A Comparative Study of Experimental Rat Models of Renal Calcium Oxalate Stone Formation

Oh, Seung Young; Kwon, Jong Kyou; Lee, Seo Yeon; Ha, Moon Soo; Kwon, Yong Uk; Moon, Young Tae

doi:10.1089/end.2010.0386

Cited by 6 publications

(4 citation statements)

References 24 publications

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“…25,26 In rat models of environmentally induced nephrolithiasis, lithogenic substances must be administered daily via gastric tube after dissolution in salad oil, via intraperitoneal injection, through subcutaneous injection or by a combination of these methods, for periods of up to 28 days. Direct observation of the effects of the various experimental conditions on the formation of crystals in the murine renal tubules requires the use of polarized light microscopy after excision, sectioning, and complex staining of the rat kidneys.…”

Section: Drosophila Models Of Human Nephrolithiaismentioning

confidence: 99%

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Drosophila melanogaster as an Emerging Translational Model of Human Nephrolithiasis

et al. 2013

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Purpose The limitations imposed by human clinical studies and mammalian models of nephrolithiasis have hampered the development of effective medical treatments and preventative measures for decades. The simple but elegant Drosophila melanogaster is emerging as a powerful translational model of human disease, including nephrolithiasis and may provide important information essential to our understanding of stone formation. We present the current state of research using D. melanogaster as a model of human nephrolithiasis. Materials and Methods A comprehensive review of the English language literature was performed using PUBMED. When necessary, authoritative texts on relevant subtopics were consulted. Results The genetic composition, anatomic structure and physiologic function of Drosophila Malpighian tubules are remarkably similar to those of the human nephron. The direct effects of dietary manipulation, environmental alteration, and genetic variation on stone formation can be observed and quantified in a matter of days. Several Drosophila models of human nephrolithiasis, including genetically linked and environmentally induced stones, have been developed. A model of calcium oxalate stone formation is among the most recent fly models of human nephrolithiasis. Conclusions The ability to readily manipulate and quantify stone formation in D. melanogaster models of human nephrolithiasis presents the urologic community with a unique opportunity to increase our understanding of this enigmatic disease.

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Section: Drosophila Models Of Human Nephrolithiaismentioning

confidence: 99%

“…Direct observation of the effects of the various experimental conditions on the formation of crystals in the murine renal tubules requires the use of polarized light microscopy after excision, sectioning, and complex staining of the rat kidneys. 25,26 …”

Section: Drosophila Models Of Human Nephrolithiaismentioning

confidence: 99%

Drosophila melanogaster as an Emerging Translational Model of Human Nephrolithiasis

et al. 2013

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“…Renal calculi formation can be induced experimentally by the administration of various chemicals or by the surgical insertion of a foreign body into the bladder of the experimental animal models (Singh et al, 2010). Calculi are chemically induced by the oral administration of such as ethylene glycol, oxalate, gentamicin sulfate, glycolic acid, ammonium chloride, or L-hydroxyproline (Oh et al, 2011). However, these models are associated with nephrotoxicity and hence, may not represent the actual disease as it appears clinically (Laikangbam and Damayanti, 2012).…”

Section: Discussionmentioning

confidence: 99%

Antiurolithic Activity of Berberis Trifoliata Extract on Induced Urolithiasis in Rats by Zinc Disc Implantation

Pérez-Hernández

Treviño-Moreno

Martínez

et al. 2017

Afr J Tradit Complement Altern Med

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Background:In clinical therapy, there is no satisfactory drug available for treatment of urolithiasis, especially for the prevention of their recurrence. The aim of this work was to evaluate in vivo antiurolithic activity of methanolic extract of Berberis trifoliata leaves. Material and methods: Urolithiasis was induced in Wistar rats by zinc disc implantation in urinary bladder. Upon postsurgical recovery, different doses of the methanolic extract of B. trifoliata leaves (50, 100 and 150 mg/kg body weight) were administered orally to zinc disc implanted rats for a period of 20 days. Antiurolithiatic activity was evaluated by measuring the difference between the weight of the implanted zinc discs at the time of implantation and the final weight of the dried calculi taken out from the bladder at the end of the 20 days period of treatment. Results: Extract of B. trifoliata significantly reduced calculi deposition around the implanted zinc disc at all doses (50, 100, and 150 mg/kg). Conclusion: Treatment with methanolic extract of B. trifoliata is useful agent against the kidney stone formation.

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“…Various species of rats were generally used in urolithiasis experiments [16], and male was more preferable than female animals which was resistant to develop stone in hyperoxaluric condition [17]. In the present study, 8-week-old male Wistar rats were purchased from Nomura International Siam (Thailand), and had been tested for stone inductility using ethylene glycol protocol [18]. All rats were housed under pathogen-free, 12-hour light/dark cycle condition with 21 to 25°C temperature and 40–60% relative humidity and fed by regular diet.…”

Section: Methodsmentioning

confidence: 99%

Urinary sulfated glycosaminoglycan insufficiency and chondroitin sulfate supplement in urolithiasis

et al. 2019

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Familial members of urolithiasis have high risk for stone development. We observed the low sulfated glycosaminoglycan (GAG) excretion in urolithiasis patients and their descendants. In this study, we investigated urinary excretion of sulfated GAG, chondroitin sulfate (CS), heparan sulfate (HS) and hyaluronic acid (HA) in urolithiasis and their children, and explored the effect of CS and HA supplement in urolithic hyperoxaluric rats. The 24-hour urines were collected from urolithiasis patients (28) and their children (40), as well as healthy controls (45) and their children (33) to measure urinary sulfated GAG, CS, HS and HA excretion rate. Our result showed that urinary sulfated GAG and CS were diminished in both urolithiasis patients and their children, while decreased HS and increased HA were observed only in urolithiasis patients. Percentage of HS per sulfated GAG increased in both urolithiasis patients and their children. In hyperoxaluric rats induced by ethylene glycol and vitamin D, we found that CS supplement could prevent stone formation, while HA supplement had no effect on stone formation. Our study revealed that decreased urinary GAG and CS excretion are common in familial members of urolithiasis patients, and CS supplement might be beneficial in calcium oxalate urolithiasis prophylaxis for hyperoxaluric patients.

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A Comparative Study of Experimental Rat Models of Renal Calcium Oxalate Stone Formation

Abstract: Intraperitoneal injection of glyoxylate is a faster, more exact, and more reliable method to produce calcium oxalate crystal deposition in the kidney than previous urolithiasis animal models.

Cited by 6 publications

References 24 publications

Drosophila melanogaster as an Emerging Translational Model of Human Nephrolithiasis

Drosophila melanogaster as an Emerging Translational Model of Human Nephrolithiasis

Antiurolithic Activity of Berberis Trifoliata Extract on Induced Urolithiasis in Rats by Zinc Disc Implantation

Urinary sulfated glycosaminoglycan insufficiency and chondroitin sulfate supplement in urolithiasis

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