A comparative study of the drying evolution and dried morphology of two globular proteins in de-ionized water solutions

Pal, Anusuya; Gope, Amalesh; Athair, Ari S.; Iannacchione, Germano S.

doi:10.1039/d0ra01748e

Cited by 22 publications

(46 citation statements)

References 34 publications

(44 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2 (II)a–c). Similar saturation and monotonic behavior are commonly observed in the aqueous protein solutions 38 . The peak is observed from 250 to 400 s at (v/v) (Fig.…”

Section: Resultssupporting

confidence: 79%

“…During this time, the width of the corona region decreases with increasing dilution (decreasing ). The “coffee-ring” behavior 37 (similar to what is typically observed in other protein aqueous solutions 38 ) becomes evident in samples from of 62 to (v/v). V1 - V7 in the Supplementary Information show movies of the drying droplet for samples from 100 to 12.5% (v/v), respectively.…”

Section: Resultssupporting

confidence: 66%

See 1 more Smart Citation

Concentration-driven phase transition and self-assembly in drying droplets of diluting whole blood

Pal

Gope

Obayemi

et al. 2020

Sci Rep

Self Cite

View full text Add to dashboard Cite

Multi-colloidal systems exhibit a variety of structural and functional complexity owing to their ability to interact amongst different components into self-assembled structures. This paper presents experimental confirmations that reveal an interesting sharp phase transition during the drying state and in the dried film as a function of diluting concentrations ranging from 100% (undiluted whole blood) to 12.5% (diluted concentrations). An additional complementary contact angle measurement exhibits a monotonic decrease with a peak as a function of drying. This peak is related to a change in visco-elasticity that decreases with dilution, and disappears at the dilution concentration for the observed phase transition equivalent to 62% (v/v). This unique behavior is clearly commensurate with the optical image statistics and morphological analysis; and it is driven by the decrease in the interactions between various components within this bio-colloid. The implications of these phenomenal systems may address many open-ended questions of complex hierarchical structures.

show abstract

“…2 (II)a–c). Similar saturation and monotonic behavior are commonly observed in the aqueous protein solutions 38 . The peak is observed from 250 to 400 s at (v/v) (Fig.…”

Section: Resultssupporting

confidence: 79%

Section: Resultssupporting

confidence: 66%

Concentration-driven phase transition and self-assembly in drying droplets of diluting whole blood

Pal

Gope

Obayemi

et al. 2020

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…Figure 4 (I,II)a–f illustrates the various stages of the BSA droplets’ drying process. The concentrated (

of 20 wt% in Figure 4 (I)a–f and the most diluted (1 wt% in Figure 4 (II)a–f) samples are selected for studying the drying evolution at T of 55, and

C. After the droplets are pipetted ( Figure 4 (I,II)a), the fluid front recedes from the periphery towards the central region ( Figure 4 (I,II)b–d); similar to what is also observed in the blood droplets ( Figure 2 (I,II)b,c) [ 22 , 25 ]. This indicates that the first stage is a characteristic of a drying bio-colloidal droplet, which does not depend on the constituent particles’ type.…”

Section: Resultsmentioning

confidence: 86%

“…Unlike the blood sample ( Figure 1 ), all BSA’s dried films show a uniform light gray texture. The “coffee-ring” [ 31 ] like behavior is seen at every

[ 25 , 32 ]. These films exhibit a shadowy dark texture around the inner edge of the ring.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Temperature and Concentration Dependence of Human Whole Blood and Protein Drying Droplets

2021

Self Cite

View full text Add to dashboard Cite

The drying of bio-colloidal droplets can be used in many medical and forensic applications. The whole human blood is the most complex bio-colloid system, whereas bovine serum albumin (BSA) is the simplest. This paper focuses on the drying characteristics and the final morphology of these two bio-colloids. The experiments were conducted by varying their initial concentrations, and the solutions were dried under various controlled substrate temperatures using optical and scanning electron microscopy. The droplet parameters (the contact angle, the fluid front, and the first-order image statistics) reveal the drying process’s unique features. Interestingly, both BSA and blood drying droplets’ contact angle measurements show evidence of a concentration-driven transition as the behavior changes from non-monotonic to monotonic decrease. This result indicates that this transition behavior is not limited to multi-component bio-colloid (blood) only, but may be a phenomenon of a bio-colloidal solution containing a large number of interacting components. The high dilution of blood behaves like the BSA solution. The ring-like deposition, the crack morphology, and the microstructures suggest that the components have enough time to segregate and deposit onto the substrate under ambient conditions. However, there is insufficient time for evaporative-driven segregation to occur at elevated temperatures, as expected.

show abstract

Statistical Image Analysis of Drying Bovine Serum Albumin Droplets in Phosphate Buffered Saline

Pal

Gope

Iannacchione

2021

Biomedical Data Mining for Information Retrieval

Self Cite

View full text Add to dashboard Cite

A bio-colloidal drying droplet can be used as a pre-diagnostic technique. However, a successful clinical setting requires a fundamental understanding of the final morphology and the way it is related to the initial state of the constituents present in the droplet. This chapter focuses on the physics associated with different pattern formations in the globular protein, bovine serum albumin (BSA) at different phosphate buffered saline concentrations. The study reports that the first-order statistics (FOS) and the gray level cooccurrence matrix (GLCM) analysis are capable of capturing structural changes of the droplets.While the FOS of the image depend on the individual pixels, the GLCM summarizes both tonal and structural relationships between the neighboring pixels. The horizontal and the vertical orientations of the GLCM parameters show a non-significant effect when the pixel displacement is ≤ 1. Interestingly, two local equilibrium-like regions (the rim and the central regions) appear when these droplets approach the steady state. The bimodal distribution confirms that the BSA-BSA interactions are dominant (recessive) over the BSA-saline interactions in the rim (central) 2 regions that result in the phase separated aggregation of the BSA particles in the presence of the salts.

show abstract

A comparative study of the drying evolution and dried morphology of two globular proteins in de-ionized water solutions

Abstract: Signature pattern formation in drying globular protein solution droplets: understanding self-assembled macroscopic states as indicators of the initial microscopic states.

Cited by 22 publications

References 34 publications

Concentration-driven phase transition and self-assembly in drying droplets of diluting whole blood

Concentration-driven phase transition and self-assembly in drying droplets of diluting whole blood

Temperature and Concentration Dependence of Human Whole Blood and Protein Drying Droplets

Statistical Image Analysis of Drying Bovine Serum Albumin Droplets in Phosphate Buffered Saline

Contact Info

Product

Resources

About