A Comparative Study on the Production of Galacto-oligosaccharide from Whey Permeate in Recycle Membrane Reactor and in Enzymatic Batch Reactor

Abstract

The present study primarily is an attempt to establish a technique on the disposal scheme for the wastewater coming out as an effluent from milk industries with proper environmental guides and regulations. Apart from the wastewater treatment methodology development, the present work mainly focuses on the attainment of high value product, such as the production of galactosyl-oligosaccharide (GOS) in batch reactor and as well as in recycle membrane reactor after hydrolyzing recovered lactose from the whey stream with -galactosidase enzyme originated, from Bacillus circulans. Around 77-78% purity of this GOS was achieved with threestep membrane separation techniques: the first one was the ultrafiltration followed by diafiltration equipped nanofiltration. In recycle membrane reactor, GOS production was found to be 33% higher than that in the batch reactor, and monosaccharide concentration was 78% lower with 23% remaining enzyme activity after 18,000 s of reaction time.

Improvement of thermal stability of β-galactosidase from Bacillus circulans by multipoint covalent immobilization in hierarchical macro-mesoporous silica

“…GOS are an important class of food-grade oligosaccharides, which are considered prebiotics [2]. The enzyme catalyzed reaction of GOS synthesis from lactose is under kinetic control, therefore it can be modulated by changes in lactose concentration [3], pH [4] and temperature of the reaction medium [5].…”

Microbial Glycosidases for Nondigestible Oligosaccharides Production

“…The production of GOs from milk whey using a two-dimensional packed bed bioreactor yielded 97% [13], while a yield of 29.9% of GOs with a concentration of (119.8 mg/mL) was achieved using cheese whey as substrate in a 4 h process [14]. When whey permeate was used as substrate in a membrane reactor system, a mixture of GOs with 77-78% of purity was produced [15]. A high lactose conversion was achieved (70-80%), when using whey as a substrate in the production of GOs, yielding 10-20% of total sugars and producing oligomers with DP3, DP4, and DP5 [16].…”

Production of β-galactosidase in a Batch Bioreactor Using Whey through Microbial Route – Characterization of Isolate and Reactor Model

“…Centrifugation was done at 10,000 rpm and 4 o C for 15 min. The temperature of the HPLC column was maintained at 15 o C. Acetonitrile 75% (v/v) was used as the mobile phase at a flow rate 1.67×10 -8 m 3 /s [18].…”