A comparative synthesis of transcriptomic analyses reveals major differences between WSSV-susceptible Litopenaeus vannamei and WSSV-refractory Macrobrachium rosenbergii

Peruzza, Luca; Thamizhvanan, S; Vimal, S.; Kumar, Karthik; Shekhar, M. S.; Smith, Valerie J.; Hauton, Chris; Vijayan, K.K.; Hameed, A.S. Sahul

doi:10.1016/j.dci.2019.103564

Cited by 28 publications

(12 citation statements)

References 66 publications

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“…However, according to our data, pro-resilin, cuticle proteins, hemolymph clottable, proclotting enzyme, and many HSPs genes differentially expressed in unhealthy condition (Figure 4) cannot be pointed out as good candidates in WSSV control since they were up-regulated in unhealthy infected shrimps with strong clinical signs. In addition, the cuticle, chitinase, and pro-resilin genes strongly up-regulated in unhealthy shrimps suggest a inhibition in molt process, with the majority of energy being required for immune defense [85].…”

Section: Discussionmentioning

confidence: 99%

Shedding the Light on Litopenaeus vannamei Differential Muscle and Hepatopancreas Immune Responses in White Spot Syndrome Virus (WSSV) Exposure

Santos

Andrade

Fernandes

et al. 2020

Genes

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White Spot Syndrome Virus (WSSV) is one of the main threats to farming Litopenaeus vannamei, the most important crustacean commercialized in aquaculture worldwide. Here, we performed RNA-seq analyses in hepatopancreas and muscle from WSSV-negative (healthy) and WSSV-positive (unhealthy) L. vannamei, previously exposed to the virus, to obtain new insights about the molecular basis of resistance to WSSV. We detected 71% of our reads mapped against the recently described L. vannamei genome. This is the first report mapping RNA-seq transcripts from shrimps exposed to WSSV against the species reference genome. Differentially expressed gene (DEG) analyses were performed for four independent comparisons, and 13,338 DEGs were identified. When the redundancies and isoforms were disregarded, we observed 8351 and 6514 DEGs, respectively. Interestingly, after crossing the data, we detected a common set of DEGs for hepatopancreas and healthy shrimps, as well as another one for muscle and unhealthy shrimps. Our findings indicate that genes related to apoptosis, melanization, and the Imd pathway are likely to be involved in response to WSSV, offering knowledge about WSSV defense in shrimps exposed to the virus but not infected. These data present potential to be applied in further genetic studies in penaeids and other farmed shrimp species.

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Section: Discussionmentioning

confidence: 99%

Shedding the Light on Litopenaeus vannamei Differential Muscle and Hepatopancreas Immune Responses in White Spot Syndrome Virus (WSSV) Exposure

Santos

Andrade

Fernandes

et al. 2020

Genes

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“…More than forty species of arthropods have been reported as hosts or carriers for WSSV either from culture facilities, the wild or experimental infection. WSSV caused 100% mortality in marine shrimp and freshwater crab but not in M. rosenbergii which is found to be tolerant to WSSV (Sahul Hameed et al., 1998, 2000; Peruzza et al., 2020; Sarathi et al., 2008). Sarathi et al.…”

Section: Discussionmentioning

confidence: 99%

“…This might be due to the activation of the components of the clotting cascade in WSSV‐injected M. rosenbergii (Cao et al., 2017; Rao et al., 2016) and suppression of clotting cascade components in WSSV‐infected susceptible shrimp species (Goncalves et al., 2014; Shi et al., 2018). Likewise, other immunological parameters such as prophenoloxidase (proPO), antioxidant enzymes and antimicrobial peptides were reported to have been activated in WSSV‐injected M. rosenbergii (Cao et al., 2017; Peruzza et al., 2020; Rao et al., 2016; Sarathi et al., 2008) and suppressed in susceptible shrimp species after WSSV infection (Kulkarni et al., 2014; Peruzza et al., 2020; Zhang et al., 2018). In our previous study, we found that nine differentially expressed immune genes were up‐regulated in WSSV‐injected M. rosenbergii (refractory species) and down‐regulated in.…”

