A comparative study of the fine structure of the trophoblast in several hemochorial placentas

Enders, Allen C.

doi:10.1002/aja.1001160103

Cited by 411 publications

(171 citation statements)

References 20 publications

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“…1), formed primarily of trophoblast, which are easily distinguished from each other using routine histological methods (Enders, 1965 ;Stewart, 1984). The giant cell trophoblast zone lies adjacent to maternal decidua.…”

mentioning

confidence: 99%

Lectin binding characteristics of mouse placental cells

2000

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The lectin binding characteristics of mouse placental cells were examined. Wax embedded tissue sections of placentae from d 14 pregnant mice were stained with 26 lectins, with a wide range of sugar specificities. Cell cultures prepared from d 14 mouse placentae and cultured for 24 h were stained with 7 of the lectins to determine if they could be used as markers for the different trophoblast cells in culture. In tissue sections all placental cell populations bound lectin but no lectin bound specifically to any single trophoblast population. All the lectins which bound to layer 1 cytotrophoblast lining the maternal blood spaces of the labyrinthine placenta also bound to the fetal endothelium of the labyrinthine placenta. Binding of lectin appeared strongest on the adluminal membrane of these cell populations suggesting a role for the carbohydrate moieties in nutrient transfer. Few lectins bound to junctional zone trophoblast. Overall, the binding of lectin to cultured cells did not correlate exactly with lectin binding to the cell populations in tissue sections. The value of lectins as markers for placental cells in culture was therefore found to be limited. Our findings indicate that carbohydrate expression by at least some placental cells may vary in culture from that expressed by the cells in vivo with obvious concerns for the validity of functional in vitro studies.

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confidence: 99%

Lectin binding characteristics of mouse placental cells

2000

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“…With the use of all three assays, it was found that in the pUPD12 junctional zone, ''glycogen'' cells are more abundant than normal at E13.5 and E15.5 (data not shown), and their dramatic -t3), the fetal capillary endothelium (fc), and its associated basement membrane (bm) in the normal feto-maternal interface (e) as shown previously (25,26). The outermost trophoblast layer (t3), which is cytotrophoblastic (25), is loosely attached to the middle layer (t2), which is syncytial (25). Note that the t2 layer has many vacuole-like spaces.…”

Section: Resultsmentioning

confidence: 80%

“…Note that the t2 layer has many vacuole-like spaces. These were shown to be due to the irregular invaginations of its maternal surface and are in direct contact with maternal blood plasma via the pores present in the t3 layer (25). ( f and h) Note that in the pUPD12 labyrinthine interfaces, the acellular discontinuity (asterisks) is between the innermost trophoblast layer (t1) and the basement membrane (bm).…”

Section: Resultsmentioning

confidence: 99%

“…The labyrinth is the zone where fetal blood and maternal blood circulate within fetal capillaries and maternal blood spaces, respectively, for physiological exchange through zygote-derived feto-maternal interfaces (1,2,(24)(25)(26). These labyrinthine interfaces consist of the endothelial cells of fetal capillaries, their associated basement membrane, and a trilaminar layer of trophoblast cells that directly lines the maternal blood spaces (25,26) (Fig.…”

Section: Resultsmentioning

confidence: 99%

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Roles for genomic imprinting and the zygotic genome in placental development

Georgiades

Watkins

Burton

et al. 2001

Proc. Natl. Acad. Sci. U.S.A.

130

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The placenta contains several types of feto-maternal interfaces where zygote-derived cells interact with maternal cells or maternal blood for the promotion of fetal growth and viability. The genetic factors regulating the interactions between different cell types within fetomaternal interfaces and the relative contributions of the maternal and zygotic genomes are poorly understood. Genomic imprinting, the epigenetic process responsible for parental origin-dependent functional differences between homologous chromosomes, has been proposed to contribute to these events. Previous studies showed that mouse conceptuses with an absence of imprinted differences between the two copies of chromosome 12 (upon paternal inheritance of both copies) die late in gestation and have a variety of defects, including placentomegaly. Here we examined the role of chromosome 12 imprinting in these placentae in more detail. We show that the spatial interactions between different cell types within fetomaternal interfaces are defective and identify abnormal behaviors in both zygote-derived and maternal cells that are attributed to the genome of the zygote but not the mother. These include compromised invasion of the maternal decidualized endometrium and the central maternal artery situated within it by zygote-derived trophoblast, abnormalities in the wall of the central maternal artery, and defects within the zygote-derived cellular layer of the labyrinth, which is in direct contact with maternal blood. These findings demonstrate multiple roles for chromosome 12 imprinting in the placenta that have not previously been associated with imprinting effects. They provide insights into the function of imprinting in placental development and have evolutionary and clinical implications.

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“…The structure of the placental membrane separating the maternal and foetal blood in the guinea-pig is similar to that in the human (Enders, 1965). As an experimental animal, the guinea-pig is comparatively cheap and readily available in the pregnant state, though the gestation is 67 days.…”

Section: Department Of Pharmacology University College Londonmentioning

confidence: 81%

Proceedings of the British Pharmacological Society

1973

British J Pharmacology

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A comparative study of the fine structure of the trophoblast in several hemochorial placentas

Cited by 411 publications

References 20 publications

Lectin binding characteristics of mouse placental cells

Lectin binding characteristics of mouse placental cells

Roles for genomic imprinting and the zygotic genome in placental development

Proceedings of the British Pharmacological Society

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