A comparative study of some more important experimental animal peroxide metabolism enzymes

Matkovics, B.; Novak, Raymond; Hanh, Hoang Duc; Szabó, L.; Varga, Sz.I.; Zalesna, G.

doi:10.1016/0305-0491(77)90218-8

Cited by 21 publications

(6 citation statements)

References 6 publications

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“…CAT activity, found mainly in peroxisomes, is associated with elevated concentrations of H 2 O 2 . These results agree with many other works [58][59][60][61][62] .…”

Section: Discussionsupporting

confidence: 94%

The effects of feeding and starvation on antioxidant defence, fatty acid composition and lipid peroxidation in reared Oncorhynchus mykiss fry

Zengi̇n

2021

Sci Rep

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The effects of feeding and starvation have been studied with respect to oxidative stress and enzymatic antioxidant activities in the whole body of 4 cm rainbow trout fry Oncorhynchus mykiss (Walbaum 1792). The experiment was conducted for 28 days. The selected biomarkers for the study were determined, including non-enzymic scavengers glutathione (GSH), oxidized glutathione (GSSG) and malondialdehyde (MDA) contents and a number of enzymes are known to have major antioxidant activity, such as activities of süperoksit dismutaz (SOD), catalase (CAT), glutatyon peroksidaz (GSHpx), glutatyon Redüktaz (GR) and Glutatyon-S-Transferaz (GST). There is an endogenous cellular glutathione pool which consists of two forms of glutathione, i.e. the GSH and the GSSG. Oxidative damage was measured by the formation of MDA as an indication of lipid peroxidation. The activities of SOD in 14th and 28th day and the activity of CAT in 14th day were increased significantly during the 28 days of starvation. GSHpx and GR activities in starved fry decreased significantly in 28th day. GST activity in all starved fry showed the most significant increases the period of 28 days starving. The highest ΣSFA (Total Saturated Fatty Acid) content was obtained from 28 day starved fry. In starved fry, there was an apparent preference in utilization of C18:1n-9 than in the fed fry. In both starved and fed fry, C16:1n-7 was preferentially kept during the same period. Fry kept 28 days under starvation conditions exhausted C15:0, C17:0, C18:3n-6, C22:0, C24:0. They utilized less C20:5n-3 acid and conserved strongly C22:6n-3 acid. Concentrations of C20:5n-3, C22:5n-3, C22:6n-3 and total n-3 fatty acids significantly increased and C18:3n-3 significantly decreased in the whole body of starved fry during starvation period. A significant increase in the concentrations of C22:5n-3 and C22:6n-3 was determined in the fed fries in the last 2 weeks. Fat-soluble vitamins, cholesterol, stigmasterol and β-sitosterol levels were also determined in the same period of O. mykiss fry.

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“…CAT activity, found mainly in peroxisomes, is associated with elevated concentrations of H 2 O 2 . These results agree with many other works [58][59][60][61][62] .…”

Section: Discussionsupporting

confidence: 94%

The effects of feeding and starvation on antioxidant defence, fatty acid composition and lipid peroxidation in reared Oncorhynchus mykiss fry

Zengi̇n

2021

Sci Rep

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“…To our knowledge the earliest comparison is that of Matkovics et al [13] which determined the activity of antioxidant enzymes. It showed that the activity (per g tissue) of superoxide dismutase and peroxidase was much greater (2-fold on average), while catalase activity was much less in pigeons compared to rats.…”

Section: Introductionmentioning

confidence: 99%

The Long Life of Birds: The Rat-Pigeon Comparison Revisited

2011

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The most studied comparison of aging and maximum lifespan potential (MLSP) among endotherms involves the 7-fold longevity difference between rats (MLSP 5y) and pigeons (MLSP 35y). A widely accepted theory explaining MLSP differences between species is the oxidative stress theory, which purports that reactive oxygen species (ROS) produced during mitochondrial respiration damage bio-molecules and eventually lead to the breakdown of regulatory systems and consequent death. Previous rat-pigeon studies compared only aspects of the oxidative stress theory and most concluded that the lower mitochondrial superoxide production of pigeons compared to rats was responsible for their much greater longevity. This conclusion is based mainly on data from one tissue (the heart) using one mitochondrial substrate (succinate). Studies on heart mitochondria using pyruvate as a mitochondrial substrate gave contradictory results. We believe the conclusion that birds produce less mitochondrial superoxide than mammals is unwarranted.We have revisited the rat-pigeon comparison in the most comprehensive manner to date. We have measured superoxide production (by heart, skeletal muscle and liver mitochondria), five different antioxidants in plasma, three tissues and mitochondria, membrane fatty acid composition (in seven tissues and three mitochondria), and biomarkers of oxidative damage. The only substantial and consistent difference that we have observed between rats and pigeons is their membrane fatty acid composition, with rats having membranes that are more susceptible to damage. This suggests that, although there was no difference in superoxide production, there is likely a much greater production of lipid-based ROS in the rat.We conclude that the differences in superoxide production reported previously were due to the arbitrary selection of heart muscle to source mitochondria and the provision of succinate. Had mitochondria been harvested from other tissues or other relevant mitochondrial metabolic substrates been used, then very different conclusions regarding differences in oxidative stress would have been reached.

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“…Captured spiders were anaesthetised on ice, weighed and homogenised at 0-4°C in 1 : 50 vv, using appropriate buffers: TrisHCl, pH 8 for carboxylesterases (CarE) and glutathione S-transferase (GST) [32], Sørensen buffer, pH 7.85 for catalase (CAT) and superoxide dismutase (SOD) [19,22]. Homogenised samples were centrifuged at 3500 g (Cat and SOD) and 15 000 g for 10 min.…”

Section: Methodsmentioning

confidence: 99%

Metal body burdens and detoxifying enzymes in spiders from industrially polluted areas

Wilczek

Migula

1996

Analytical and Bioanalytical Chemistry

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Detoxifying enzymes have been assayed and metal concentrations have been monitored in four species of spiders from differently contaminated localities in Southern Poland. A behavioural feeding activity and the life style decide on sensitivity and vulnerability of spiders. Wolf-spiders, more active than the web-spinners, cumulate higher amounts of metals, reflecting quantitatively and qualitatively the pollution level in their environment. Detoxifying systems in spiders appeared inducible and efficient to maintain normal physiological responses. Within web-spinning spiders the linyphiids seem to be the most effective regulators of metal burdens. Their detoxifying capabilities correlate well with increased industrial contaminants. The analysis of biochemical biomarkers of exposure confirmed earlier ecological findings that species of the family Linyphiidae are more favoured than the Araneidae. Differences in their feeding activity, behaviour of web-spinning and the size of animals would explain alterations in detoxifying abilities between Meta segmentata and Araneus diadematus.

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A comparative study of some more important experimental animal peroxide metabolism enzymes

Cited by 21 publications

References 6 publications

The effects of feeding and starvation on antioxidant defence, fatty acid composition and lipid peroxidation in reared Oncorhynchus mykiss fry

The effects of feeding and starvation on antioxidant defence, fatty acid composition and lipid peroxidation in reared Oncorhynchus mykiss fry

The Long Life of Birds: The Rat-Pigeon Comparison Revisited

Metal body burdens and detoxifying enzymes in spiders from industrially polluted areas

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