A comparative study of submicron particle sizing platforms: Accuracy, precision and resolution analysis of polydisperse particle size distributions

Anderson, Will; Kozak, Darby; Coleman, Victoria A.; Jämting, Åsa K.; Trau, Matt

doi:10.1016/j.jcis.2013.02.030

Cited by 329 publications

(338 citation statements)

References 25 publications

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“…The mean NPs diameters measured by the Nanoparticle Tracking Analysis System were generally smaller in comparison with those observed by DLS ( Table 2). The comparatively large value reported for NM-300 by Nanoparticle Tracking Analysis System can be related to the limit of detection of the method, which is not reliable for nanoparticles typically below 30-50 nm depending on the NPs optical properties [27][28][29]. Results obtained for NM-300 size distribution by DLS were comparable with those previously observed [30] and indicate a polydispersity of the sample, and therefore an unreliable value of the size measured by DLS [31].…”

Section: Ag Nps Characterizationmentioning

confidence: 57%

Silver nanoparticles induce cytotoxicity, but not cell transformation or genotoxicity on Balb3T3 mouse fibroblasts

Broggi¹,

Ponti²,

Giudetti

et al. 2013

BioNanoMaterials

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Silver nanoparticles (Ag NPs) are one of the most common nanomaterials present in nanotechnologybased products. Here, the physical chemical properties of Ag NPs suspensions of 44 nm, 84 nm and 100 nm sizes synthesized in our laboratory were characterized. The NM-300 material (average size of 17 nm), supplied by the Joint Research Centre Nanomaterials Repository was also included in the present study. The Ag NPs potential cytotoxicity was tested on the Balb3T3 cell line by the Colony Forming Efficiency assay, while their potential morphological neoplastic transformation and genotoxicity were tested by the Cell Transformation Assay and the micronucleus test, respectively. After 24 h of exposure, NM-300 showed cytotoxicity with an IC50 of 8 µM (corresponding to 0.88 µg/mL) while for the other nanomaterials tested, values of IC50 were higher than 10 µM (1.10 µg/mL). After 72 h of exposure, Ag NPs showed size-dependent cytotoxic effect with IC50 values of 1.5 µM (1.16 µg/mL) for NM-300, 1.7 µM (1.19 µg/mL) for Ag 44 nm, 1.9 µM (0.21 µg/mL) for Ag 84 nm and 3.2 µM (0.35 µg/mL) for Ag 100 nm. None of the Ag NPs tested was able to induce either morphological neoplastic transformation or micronuclei formation.

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Section: Ag Nps Characterizationmentioning

confidence: 57%

Silver nanoparticles induce cytotoxicity, but not cell transformation or genotoxicity on Balb3T3 mouse fibroblasts

Broggi¹,

Ponti²,

Giudetti

et al. 2013

BioNanoMaterials

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“…For this reason, care should be taken when choosing a measurement technique [45]. In cases of complex multimodal samples, TRPS can have a greater resolving power than PTA, where it is able to analyse all particles present in the sample [45]. …”

Section: Concentration Measurementsmentioning

confidence: 99%

Characterisation of particles in solution – a perspective on light scattering and comparative technologies

Maguire

Rösslein

Wick

et al. 2018

Science and Technology of Advanced Materials

246

133

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We present here a perspective detailing the current state-of-the-art technologies for the characterisation of nanoparticles (NPs) in liquid suspension. We detail the technologies involved and assess their applications in the determination of NP size and concentration. We also investigate the parameters that can influence the results and put forward a cause and effect analysis of the principle factors influencing the measurement of NP size and concentration by NP tracking analysis and dynamic light scattering, to identify areas where uncertainties in the measurement can arise. Also included are technologies capable of characterising NPs in solution, whose measurements are not based on light scattering. It is hoped that the manuscript, with its detailed description of the methodologies involved, will assist scientists in selecting the appropriate technology for characterising their materials and enabling them to comply with regulatory agencies’ demands for accurate and reliable NP size and concentration data.

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“…Future work could use local ensembles to further extend this work to nonuniform bulk flows [31]. Gains in the accuracy of NTA for dilute, polydisperse suspensions are important because the reliability with which NTA measures polydisperse PSDs remains an open question [41,44]. For example, recent data characterizing patient placental extracellular vesicles using NanoSight R demonstrated that applied flow significantly affected results, but the study did not determine whether the difference was physical (issue with chamber design) or analytical (issue with algorithm) [45].…”

Section: Discussionmentioning

confidence: 99%

Decorrelation correction for nanoparticle tracking analysis of dilute polydisperse suspensions in bulk flow

Hartman

Kirby

2017

Phys. Rev. E

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Nanoparticle tracking analysis, a multi-probe single particle tracking technique, is a widely used method to quickly determine the concentration and size distribution of colloidal particle suspensions. Many popular tools remove non-Brownian components of particle motion by subtracting the ensemble-averaged displacement at each time step, which is termed 'de-drifting'. Though critical for accurate size measurements, de-drifting is shown here to introduce significant biasing error and can fundamentally limit the dynamic range of particle size that can be measured for dilute, heterogeneous suspensions, such as biological extracellular vesicles. We report a more accurate estimate of particle mean-squared displacement, which we call Decorrelation Analysis, that accounts for correlations between individual and ensemble particle motion, which are spuriously introduced by de-drifting. Particle tracking simulation and experimental results show that this approach more accurately determines particle diameters for low concentration, polydisperse suspensions when compared with standard de-drifting techniques.

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A comparative study of submicron particle sizing platforms: Accuracy, precision and resolution analysis of polydisperse particle size distributions

Cited by 329 publications

References 25 publications

Silver nanoparticles induce cytotoxicity, but not cell transformation or genotoxicity on Balb3T3 mouse fibroblasts

Silver nanoparticles induce cytotoxicity, but not cell transformation or genotoxicity on Balb3T3 mouse fibroblasts

Characterisation of particles in solution – a perspective on light scattering and comparative technologies

Decorrelation correction for nanoparticle tracking analysis of dilute polydisperse suspensions in bulk flow

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