A comparative study of in vitro antimicrobial, antioxidant and cytotoxic activity of Albizia lebbeck and Acacia nilotica stem bark

Ali, Mohammad Tuhin; Haque, Sheikh Tanzina; Kabir, Lutful; Rana, Sohel; Haque, Md. Enamul

doi:10.1016/j.bfopcu.2017.10.002

“…(Lawaly et al, 2017). The results of this study is in accordance with previous reports suggesting potent antibacterial activity of A. nilotica (Ali et al, 2017;Singh and Thakur, 2016;Gupta, 2015;Raghavendra et al, 2006), L. inermis Gull et al, 2013;Musa et al, 2011;Akter et al, 2010;Babu and Subhasree, 2009;Al-Rubiay et al, 2008;Nayak et al, 2007) and Z. jujube Linn (Elaloui et al, 2017;Arifa et al, 2016;Naz et al, 2013) against both gram negative and gram positive bacteria.…”

Section: Discussionsupporting

confidence: 93%

Antimicrobial Activities of Acacia nilotica, Ziziphus Jujube Linn and Lawsonia Inermis

Abubakar¹,

Dandare²,

Wasagu³

et al. 2018

Nig. J. Basic Appl. Sci.

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Infectious diseases are important cause of morbidity and mortality due to continuous emergence of microbial resistance to conventional drugs. Acacia nilotica, Ziziphus jujube Linn and Lawsonia inermis are widely used for traditional medicine in Northern Nigeria. However, little is known about the biochemical and microbiological potentials of these indigenous plants. In this study, the plants leaves were screened for phytochemical and in vitro antimicrobial potentials using standard methods. Quantitative phytochemical analysis of crude methanolic leave extracts revealed high content of glycoside, tannins and phenols. High levels of saponins and flavonoids were also detected. The extracts exhibited antibacterial effects on Escherichia coli, Pseudomonas flourecense, Streptococcus and Staphylococcus aureus. At 50 mg/ml extract concentration, the zone of inhibition observed was greater than 6mm. This indicates high inhibitory potency of the plants leaves. In comparison to streptomycin sulphate, A. nilotica and L. inermis had statistically similar (P>0.05) effect on E. coli at 50 mg/ml. In general, the inhibitory effect of A. nilotica and L. inermis were higher than that of Z. jujube Linn in all concentrations, except on E. coli at 150 mg/ml. Both the extracts and control drug had minimum inhibitory concentration of 10 mg/ml for all the microbes tested except Streptococcus (20-25 mg/ml). Furthermore, the average Minimum Bactericidal Concentration was 15 mg/ml except for Streptococcus with 20-25 mg/ml. Methanol extracts of Acacia nilotica, Ziziphus jujube Linn, and Lawsonia inermis exhibit antibacterial effect, hence could be used as sources of potent agents against bacterial infection.

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“…Streptomycin Sulphate The results of this study is in accordance with previous reports suggesting potent antibacterial activity of A. nilotica (Ali et al, 2017;Singh and Thakur, 2016;Gupta, 2015;Raghavendra et al, 2006), L. inermis (Chowdhury et al, 2014;Gull et al, 2013;Musa et al, 2011;Akter et al, 2010;Babu and Subhasree, 2009;Al-Rubiay et al, 2008;Nayak et al, 2007) and Z. jujube Linn (Elaloui et al, 2017;Arifa et al, 2016;Naz et al, 2013) against both gram negative and gram positive bacteria.…”

Section: Discussionsupporting

confidence: 93%

Antimicrobial Activities of Acacia nilotica, Ziziphus Jujube Linn and Lawsonia Inermis

Abubakar¹,

Dandare²,

Wasagu³

et al. 2019

View full text Add to dashboard Cite

Infectious diseases are important cause of morbidity and mortality due to continuous emergence of microbial resistance to conventional drugs. Acacia nilotica, Ziziphus jujube Linn and Lawsonia inermis are widely used for traditional medicine in Northern Nigeria. However, little is known about the biochemical and microbiological potentials of these indigenous plants. In this study, the plants leaves were screened for phytochemical and in vitro antimicrobial potentials using standard methods. Quantitative phytochemical analysis of crude methanolic leave extracts revealed high content of glycoside, tannins and phenols. High levels of saponins and flavonoids were also detected. The extracts exhibited antibacterial effects on Escherichia coli, Pseudomonas flourecense, Streptococcus and Staphylococcus aureus. At 50 mg/ml extract concentration, the zone of inhibition observed was greater than 6mm. This indicates high inhibitory potency of the plants leaves. In comparison to streptomycin sulphate, A. nilotica and L. inermis had statistically similar (P>0.05) effect on E. coli at 50 mg/ml. In general, the inhibitory effect of A. nilotica and L. inermis were higher than that of Z. jujube Linn in all concentrations, except on E. coli at 150 mg/ml. Both the extracts and control drug had minimum inhibitory concentration of 10 mg/ml for all the microbes tested except Streptococcus (20-25 mg/ml). Furthermore, the average Minimum Bactericidal Concentration was 15 mg/ml except for Streptococcus with 20-25 mg/ml. Methanol extracts of Acacia nilotica, Ziziphus jujube Linn, and Lawsonia inermis exhibit antibacterial effect, hence could be used as sources of potent agents against bacterial infection.

