A Comparative Study of Psoriasis and Psoriasiform Dermatoses on Basis of Ki-67 Immunohistochemical Expression

Abdelsalam, Hajir Fathalla Farag; Gobran, Mai Ahmad; Abdelwahab, Mahmoud; Abdelaziz, Hesham Radwan

doi:10.21608/ejhm.2022.218029

Cited by 2 publications

(1 citation statement)

References 10 publications

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“…A Recent study showed that the use of the index of Ki-67 antigen expression with immunohistochemical techniques is one of the most popular indicators of cell proliferation today (24) . In the current study, the level of ki-67 decreased after aescin therapy, suggesting that aescin may at least partially have an antiproliferative impact, these results came in agreement with previous studies (25) . TNF-α is a potent inflammatory cytokine that plays important roles in the initiation and maintenance of inflammation by stimulating the production of other proinflammatory cytokines including IL-6 (26) .…”

Section: Histopathologysupporting

confidence: 93%

Effect of Aescin in Psoriatic-Induced Animal Model: Immunohistochemical and Pathological Study

Wadhah,

Talib,

Ali

et al. 2024

AJPS

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Background: Aescin is a mixture of the triterpene saponins extracted from the seeds of the horse chestnut tree Aesculus hippocastanum. Aescin has a venotonic, anti-inflammatory, antioxidant and anti-edematous characteristics that are mostly connected to the agent molecular mechanism. Objective: The present study aim to investigate the potential effects of Aescin on psoriasis induced by Imiquimod in male rats, ncluding its effect on the level of tumor necrosis factor alpha, Ki-67 and the histopathologic features of the psoriatic skin. Methods: Thirty-six albino male rats were divided into six groups each group containing 6 animals, psoriasis was induced by Imiquimod to five of the groups, while for the last group vasaline was applied and the group served as a control group. The animals were then treated with topical Aescin, topical clobetasol, combination of topical Aescin and clobetasol and oral Aescin, finally all animals were sacrificed and the dorsal back skin was taken to perform histopathological and immunohistochemical analysis. Results: regarding the level of Ki-67, Strong expression of Ki-67 was seen in the group who received Imiquimod only, where the scoring of Ki-67 was notably lowered among the other groups. However, the lowest expression was noticed in the group that were treated with the combination of topical Aescin and clobetasol. While the number of TNF-α positive cells and the intensity of immunostaining were higher in the induction group who received Imiquimod only and the lowest among the group who received the combination of topical Aescin and Clobetasol. Lastly the histopathologic analysis shows that the histopathologic features of psoriasis was markedly affected by the anti-inflammatory effect of Aescin and clobetasol, which was noticed through inhibition of proinflammatory markers, and the decrease in capillary permeability. Conclusion: Topical Aescin alone or in combination with clobetasol reduced Ki-67 expression successfully; furthermore, the combination of topical Aescin and Clobetasol decreased TNF- score and had the strongest anti-inflammatory activity more than the other groups. Lastly Aescin was able to alter the histopathologic features of the psoriatic skin through its anti-inflammatory, venotonic and anti-edematous activity.

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Section: Histopathologysupporting

confidence: 93%

Effect of Aescin in Psoriatic-Induced Animal Model: Immunohistochemical and Pathological Study

Wadhah,

Talib,

Ali

et al. 2024

AJPS

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A Comparative Study of Clinicohistopathological Correlation in Patients of Psoriasis and Psoriasiform Lesions With Ki67 and Cd34 Immunohistochemical Expression

KALYANI VEMAVARAPU,

SAMA SNEHAJA REDDY,

SABBINENI RAMYA

et al. 2024

Asian J Pharm Clin Res

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Objectives: Psoriasis, characterized by chronic inflammation of the skin, stands as a notable example of a psoriasiform tissue pattern. In dermatology, the term “psoriasiform dermatitis” frequently appears across various inflammatory skin conditions, presenting challenges for dermatologists and pathologists alike in accurate differentiation. Relying solely on clinical features may prove insufficient in distinguishing between psoriasis and other psoriasiform dermatoses. Therefore, comprehensive differentiation requires consideration of clinical, histopathological, and immunohistochemical factors. Our study is to compare the clinicohistopathological features of psoriasis and psoriasiform dermatitis and to evaluate the immunohistochemical expression of CD34 and Ki67 in psoriasis and psoriasiform dermatitis. Methods: This retrospective study was conducted at the Department of Pathology, Mediciti Institute of Medical Sciences, Ghanpur, focusing on cases between January 1, 2020, and December 31, 2022. Clinical histories were extracted from requisition forms submitted by the Department of Dermatology. The study analyzed paraffin blocks from 50 patients each diagnosed with psoriasis and psoriasiform dermatitis. Formalin-fixed paraffin-embedded tissue samples were processed into 4-micron sections and initially stained with hematoxylin and eosin. The immunohistochemical analysis included primary antibodies against Ki-67 and CD34, with positive controls sourced from strongly positive samples of squamous cell carcinoma and capillary hemangioma, respectively. Appropriate negative controls were employed to ensure the accuracy of our findings. Results: In our study, we analyzed a total of 100 cases, comprising 50 cases each of psoriasis and psoriasiform dermatitis. Among the psoriasis cases, there was a male predominance with 29 males and 21 females, while psoriasiform dermatitis also showed male predominance with 32 males. The mean age was 33.8 years for psoriasis and 34.8 years for psoriasiform dermatitis. The majority of psoriasis cases fell in the 21–30 years age range, whereas for psoriasiform dermatitis, it was between 31 and 40 years. Significantly, our immunohistochemical analysis revealed that the mean staining intensity of the two markers was notably higher in psoriasis compared to psoriasiform dermatitis. Conclusions: In our study, we observed that the expression levels of Ki-67 and CD34 biomarkers were significantly elevated in psoriasis compared to psoriasiform dermatitis. Notably, four cases of psoriasiform dermatitis demonstrated positivity for CD34 in our analysis. This finding suggests a potential avenue for further research to explore the implications of CD34 positivity in psoriasis.

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A Comparative Study of Psoriasis and Psoriasiform Dermatoses on Basis of Ki-67 Immunohistochemical Expression

Cited by 2 publications

References 10 publications

Effect of Aescin in Psoriatic-Induced Animal Model: Immunohistochemical and Pathological Study

Effect of Aescin in Psoriatic-Induced Animal Model: Immunohistochemical and Pathological Study

A Comparative Study of Clinicohistopathological Correlation in Patients of Psoriasis and Psoriasiform Lesions With Ki67 and Cd34 Immunohistochemical Expression

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