A comparative study of the TF-Test®, Kato-Katz, Hoffman-Pons-Janer, Willis and Baermann-Moraes coprologic methods for the detection of human parasitosis

Carvalho, Gabriela Lanna Xavier de; Moreira, Luciano Evangelista; Pena, João Luiz; Marinho, Carolina Coimbra; Bahia, Maria Terezinha; Machado-Coelho, George Luiz Lins

doi:10.1590/s0074-02762012000100011

Cited by 69 publications

(75 citation statements)

References 9 publications

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“…Strongyloides stercoralis infestation rates vary widely between studies due to the differences in the source of study participants (Hospital-based, refugee camps and community studies) and sensitivity of the diagnostic tests. (8) (12)(13)(14) Our reports on the prevalence matches with reports from various parts of the world. (5)(6)(7) We also found out that a large number of infested patients were males corroborating a recent report by Azira et al (15) More likelihood of alcohol consumption, eating behaviour and outside activities may be plausible reasons for the noted gender difference in the infestation rate.…”

Section: Discussionsupporting

confidence: 88%

Strongyloides Stercoralis and Its Risk Factors: An Experience at a Tertiary Care Hospital

Kuyare¹,

Koticha²,

Mehta³

2016

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Strongyloides stercoralis is one of the most neglected tropical parasitic infestations. The present study was carried out considering the paucity of data available from Indian context regarding the prevalence and risk factors associated with Strongyloides stercoralis infestation. METHODSThe present study was an observational, retrospective audit/analysis where the hospital records of stool specimens received in Parasitology division for detection of intestinal parasites over a period of three years (January 2011 to December 2013) were analysed. The clinical features and laboratory data of patients infected with larvae of Strongyloides stercoralis were recorded. Descriptive statistics was applied to represent the categories of risk factors in percentages and chi-square test for its association with parasitic infection. RESULTSLarvae of Strongyloides stercoralis were detected in 0.4% (65/15530) stool specimens with a male preponderance. The most common presenting complaints by patients were attributed to diarrhoea 26/65 (40%) and 14/65 (21.5%) patients were asymptomatic. Peripheral eosinophilia was seen in 10.8% (7/65) patients; 52/65 (80%) of patients were identified to have one or more of the known risk factors for Strongyloidiasis of which nearly half gave history of alcoholism (29/52,55.8%); 3/65 (4.6%) patients had hyperinfection of which one died. All three patients had two risk factors (alcoholism and steroids). Oral albendazole was used to treat the parasitic infestation in 31 (48.4%), whereas 34 (51.6%) received oral ivermectin. CONCLUSIONThe study findings of prevalence, clinical features and associated risk factors for Strongyloidiasis can be considered as preliminary report from Western Indian population. The presence of two risk factors in all cases of hyperinfection calls for further studies.

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Section: Discussionsupporting

confidence: 88%

Strongyloides Stercoralis and Its Risk Factors: An Experience at a Tertiary Care Hospital

Kuyare¹,

Koticha²,

Mehta³

2016

jemds

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“…The TF-Test (Three Fecal Test) method was used. It utilizes centrifugation, filtration and sedimentation and has a high sensitivity in detecting protozoa cysts [37].This method was also selected because it allows a prolonged sample preservation time (30 days) without the need for refrigeration. In each stage, three fecal samples were collected from the same subject on alternate days in a 5 day period and placed in separate vials.…”