Section: Introductionmentioning

confidence: 99%

“…In our previous study, we found that nine differentially expressed immune genes were up‐regulated in WSSV‐injected M. rosenbergii (refractory species) and down‐regulated in. L. vannamei (susceptible species) after WSSV infection (Peruzza et al., 2020). The nine differentially expressed genes (DEGs) were sialin transporter 1, trehalose transporter 1, two C‐type lectins, phenoloxidase 2, formyltetrahydrofolate dehydrogenase, sorbitol dehydrogenase, sulfotransferase and acetylcholinesterase (Peruzza et al., 2020).…”

Section: Introductionmentioning

confidence: 99%

“…L. vannamei (susceptible species) after WSSV infection (Peruzza et al., 2020). The nine differentially expressed genes (DEGs) were sialin transporter 1, trehalose transporter 1, two C‐type lectins, phenoloxidase 2, formyltetrahydrofolate dehydrogenase, sorbitol dehydrogenase, sulfotransferase and acetylcholinesterase (Peruzza et al., 2020). In the present study, an attempt was made to determine the role of prophenoloxidase in developing resistance in M. rosenbergii against WSSV by silencing using dsRNA specific to proPO gene.…”

Section: Introductionmentioning

confidence: 99%

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Silencing of prophenoloxidase (proPO) gene in freshwater prawn, Macrobrachium rosenbergii, makes them susceptible to white spot syndrome virus (WSSV)

Thamizhvanan¹,

Ahmed²,

Kumar³

et al. 2020

Journal of Fish Diseases

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Prophenoloxidase (proPO) is very important to protect the invertebrates from microbial infections. Our previous studies revealed that proPO was up‐regulated in WSSV‐injected Macrobrachium rosenbergii and is responsible for protecting M. rosenbergii from WSSV. In order to prove this mechanism, an attempt was made in the present study to silence the proPO gene in freshwater prawn by injection of dsRNA‐proPO followed by WSSV challenge. Two partial fragments of proPO with the size of 251 and 331 bp were used to synthesize dsRNA using LITMUS38i vector and E. coli. The bacterially synthesized dsRNA‐proPO was used to silence proPO gene to determine its involvement in developing resistance in prawn against WSSV. In proPO gene‐silenced prawn, 100% mortality was observed after WSSV challenge whereas no mortality was observed in prawn injected with WSSV alone. The WSSV infection in gene‐silenced prawn was confirmed by PCR, and its propagation was quantified by ELISA and real‐time PCR at different time intervals. Real‐time PCR assay revealed a significant reduction in the expression of proPO gene in WSSV‐challenged proPO‐silenced prawn when compared to normal prawn. Level of proPO was reduced significantly in the haemolymph of proPO‐silenced prawn when compared to prawn injected with PBS.

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Oral administration of Zingiber officinale and Aegle marmelos extracts enhances growth and immune functions of the shrimp Penaeus monodon against the white spot syndrome virus (WSSV)

et al. 2023

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A comparative synthesis of transcriptomic analyses reveals major differences between WSSV-susceptible Litopenaeus vannamei and WSSV-refractory Macrobrachium rosenbergii

Cited by 28 publications

References 66 publications

Shedding the Light on Litopenaeus vannamei Differential Muscle and Hepatopancreas Immune Responses in White Spot Syndrome Virus (WSSV) Exposure

Shedding the Light on Litopenaeus vannamei Differential Muscle and Hepatopancreas Immune Responses in White Spot Syndrome Virus (WSSV) Exposure

Silencing of prophenoloxidase (proPO) gene in freshwater prawn, Macrobrachium rosenbergii, makes them susceptible to white spot syndrome virus (WSSV)

Oral administration of Zingiber officinale and Aegle marmelos extracts enhances growth and immune functions of the shrimp Penaeus monodon against the white spot syndrome virus (WSSV)

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