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“…Allium ascolinicum sodium phosphate citrate extract showed maximum zone of inhibition 17 mm, 17 mm and 15 mm against Escherichia coli, Proteus vulgaris and Staphylococcus aureus. Ali et al 56 demonstrated the moderate antimicrobial activity Albizia lebbeck extract against the Aspergillus niger , Bacillus subtilis , Vibrio mimicus , Staphylococcus aureus , Salmonella typhi , Shigella dysenteriae and Candida arrizae . Bobby & Wesely 21 also described the antimicrobial activity against Escherichia coli , Bacillus subtilis , Klebsiella pneumonia , Pseudomonas aeruginosa , Proteus vulgaris , S taphylococus aureus and Salmonella typhii.…”

Section: Discussionmentioning

confidence: 99%

Antibacterial Activity of Tris NaCl and PBS Buffer Protein Extract of Cassia fistula, Saccharum officinarum, Albizia lebbeck and Cymbopogon citrates Against Bacterial Strains

Habiba

¹

,

Nisar

²

,

Choohan

³

et al. 2021

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Medicinal plants are gaining popularity over synthetic medicines because antibiotic resistance demands the alternative source of medication. In the present research, the crude protein extraction of 4 medicinal plants Cassia fistula, Saccharum officinarum, Albizia lebbeck and Cymbopogon citrates was carried out. Crude protein extraction was done by 2 different buffers i.e. Tris NaCl buffer and PBS buffer. Protein confirmation was done by Bradford assay in the spectrophotometer. Antibacterial potential was checked and compared against Escherichia coli, Bacillus subtilis, Neisseria gonorrhoea, Bacillus cereus and Proteus mirabilis. Antibacterial assay was performed by disc diffusion method, agar well method and zones of inhibition were calculated. The study results indicated that Tris NaCl extracts’ antimicrobial potential is higher than that of the PBS buffer. On disc diffusion method the Tris NaCl buffer extracts of Cymbopogon citrates showed maximum zone of inhibition 11 mm and 9 mm against Bacillus subtilis and Bacillus cereus respectively and control chloramphenicol showed maximum zone of inhibition 26 mm against Bacillus subtilis. Cassia fistula showed maximum zone of inhibition of 7 mm against Bacillus cereus while Saccharum officinarum and Albizia lebbeck didn’t show the any antibacterial activity. On the other hand, Protein extracts from PBS buffer didn’t show zone of inhibition against any bacteria. Only Albizia lebbeck showed minute zone of inhibition against Neisseria gonorrhea. On well diffusion method, Cassia fistula Tris NaCl protein extract showed the maximum zone of inhibition 20 mm and 18 mm against Proteus mirabilis and Bacillus subtilis respectively. While Albizia lebbeck PBS protein extract showed the maximum zone of inhibition 19 mm and 17 mm against Bacillus subtilis and Bacillus cereus. The results revealed that the protein extract of Albizia lebbeck, Cymbopogon citrates and Cassia fistula can be used tosynthesize antimicrobial drugs to treat the bacterial infections.

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A comparative study of in vitro antimicrobial, antioxidant and cytotoxic activity of Albizia lebbeck and Acacia nilotica stem bark

Cited by 13 publications

References 15 publications

Antimicrobial Activities of Acacia nilotica, Ziziphus Jujube Linn and Lawsonia Inermis

Antimicrobial Activities of Acacia nilotica, Ziziphus Jujube Linn and Lawsonia Inermis

Antimicrobial Activities of <i>Acacia nilotica</i>, <i>Ziziphus Jujube</i> Linn and <i>Lawsonia Inermis</i>

Antibacterial Activity of Tris NaCl and PBS Buffer Protein Extract of Cassia fistula, Saccharum officinarum, Albizia lebbeck and Cymbopogon citrates Against Bacterial Strains

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