Section: Methodsmentioning

confidence: 99%

Reducing Occurrence of Giardia duodenalis in Children Living in Semiarid Regions: Impact of a Large Scale Rainwater Harvesting Initiative

et al. 2014

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BackgroundIn Brazil, about two million people living in rural semiarid regions were benefited with the construction of rainwater cement cisterns, as an initiative from the program “One Million Cisterns” (P1MC). Nevertheless, few epidemiological studies have been conducted to assess health risks or protection effects associated with consumption of this water source. The aim of this study was to evaluate whether access to rainwater harvesting cisterns is associated with the decrease in the occurrence of Giardia duodenalis infections in children, compared to other children living in households supplied by other water sources.Methodology/Principal FindingsA quasi-experimental study with two concurrent cohorts was developed in two rural municipalities of the semiarid region of Brazil. A sample of 664 children, aged between 4 months and 5 years old, was followed up, of which 332 had access to rainwater cisterns (cistern group) and 332 did not, having water supplied from alternative sources (comparison group). In a period of approximately one year (2010) intestinal parasites were investigated in feces three times. The prevalence of G. duodenalis in children from the cistern group ranged from 4.8 to 10.5%, while the prevalence in the comparison group ranged from 7.6 to 16.7%. Multivariate analysis (GEE) showed a higher risk of G. duodenalis infection in children who did not have access to rainwater cisterns, when compared to children who did (OR 1.72; 95% CI 1.14–2.59). The other variables associated with G. duodenalis infection were: number of rooms per house (OR 0.89; 95% CI 0.80–0.99); family income (OR0.48; 95% CI 0.26–0.88); birth order (OR 1.72; 95% CI 1.17–2.51); preterm children (OR 1.70; 95% CI 1.19–2.43); and improper hand hygiene prior to food preparation (OR 4.78; 95% CI 1.95–11.76).Conclusions/SignificanceOwnership of a rainwater cistern is associated with a lower prevalence of G. duodenalis infection in children after adjustment for environmental and family-related factors. Nevertheless, the study suggests the necessity to complement physical interventions with actions related to personal and domestic hygiene to enable further reductions in parasite infections affecting mainly the underprivileged populations.

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“…As variáveis observadas foram tipo de parede, revestimento, cobertura, piso, eletricidade; origem e conservação 16 , sendo as amostras fecais coletadas em três dias alternados, em tubos independentes, contendo formol 10%, unificados para dupla filtragem por centrifugação. Esse método foi utilizado por apresentar sensibilidade semelhante ao Kato-Katz para a identificação de ovos de helmintos e maior sensibilidade que os métodos de sedimentação para a identificação de cistos de protozoários 16 . No laboratório de parasitologia da Universidade Presidente Antônio Carlos (Teófilo Otoni, Minas Gerais) os tubos foram unificados para dupla filtragem por centrifugação.…”

Section: Methodsunclassified

“…A diferença entre as prevalências observadas entre as etnias pode ser devida às diferentes técnicas utilizadas. Ao contrário das técnicas de sedimentação espontânea comumente utilizadas em inquéritos coprológicos, neste estudo utilizamos uma técnica que associa centrifugação, filtração e sedimentação e que apresenta alta sensibilidade na detecção de cistos de protozoários 16 . Na medida em que esses protozoários lesam a mucosa intestinal, provocando além da dor abdominal, diarreia e má-absorção, não deveriam ser negligenciados, devido não só ao risco de formas invasivas intestinais e extraintestinais de amebíase 7 , mas também frente ao risco de piorar o quadro nutricional dessa população indígena, que convive com o flagelo da desnutrição.…”

Section: Methodsunclassified

Prevalência de parasitos intestinais na comunidade indígena Maxakali, Minas Gerais, Brasil, 2009

Assis¹,

Olivieria²,

Moreira³

et al. 2013

Cad. Saúde Pública

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A comparative study of the TF-Test®, Kato-Katz, Hoffman-Pons-Janer, Willis and Baermann-Moraes coprologic methods for the detection of human parasitosis

Cited by 69 publications

References 9 publications

Strongyloides Stercoralis and Its Risk Factors: An Experience at a Tertiary Care Hospital

Strongyloides Stercoralis and Its Risk Factors: An Experience at a Tertiary Care Hospital

Reducing Occurrence of Giardia duodenalis in Children Living in Semiarid Regions: Impact of a Large Scale Rainwater Harvesting Initiative

Prevalência de parasitos intestinais na comunidade indígena Maxakali, Minas Gerais, Brasil, 2009

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