A comparative study of the tryptase release test and the cellular allergen stimulation test (CAST) in mite sensitive patients

Re, Rossi; Monasterolo, Giorgio; Operti, D.

doi:10.1046/j.1365-2222.1998.00310.x

Cited by 11 publications

(12 citation statements)

References 12 publications

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“…produces specific IgE antibodies, while the in vitro reactivity of human basophils may be considered a reflection of clinical status (Lichtenstein and McGlashan 1986). The performance of the CAST in human diagnostic application is comparable to that obtained in the horse; for allergy to mites, Rossi et al (1998) reported a sensitivity and specificity of the CAST with Dermatophagoides farinae of 71% and 87%, respectively.…”

Section: Discussionmentioning

confidence: 96%

Evaluation of an in vitro sulphidoleukotriene release test for diagnosis of insect bite hypersensitivity in horses

Baselgia¹,

Doherr²,

Mellor

et al. 2006

Equine Veterinary Journal

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Summary Reasons for performing study: Insect bite hypersensitivity (IBH) is an IgE‐mediated allergic dermatitis caused by bites of Culicoides and Simulium species, and improved means of diagnosis are required. Objectives: The cellular antigen simulation test (CAST®) with C. nubeculosus and S. vittatum extracts was assessed in a population of IBH‐affected and healthy horses. Variations in test results over a one year period and possible cross‐reactivity between different insect extracts was studied. Methods: A total of 314 mature horses were studied using the CAST. Influence of severity of clinical signs, gender and age were evaluated, and 32 horses were tested repeatedly over one year. The kappa reliability test was used to assess agreement of the test results with different insect extracts. Results: Horses with IBH had significantly higher sLT release than controls with C. nubeculosus and S. vittatum. The highest diagnostic sensitivity and specificity levels were attained when using adult C. nubeculosus extracts with the CAST (78% and 97%, respectively), suggesting that most horses with IBH are sensitised against Culicoides allergens. A proportion of IBH‐affected horses was found to be sensitised to allergens of Simulium spp. in addition to those of C. nubeculosus. The CAST with C. nubeculosus had positive and negative predictive values ≥80% for a true prevalence of IBH of 12–52%. In the follow‐up study, the proportion of IBH‐affected horses with a positive test result ranged from 90% in November to 68% in March. Severity of clinical signs or age did not influence test results significantly. However, IBH‐affected males achieved significantly more positive test results than IBH‐affected females. Conclusions: The CAST with adult C. nubeculosus has high specificity and good sensitivity for diagnosis of IBH. Horses with IBH are mainly sensitised to Culicoides allergens, and some horses are additionally also sensitised to allergens in Simulium spp. Potential relevance: The CAST is likely to be a useful test for diagnosis of IBH, even allowing the identification of IBH‐affected but asymptomatic horses. This test may also help in further characterisation of allergens involved in this condition.

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Section: Discussionmentioning

confidence: 96%

Evaluation of an in vitro sulphidoleukotriene release test for diagnosis of insect bite hypersensitivity in horses

Baselgia¹,

Doherr²,

Mellor

et al. 2006

Equine Veterinary Journal

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“…Using a cut‐off of 5% as positive the overall sensitivity was 51.4%, with 10% as the cut‐off this dropped to 22.8%; the specificities were 63% and 79.5%, respectively. Receiver operating characteristic curves should be employed in order to determine the appropriate cut‐off points [8].…”

Section: Non‐commercial Assays For Determination Of Basophil Activationmentioning

confidence: 99%

“…Similar findings were made by Rossi et al . [8] in a series of 247 mite sensitve patients. The assay appeared to offer good diagnostic performance for D pteronyssinus and D farinae with positive predictive values quoted of 88% and 91%, respectively.…”

Section: Commercial Assays For Determination Of Basophil Activationmentioning

confidence: 99%

Basophil histamine release tests in the diagnosis of allergy and asthma

Crockard

Ennis

2001

Clin Experimental Allergy

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“…Tests based on CysLT release after allergen challenge may be interesting but further studies are required (1291)(1292)(1293). More data are needed to fully appreciate the value of these tests.…”

Section: In Vitro Testsmentioning

confidence: 99%