparable group of ex adenoma carcinomas [50]. Of 42 ex adenoma pT1 carcinomas, 79% retained a normal level of E-cadherin expression compared with just over half of the de novo group (Fig.…”

Section: Cell Adhesion: E-cadherin and Cd44 Expressionmentioning

confidence: 87%

“…We have found that a significantly higher proportion of de novo cases had extensive staining for ST-3 (P=0.014) than in a comparable group of ex adenoma cases (Fig. 13) [50]. ST-3 expression was also significantly associated with more poorly differentiated lesions and showed strong statistical correlation with tumor cell dissociation on the tumor front and, particularly, with the likelihood of vascular invasion.…”

Section: Stromelysin-3 (St-3) Expressionmentioning

confidence: 91%

Colorectal de novo carcinoma: a review of its diagnosis, histopathology, molecular biology, and clinical relevance

2002

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The effort to reduce colorectal adenocarcinoma mortality increasingly depends on detection and removal of precancerous adenomas by colonoscopy. Sporadic reports have described small, aggressive carcinomas that do not appear to develop from adenomas and have been called "de novo" carcinomas. These lesions seem to challenge the basis of colorectal surveillance and are therefore a controversial topic. This review presents the history of the de novo concept, the problems concerned with the histopathologic diagnosis of these lesions and what is presently known about their clinical and molecular biologic features in comparison with the more common ex adenoma type of colorectal carcinomas. This information will show that, despite their rarity, it is important for both pathologists and gastroenterologists to be aware of these lesions.

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“…Generally, carcinoma cells are characterized by poor intercellular adhesion, loss of differentiated epithelial morphology and increased cellular motility [16][17][18]. They also feature downregulation or complete shutdown of Ecadherin expression caused by mutation of the E-cadherin gene (CDH1) or other mechanisms that interfere with the integrity of the adherens junctions [3].…”

Section: Discussionmentioning

confidence: 99%

An Immunohistochemical Study of Colon Adenomas and Carcinomas: E-cadherin, Syndecan-1, Ets-1

Pávai

Dénes

et al. 2009

Pathol. Oncol. Res.

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It is thought that dysregulation of E-cadherin, syndecan-1 (CD138) and Ets-1 is involved in carcinoma development. E-cadherin is an important epithelial cell adhesion molecule; syndecan-1 (CD138) is a regulatory proteoglycan in both cell-cell and cell-matrix adhesion and Ets-1 is a proto-oncogene and transcription factor, which takes part in extracellular matrix remodeling. Our goal was to study the changes in the expression of these molecules during colon carcinoma development and progression. We tested 117 colon adenomas and 149 de novo and ex adenoma carcinomas of the colon, using the Ultravision Polymer system. The positive reaction rate was 100% for E-cadherin, 98.3% for syndecan-1 and 22.4% for Ets-1 in adenomas, while in carcinomas it was 88.5%, 62.4% and 56.3% respectively. We found decreasing expression of E-cadherin and syndecan-1 throughout colon carcinoma progression and an opposite regulation for the Ets-1 protein. Decrease in expression of syndecan-1 is more pronounced in carcinomas compared to E-cadherin. De novo carcinomas have lower E-cadherin and syndecan-1 expression, and higher Ets-1 expression compared to ex adenoma carcinomas. These findings support the hypothesis that there are differences in the carcinogenesis of these tumors.

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A comparative study of E-cadherin and stromelysin-3 expression in de novo and ex adenoma carcinoma of the colorectum

Cited by 17 publications

References 29 publications

Establishment and characterization of a new cell line derived from human colorectal laterally spreading tumor

Establishment and characterization of a new cell line derived from human colorectal laterally spreading tumor

Colorectal de novo carcinoma: a review of its diagnosis, histopathology, molecular biology, and clinical relevance

An Immunohistochemical Study of Colon Adenomas and Carcinomas: E-cadherin, Syndecan-1, Ets-1

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