A Comparative Study of Some Spectrophotometric and Colorimetric Procedures for the Determination of Serum Glutamic-oxaloacetic and Glutamic-pyruvic Transaminase in Hepatic Diseases

Abstract

Summary. A comparative study has been carried out on the reliability of methods for the determination of both GOT and GPT in serum in the diagnosis of hepatitis. The conventional spectrophotometric methods used by Karmen, Wrobletvski and LaDue and the optimum spectrophotometric methods of Bergmeyer and Bernt have been studied. In addition, the colorimetric methods of Reitman and Frankel, of Reitman modified by King, and of Tonhazy et al., have been examined. The optimum spectrophotometric methods of Bergmeyer and Bernt for GOT and GPT are the only ones that allow the enzymatic reaction to be run at optimum rate. Results obtained by these two methods are most accurate. The conventional spectrophotometric methods of Karmen, Wroblewski and LaDue give lower values than those obtained by the optimum spectrophotometric methods. The Reitman method, carried out with reagents in accordance with information supplied by many manufacturers, does not give reliable results. This is due to the low concentration of substrates in the reaction mixture and to the complex procedure for the preparation of the calibration curve. The method of Tonhazy et al. gives reliable results with a wide range of sensitivity. The advantages of this colorimetric method over Reitman's is due to the high concentration of the substrates, to the decarboxylation of the oxaloacetate to pyruvate in the GOT tests, and to the selective extraction by toluene of the pyruvate-dinitrophenylhydrazone from the reaction mixture.

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