A comparative study of serum myeloperoxidase activity in type 2 diabetes and diabetic nephropathy

Abstract

Inflammatory burden is high in End Stage Renal Disease (ESRD) patients. In Diabetic ESRDpatients, the balance between pro-and anti-oxidant activities is shifted towards an oxidative stress. Myeloperoxidase (MPO), a bactericidal enzyme plays an active role in induction and evolution of the endothelial dysfunction associated with Type 2 Diabetes Mellitus (DM). However, whether MPO can serve as a marker in diabetic ESRD patients is doubtful. Hence, the present study was undertaken. The Fasting Blood Glucose (FBG), Serum Creatinine, Blood Urea Nitrogen (BUN) and Serum Myeloperoxidase levels were estimated in three different groups with 30 subjects each. The Control group includes healthy individuals, group I constitutes Type 2 diabetes with no nephropathy and group II includes Type 2 diabetes with nephropathy patients. Glomerular Filtration Rate (GFR) was calculated using Cockcroft Gault formula corrected for the Body Surface Area. Statistical software, namely, SPSS 15.0, Stata 8.0, MedCalc 9.0.1 and Systat 11.0 were used for the analysis of the data and Microsoft word and Excel have been used to generate graphs, tables etc. Reference Ranges for MPO were established in our study. An increase in MPO levels was noticed in Group I, whereas a differential behaviour of MPO levels was noted in group II. Uremic diabetic nephropathy patients with a low MPO level may be at a lesser risk for any cardiac event compared to those uremic patients with high MPO levels. Hence, MPO may be taken as a biomarker to predict coronary events in diabetic ESRD.

Oxidative Stress and Inflammatory Leukocyte Markers in People with Type 2 Diabetes: A Single Center, Cross-Sectional Study

“…Myeloperoxidase produced by circulating neutrophils plays an important role in both processes of inflammation and oxidative stress [28]. The current study found significantly higher MPO activities in T2D patients, as observed previously [29], suggesting that it might contribute to the enhanced oxidative stress through the generation of ROS.…”

Why there is an Increased Risk of Cardiac Failure, Widening of Pulse Pressure and Hemorrhagic Stroke in Type 2 Diabetics Over Age 60: Roles of Unrecognized Hypomagnesemia and Epigenetics Coupled with Increased Levels of Ceramides, Cytokines, ROS, 4-HNE and Platelet-Activating Factor

“…It has been reported that oxidative stress in T2D patients is also associated with elevated serum and leukocyte levels of myeloperoxidase (MPO) [111][112][113][114]. Using some of our elderly T2D subjects, we have found a strong inverse relationship between RBC Mg 2+ and MPO content (P<0.01) (unpublished findings).…”

“…Using some of our elderly T2D subjects, we have found a strong inverse relationship between RBC Mg 2+ and MPO content (P<0.01) (unpublished findings). MPO is one of the most aggressive oxidants of ROS which usually results in elevated serum levels of hypochlorite (OCl -) which are found in T2D patients [112][113][114][115][116][117][118]. We have reported that OCl -, just like the other ROS, can promote constriction of blood vessels [119][120][121][122][123][124].…”

The Effects of Hydroalcoholic Extract ofArum orientaleon CLP-Induced Sepsis in Rats

“…The activity of MPO, an abundant enzyme of neutrophils, was assessed as previously described 29 with minor modification. Briefly, 1 mL of the serum was mixed with 1 mg of hexadecyltrimethylammonium bromide (HTAB).…”