A comparative study of Lonicera japonica with related species: Morphological characteristics, ITS sequences and active compounds

Wang, Qingxia; Quan, Qingmei; Zhou, Xueli; Zhu, Yazhi; Lan, Yubin; Li, Shan; Yang, Yu; Zhou, Changyong

doi:10.1016/j.bse.2014.02.002

Cited by 23 publications

(11 citation statements)

References 9 publications

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“…The amounts of luteoloside are remarkably lower in flower buds of L. hypoglauca than inthose of L. japonica , whereas no obvious change is observed between the flower buds of L. japonica and L. macranthoides [ 8 ]. However, according to the description in the Chinese pharmacopoeia 2010 and the view reported by Wu et al [ 7 ], luteolin and luteoloside are in lower abundance in flower buds of L. macranthoides than in those of L. japonica [ 33 ]. In the present study, luteoloside accumulation initially increased from stage 2 to stage 4 and then decreased in stage 5 during flower development in L. macranthoides ( Figure 1 F), which is in accordance with the results of previous reports in L. japonica [ 1 , 7 ].…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis Reveals Molecular Signatures of Luteoloside Accumulation in Senescing Leaves of Lonicera macranthoides

Chen

2018

IJMS

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Lonicera macranthoides is an important medicinal plant widely used in traditional Chinese medicine. Luteoloside is a critical bioactive compound in L. macranthoides. To date, the molecular mechanisms underlying luteoloside biosynthesis are still largely unknown. In this work, high performance liquid chromatography (HPLC) was employed to determine the luteoloside contents in leaves, stems, and flowers at different developmental stages. Results showed that senescing leaves can accumulate large amounts of luteoloside, extremely higher than that in young and semi-lignified leaves and other tissues. RNA-Seq analysis identified that twenty-four differentially expressed unigenes (DEGs) associated with luteoloside biosynthesis were significantly up-regulated in senescing leaves, which are positively correlated with luteoloside accumulation. These DEGs include phenylalanine ammonia lyase 2, cinnamate 4-hydroxylase 2, thirteen 4-coumarate-CoA ligases, chalcone synthase 2, six flavonoid 3′-monooxygenase (F3′H) and two flavone 7-O-β-glucosyltransferase (UFGT) genes. Further analysis demonstrated that two F3′Hs (CL11828.Contig1 and CL11828.Contig2) and two UFGTs (Unigene2918 and Unigene97915) might play vital roles in luteoloside generation. Furthermore, several transcription factors (TFs) related to flavonoid biosynthesis including MYB, bHLH and WD40, were differentially expressed during leaf senescence. Among these TFs, MYB12, MYB75, bHLH113 and TTG1 were considered to be key factors involved in the regulation of luteoloside biosynthesis. These findings provide insights for elucidating the molecular signatures of luteoloside accumulation in L. macranthoides.

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Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis Reveals Molecular Signatures of Luteoloside Accumulation in Senescing Leaves of Lonicera macranthoides

Chen

2018

IJMS

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“…Past research has isolated 140 organic compounds from L. japonica , including essential oils, flavonoids, organic acids, and triterpene glycosides (Kuroda, Shizume, & Mimaki, ; Shang, Pan, Li, Miao, & Ding, ). Recent studies have shown that extracts from L. japonica have a wide range of bioactive properties, such as anti‐inflammatory (Chen, Liou, Tzeng, Lee, & Liu, ), antibacterial (Xiong et al, ), and antioxidant use (Wang et al, ), and are used to treat nephrotic diabetes (Tzeng, Liou, Chang, & Liu, ) and metabolic diseases (Shin et al, ). Tyrosinases (EC1.14.18.1), one of the extracts from L. japonica , are metalloenzymes belonging to the type‐3 (binuclear) copper protein (Rolff, Schottenheim, Decker, & Tuczek, ) that exist widely in microorganisms (Faccio, Kruus, Saloheimo, & Thony‐Meyer, ), animals (Hu, Wang, Deng, & Jiang, ), and plants (Zekiri et al, ).…”

Section: Introductionmentioning

confidence: 99%

Extraction optimization, antioxidant activity, and tyrosinase inhibitory capacity of polyphenols from Lonicera japonica

Fan

Lü

Bai

et al. 2019

Food Science & Nutrition

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The objective of this research was twofold: first, to optimize the extraction process of Lonicera japonica polyphenols using a response surface methodology, and second, to study the antioxidant activity and tyrosinase inhibitory capacity of the polyphenols of different purities. High‐speed shearing homogenization extraction was used to extract the polyphenols from L. japonica . The antioxidant activity and the effect of polyphenols on tyrosinase activity were studied using free radical scavenging assay and the tyrosinase method, respectively. The optimal extraction conditions with an extraction yield of 6.96% for polyphenols were determined as follows: ethanol volume fraction 57%, shearing time 3.30 min, and solid–liquid ratio 1:58. Lonicera japonica polyphenols exhibited potent scavenging activity on 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH) and 2, 2'‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulfonic acid) (ABTS), and inhibitory capacity on tyrosinase. The results suggested that L. japonica polyphenols could be explored as a natural antioxidant and tyrosinase inhibitor.

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“…Alternatively, a gene that is closely related to the variation in the active components can be identified through a study of the functional gene, to provide a basis for further investigation into the genetic mechanisms behind variation in active components (Liu, 2006). Many recent studies on the correlation between gene polymorphisms and active components have suggested that active components are associated with geographical distribution and haplotypes (Li et al, 2011;Wang et al, 2014;Zhang et al, 2015). However, few studies have focused on the correlation between species and active components.…”

Section: Discussionmentioning

confidence: 99%

Analysis of the relationship between ribosomal DNA ITS sequences and active components in Rhodiola plants

Zhang

Yuan

et al. 2016

Genet. Mol. Res.

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Rhodiola plants are a valuable resource in traditional Chinese medicine. The objective of this study was to evaluate the correlation between ribosomal DNA internal transcribed spacer (ITS) sequences and the three active components in Rhodiola plants. For this, we determined ITS sequence polymorphisms and the concentrations of active components salidroside, tyrosol, and gallic acid in different Rhodiola species from the Tibetan Plateau. In a total of 23 Rhodiola samples, 16 different haplotypes were defined based on their ITS sequences. Analysis of the active components in these same samples revealed that salidroside was not detected in species with haplotypes H, H, or H, tyrosol was not detected with haplotypes H, H, H, H, H, or H, and gallic acid was detected in with all haplotypes except H and H. In addition, the concentrations of salidroside, tyrosol and gallic acid varied between samples with different haplotypes as well as those with the same haplotype, implying that no significant correlation exists between haplotype and salidroside, tyrosol or gallic acid concentrations. However, a statistically significant positive correlation was observed for among these three active components.

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A comparative study of Lonicera japonica with related species: Morphological characteristics, ITS sequences and active compounds

Cited by 23 publications

References 9 publications

Transcriptome Analysis Reveals Molecular Signatures of Luteoloside Accumulation in Senescing Leaves of Lonicera macranthoides

Transcriptome Analysis Reveals Molecular Signatures of Luteoloside Accumulation in Senescing Leaves of Lonicera macranthoides

Extraction optimization, antioxidant activity, and tyrosinase inhibitory capacity of polyphenols from Lonicera japonica

Analysis of the relationship between ribosomal DNA ITS sequences and active components in Rhodiola plants

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