A comparative study of some more important experimental animal peroxide metabolism enzymes

Matkovics, B.; Novak, Raymond; Hanh, Hoang Duc; Szabó, L.; Varga, Sz.I.

doi:10.1016/0305-0491(77)90238-3

Cited by 33 publications

(27 citation statements)

References 4 publications

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“…20 µL of Tsuchiachi solution (chloroform/ethanol 3/5) was added to the supernatant prior to measurement of the enzyme activity. The SOD activity was determined in aliquots by the method of Misra and Fridovics [12] based on the inhibition of transformation of adrenaline to adrenochrome at pH 10.2 [13]. One unit of SOD activity (U) was defined as the amount of enzyme required to cause 50% inhibition of the reaction of adrenaline transformation rate and the results expressed as U mg -1 protein.…”

Section: Methodsmentioning

confidence: 99%

Comparative study of antioxidant capacity in organs of different Allium species

Štajner

Popović

2009

Open Life Sciences

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Cultivated and wild Allium species were investigated in order to compare the antioxidant capacity of their leaves and bulbs. Leaf and bulb anti-oxidative enzymes and scavenger activities, along with quantities of non-enzymic antioxidants, malonyl-dialdehyde and OH radicals were determined. Results obtained suggest that leaves possess higher anti-oxidant and scavenging activities than bulbs in the majority of cultivated and wild Allium species examined. Cultivation of some wild species such as A. flavum L., A. schoenoprasum L. and A. ursinum L. could be conducted in the future in order to produce Allium species with high antioxidant capacity in leaves and bulbs.

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Section: Methodsmentioning

confidence: 99%

Comparative study of antioxidant capacity in organs of different Allium species

Štajner

Popović

2009

Open Life Sciences

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“…Previous studies have shown that the gill is the major site attacked by ROS produced by Chattonella marina (Hishida et al, 1997(Hishida et al, , 1998Nakamura et al, 1998). In contrast, fish erythrocytes are well protected by high enzymatic and non-enzymatic radical scavenging activities (Matkovics et al, 1977;Wdzieczak et al, 1982;Filho et al, 1993). Thus, antioxidant responses in fish gills and erythrocytes were studied in our experiments to detect oxidative stress that might be caused by ROS.…”

Section: Test Speciesmentioning

confidence: 98%

Antioxidant responses and lipid peroxidation in gills and erythrocytes of fish (Rhabdosarga sarba) upon exposure to Chattonella marina and hydrogen peroxide: Implications on the cause of fish kills

Woo

Liu

et al. 2006

Journal of Experimental Marine Biology and Ecology

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Chattonella marina, a red tide or harmful algal bloom species, has caused mass fish kills and serious economic loss worldwide, and yet its toxic actions remain highly controversial. Previous studies have shown that this species is able to produce reactive oxygen species (ROS), and therefore postulated that ROS are the causative agents of fish kills. The present study investigates antioxidant responses and lipid peroxidation in gills and erythrocytes of fish (Rhabdosarga sarba) upon exposure to C. marina, compared with responses exposed to equivalent and higher levels of ROS exposure.Even though C. marina can produce a high level of ROS, gills and erythrocytes of sea bream exposed to C. marina for 1 to 6 h showed neither significant induction of antioxidant enzymes nor lipid peroxidation. Antioxidant responses and oxidative damage did not occur as fish mortality began to occur, yet could be induced upon exposure to artificially supplied ROS levels an order of magnitude higher. The result of this study implies that ROS produced by C. marina is not the principal cause of fish kills.

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“…Guaiacol peroxidase (GPx) activity was determined by the method of Matkovics et al [12], using guaiacol as the substrate at 436 nm. Catalase (CAT) activity was determined by the method of Simon et al [13], at 240 nm.…”

Section: Determination Of Antioxidant Enzyme Activitymentioning

confidence: 99%

Effects of γ-irradiation on antioxidant activity in soybean seeds

2009

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There are some reports that low doses of γ-irradiation could induce antioxidant activities in plant material, including soybean. Irradiation, required for the inactivation of some pathogens and induction of mutations, may have adverse effects on sensorial, nutritional and antioxidant qualities. The effects of different γ-irradiation doses (100-200 Gy) on antioxidant properties of soybean seeds was investigated. In this study, we report the results obtained by analysis of antioxidant enzyme activities, reduced glutathione, malonyldialdehyde (MDA) and hydroxyl (HO˙) radical quantities, soluble protein content, and total antioxidant activity in irradiated soybean seeds. Antioxidant enzyme activities were affected due to high irradiation intensity. Significant changes of total antioxidant activity and MDA and HO˙quantities were observed only under the highest irradiation dose, with a 15.7% reduction in total antioxidant activity, MDA quantity increase of 21.6%, and HO˙ radical quantity increase of 79.3% compared to the non-irradiated control. The total soluble protein content increased slightly.

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A comparative study of some more important experimental animal peroxide metabolism enzymes

Cited by 33 publications

References 4 publications

Comparative study of antioxidant capacity in organs of different Allium species

Comparative study of antioxidant capacity in organs of different Allium species

Antioxidant responses and lipid peroxidation in gills and erythrocytes of fish (Rhabdosarga sarba) upon exposure to Chattonella marina and hydrogen peroxide: Implications on the cause of fish kills

Effects of γ-irradiation on antioxidant activity in soybean seeds

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