A comparative study of microsatellites among crocodiles and development of genomic resources for the critically endangered Indian gharial

Mahfooz, Sahil; Singh, Pallavi; Akhter, Yusuf

doi:10.1007/s10709-021-00148-0

Cited by 4 publications

(1 citation statement)

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“…Using high-throughput sequencing, it was found that the frequency and distribution of microsatellites differ between the Indian gharial ( Gavialis gangeticus ), the American alligator ( Alligator mississippiensis ), and saltwater crocodile ( C. porosus ), and their genomic sequences were dominated by dinucleotide repeats [ 37 ]. Physical mapping on chromosomes was performed for (CGG) 10 microsatellites only, which marked from four to eight chromosomes of Alligatoridae species [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

The Cytogenetic Map of the Nile Crocodile (Crocodylus niloticus, Crocodylidae, Reptilia) with Fluorescence In Situ Localization of Major Repetitive DNAs

Romanenko

Prokopov

Proskuryakova

et al. 2022

IJMS

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Tandemly arranged and dispersed repetitive DNA sequences are important structural and functional elements that make up a significant portion of vertebrate genomes. Using high throughput, low coverage whole genome sequencing followed by bioinformatics analysis, we have identified seven major tandem repetitive DNAs and two fragments of LTR retrotransposons in the genome of the Nile crocodile (Crocodylus niloticus, 2n = 32). The repeats showed great variability in structure, genomic organization, and chromosomal distribution as revealed by fluorescence in situ hybridization (FISH). We found that centromeric and pericentromeric heterochromatin of C. niloticus is composed of previously described in Crocodylus siamensis CSI-HindIII and CSI-DraI repetitive sequence families, a satellite revealed in Crocodylus porosus, and additionally contains at least three previously unannotated tandem repeats. Both LTR sequences identified here belong to the ERV1 family of endogenous retroviruses. Each pericentromeric region was characterized by a diverse set of repeats, with the exception of chromosome pair 4, in which we found only one type of satellite. Only a few repeats showed non-centromeric signals in addition to their centromeric localization. Mapping of 18S–28S ribosomal RNA genes and telomeric sequences (TTAGGG)n did not demonstrate any co-localization of these sequences with revealed centromeric and pericentromeric heterochromatic blocks.

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Section: Introductionmentioning

confidence: 99%