A comparative study on cannabidiol-induced apoptosis in murine thymocytes and EL-4 thymoma cells

Lee, Chi-Ya; Wey, Shiaw-Pyng; Liao, Mei-Hsiu; Hsu, Wei‐Lun; Wu, Hsin-Ying; Jan, Tong-Rong

doi:10.1016/j.intimp.2008.01.018

Cited by 76 publications

(61 citation statements)

References 30 publications

(72 reference statements)

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“…For in vitro studies investigating the direct effect of ANE exposure on thymocytes, a set of naive mice had their thymus isolated aseptically and made into single cell suspensions as described previously (Lee et al, 2008;Wang et al, 2007). The thymocytes were then cultured in RPMI 1640 medium supplemented with 100 U penicillin/ml, 100 mg streptomycin/ml, and 5% heat-inactivated FBS.…”

Section: Animals and Thymocytes Culturesmentioning

confidence: 99%

Impairment of thymocyte function via induction of apoptosis by areca nut extract

Lee

Lin

Liu

et al. 2013

Journal of Immunotoxicology

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Areca quid (AQ) chewing is a popular oral habit, especially in Southeast Asia cultures, in which children may be engaged in the addictive habit early in their lives. Extracts of areca nuts, the main component of AQ, have been shown to affect the functionality of T-cells. However, the potential influence of ANE on the development of T-cells is unknown. This study, therefore, investigated the impact of areca nut extracts (ANE) on thymocytes and the potential mechanisms of action. Mice administered intraperitoneally with ANE at 1, 5, or 25 mg/kg daily for 5 days showed significant dose-dependent reductions in thymocyte viability. A marked decrease in the total number of thymocytes and the proportion of thymic CD4 þ CD8þ cells was observed in the 25 mg ANE/kg-treated mice, whereas the proportion of CD4 and CD8 single positive and CD4À CD8 À cells was significantly increased. Further examination on the functionality of thymocytes showed that ANE suppress IL-2 production both ex vivo and in vitro. These results suggest that ANE may attenuate the development and functionality of thymic T-cells. ANE also directly induced apoptosis in thymic T-cells through activation of casapase-3 and apoptosis inducing factor (AIF). Collectively, the data suggested that the thymus is a sensitive target to ANE. Early exposure to ANE may interfere with the development and functionality of thymic T-cells.

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Section: Animals and Thymocytes Culturesmentioning

confidence: 99%

Impairment of thymocyte function via induction of apoptosis by areca nut extract

Lee

Lin

Liu

et al. 2013

Journal of Immunotoxicology

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“…Another possible mechanism that may account for the direct suppression of IFN-γ by CBD is the induction of apoptosis, as we previously reported that CBD ≥4 µmol/L caused a direct pro-apoptotic effect in naive lymphocytes [11,14] . However, the current study showed that CBD in a non-apoptotic concentration range (1-4 µmol/L) was effective to suppress IFN-γ, but did not affect OVA-induced IL-4 expression and the splenocyte metabolic activity.…”

Section: Discussionmentioning

confidence: 94%

Cannabidiol attenuates delayed-type hypersensitivity reactions via suppressing T-cell and macrophage reactivity

Liu¹,

Huang

et al. 2010

Acta Pharmacol Sin

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Aim: To investigate the effects of cannabidiol (CBD) on delayed-type hypersensitivity (DTH) reactions and antigen-induced T-cell cytokine expression. Methods: DTH was induced by subcutaneous ovalbumin (OVA) challenge to the footpads of mice sensitized with OVA. Inflammatory reactions were measured by footpad swelling and histological analysis. Antigen-induced cytokine expression by OVA-primed splenocytes was measured using ELISA and RT-PCR. Results: CBD (1-10 mg/kg) administration, in a dose-dependent fashion, significantly attenuated inflammatory reactions associated with DTH in the footpads of mice sensitized and challenged with OVA. Histological examination revealed that CBD suppressed the infiltration of T cells and macrophages, and the expression of interferon (IFN)-γ and tumor necrosis factor-α, two pro-inflammatory cytokines implicated in DTH in the inflammatory site. In contrast, the expression of interleukin (IL)-10 in the footpads was enhanced by CBD administration. In addition, CBD at concentrations devoid of cytotoxic effects (1-4 µmol/L) attenuated OVA-induced IFN-γ production by OVA-primed splenocytes, whereas IL-4 was unaffected. Conclusion: CBD curbs DTH reactions via suppressing the infiltration and functional activity of T cells and macrophages in the inflammatory site, suggesting a therapeutic potential for CBD for the treatment of type IV hypersensitivity.

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“…w białaczce, raku piersi (MCF7) i komórkach glioma [54]. Spostrzeżenie to może sugerować, że w guzach wrażliwych CBD może być czynnikiem antyrakowym [55].…”

Section: Spostrzeżenia Kliniczneunclassified

Current status and prospects for cannabinoids ("medical marihuana") used in children therapy. Review of opinions and standpoints

Wachowiak¹,

Krawczyński²,

Steinborn³

2016

Child Neurology

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A comparative study on cannabidiol-induced apoptosis in murine thymocytes and EL-4 thymoma cells

Cited by 76 publications

References 30 publications

Impairment of thymocyte function via induction of apoptosis by areca nut extract

Impairment of thymocyte function via induction of apoptosis by areca nut extract

Cannabidiol attenuates delayed-type hypersensitivity reactions via suppressing T-cell and macrophage reactivity

Current status and prospects for cannabinoids ("medical marihuana") used in children therapy. Review of opinions and standpoints

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