A comparative study of three different gene expression analysis methods

Choe, Jae Young; Han, Hyung Soo; Lee, Seon Duk; Lee, Hanna; Lee, Dong‐Eun; Ahn, Jae Yun; Ryoo, Hyun Wook; Seo, Kang Suk; Kim, Jong Kun

doi:10.3233/thc-170967

Cited by 3 publications

(1 citation statement)

References 15 publications

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“…LAMP assay kits are commercially available, but almost all of them use LAMP for detection as opposed to reliable quantification directly from the sample 11 , 12 , 19 – 27 . There have been some attempts at reliable quantification using Reverse transcription- Loop-mediated isothermal amplification (RT-LAMP), which has advantages over Reverse transcription-polymerase chain reaction (RT-PCR), such as simpler instrumentation and faster reaction times 7 , 28 , 29 . However, it has gained less popularity for quantification as RT-PCR.…”

Section: Introductionmentioning

confidence: 99%

Quantitative mRNA expression measurement at home

Pandey,

McCoy,

Stobdan

et al. 2024

Sci Rep

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AbstractmRNA measurement is dominated by RT-PCR, which requires expensive laboratory equipment and personnel with advanced degrees. Loop-mediated isothermal amplification (LAMP) is a versatile technique for detecting target DNA and RNA. The sensitivity of LAMP in early reports has been below that of the standard RT-PCR tests. Here, we report the use of a fluorescence-based RT-LAMP protocol to measure CDX2 expression patterns, which match extremely well to the standards of sophisticated RT-PCR techniques (r = 0.99, p < 0.001). The assay works on diverse sample types such as cDNA, mRNA, and direct tissue sample testing in 25 min compared to more than 3 h for RT-PCR. We have developed a new protocol for designing RT-LAMP primers that reduce false positives due to self-amplification and improve quantification. A simple device with a 3D-printed box enables the measurement of mRNA expression at home, outdoors, and point-of-care setting.

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