A comparative study of Taxol production in liquid and solid-state fermentation with Nigrospora sp. a fungus isolated from Taxus globosa

Abstract

Aims: To determine in liquid (LF) and solid‐state fermentation (SSF) the effect of medium concentration on growth and Taxol produced by Nigrospora sp., a fungus isolated from the Mexican yew. Methods and Results: Nigrospora sp. was grown at different concentrations of the base culture medium M1D, i.e. two (2×), four (4×), six (6×) and eight times (8×) the base concentration. The titres of Taxol determined by competitive inhibition enzyme immunoassay increased with increasing medium concentration in LF and SSF but were higher in SSF in every medium concentration. The Taxol produced in SSF and LF with 8× medium was 221 and 142 ng l−1. The SSF gave also higher biomass, growth and sugar utilization than LF in every medium. The growth and sugar consumption were modelled by the logistic and the Pirt models, respectively. However, the Luedeking–Piret model was unsuitable for Taxol. Conclusions: The SSF surpassed LF in terms of Taxol, growth and sugar utilization; thus, it has significant advantages over LF. Significance and Impact of the Study: This is the first report on Taxol production by SSF and the first contribution to evaluate the influence of the medium on Taxol production in LF and SSF.

Production and characterization of bioactive metabolites from piezotolerant deep sea fungusNigrosporasp. in submerged fermentation

“…), taxol (Ruiz‐Sanchez et al . ), anthraquinone compound ( Chen et al . ), isochromene derivative (Metwaly et al .…”

A fungal endophyte induces transcription of genes encoding a redundant fungicide pathway in its host plant

“…Taxol was verified and quantified either by a competitive immunoassay procedure [42,43], TLC spot densitometry [44,45] or by HPLC-UV. Additional details have been published [16] and are in an Additional file [see Additional file 9].…”

Effects of Cultural Conditions in Enhancing the Production of Anti-MRSA Activity of Lasiodiplodia pseudotheobromae IBRL OS-64, an Endophytic Fungus Isolated from Leaf of Ocimum sanctum L. in Submerged Fermentation System

“…All the needed nutrients such as nitrogen and carbon sources are supplied in to this system to support microbial growth. This fermentation processes normally are done in shake flask or bioreactor whereby the pH, temperature, agitation speed, mixing frequency are controlled (Favella-Torres et al, 1998;Ruiz-Sanchez et al, 2010). According to Vidyalakshmi et al, (2009), submerged culture has their own advantages such as easy to sterilize and controlling process.…”