A comparison between the 2N and 4N HCl acid-insoluble ash methods for digestibility trials in horses

Bergero, Domenico; Préfontaine, C.; Miraglia, N.; Peiretti, P. G.

doi:10.1017/s1751731109990656

Cited by 17 publications

(6 citation statements)

References 16 publications

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“…There are several methods for determining the apparent digestibility of feeds and feed rations in horses (Strakova and Suchy 2013). Total collection AST = aspartate aminotransferase; ALT = alanine aminotransferase; ALP = alkaline phosphatase a,b values followed by different superscripts differ within a row at P < 0.05 https://doi.org/10.17221/181/2019-CJAS of faeces is considered to be the most accurate of them (Bergero et al 2009). Digestibility coefficients obtained by TFC (Table 3) in E1 group compared to C group (feed rations enriched with 200 g of DGP) indicate a tendency (P < 0.1) of higher digestibility of DM, OM and CP (P > 0.05).…”

Section: Resultsmentioning

confidence: 99%

The effects of dried grape pomace supplementation on biochemical blood serum indicators and digestibility of nutrients in horses

Kolláthová¹,

Gálik²,

Halo³

et al. 2020

Czech J. Anim. Sci.

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Twelve adult Slovak warmblood sport horses were used to study the effect of dried grape pomace (DGP) on health through blood serum biochemical indicators, and on apparent total tract digestibility of dry matter (DM), organic matter (OM), crude protein (CP), acid detergent fibre (ADF) and neutral detergent fibre (NDF). The digestibility analysis was carried out by two in vivo methods, total faeces collection (TFC) and using lignin as a marker (ADL). Animals were divided into 3 groups: control group (C, without supplementation), experimental group 1 (E1, feed rations + 200 g of DGP) and experimental group 2 (E2, feed rations + 400 g of DGP). In animals, no health problems were detected during the trial. Of the blood serum indicators, only the concentrations of potassium (increase in E2 group compared to C group) and alanine aminotransferase (decrease in E2 group in comparison with E1 and C group) were affected (P < 0.05). The ADL method resulted in underestimated digestibility coefficients due to low recovery rates of lignin (less than 90%) in C group and E1 group. According to TFC, in E1 group higher digestibility coefficients were detected for DM, OM and CP (P > 0.05) compared to C group. However, in E2 group lower digestibility of all the studied nutrients was found (P > 0.05) in comparison with C group and E1 group. These results suggest that DGP could be used in horse diets up to 200 g without negative effect on their health and for a possible digestibility improvement of some nutrients.

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Section: Resultsmentioning

confidence: 99%

The effects of dried grape pomace supplementation on biochemical blood serum indicators and digestibility of nutrients in horses

Kolláthová¹,

Gálik²,

Halo³

et al. 2020

Czech J. Anim. Sci.

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“…The neutral detergent fiber (NDF, assayed without alpha-amylase), acid detergent fiber (ADF) and acid detergent lignin (ADL) contents were analyzed by the method reported by Van Soest et al [34]. Acid-insoluble ash was determined by incineration for 3 h at 550 • C according to the method described by Bergero et al [35].…”

Section: Chemical Analysesmentioning

confidence: 99%

Effects of Different Cutting Stages and Additives on the Fermentation Quality and Microbial Community of Sudangrass (Sorghum sudanense Stapf.) Silages

Yu,

Li,

Zhang

et al. 2023

Fermentation

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(1) Background: Previous studies have indicated that ferulic acid esterase (FAE), cellulase and xylanase have synergistic effects in lignocellulose degradation, and the cutting stage has a major impact on silages. Whether these additives affect the silages at different cutting stages is unclear. (2) Methods: Sudangrass height at the tested cutting stages was 1.8 m (S1) and 2.0 m (S2). The silage from the two cutting stages was treated with FAE-producing Lactobacillus plantarum (LP), cellulase and xylanase (CX) and a combination of LP and CX (LP+CX) for 30 and 60 days. (3) Results: Compared with CK, adding LP+CX significantly decreased the pH and the content of neutral detergent fiber (NDF) and acidic detergent fiber (ADF) (p < 0.05) and increased the lactic acid (LA) concentration (p < 0.05), dry matter (DM) content and crude protein content. Adding LP+CX effectively degraded lignocellulose in sudangrass, and the NDF and ADF degradation rates at the two stages were all more than 30%. In comparison, cutting at the S2 stage led to a lower pH and higher LA and DM contents (p < 0.05). Additives and the cutting stage exerted a strong effect on the silage microbial community, and Firmicutes and Lactiplantibacillus became the most dominant bacterial phyla and genera, especially at the S2 stage. (4) Conclusions: The results suggest that FAE-producing L. plantarum, cellulase and xylanase had synergistic effects on sudangrass silages, especially at the S2 stage, and their use can thus serve as an efficient method for ensiling.

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“…Acid-insoluble ash in diets and feces was determined by the method of Bergero et al [ 28 ]. Such a method allows one to determine the content of mineral substances insoluble in hydrochloric acid.…”

Section: Methodsmentioning

confidence: 99%

Forage:Concentrate Ratio Effects on In Vivo Digestibility and In Vitro Degradability of Horse’s Diet

Zicarelli

Tudisco

Lotito

et al. 2023

Animals

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Determination of digestibility represents the first step for the evaluation of the net energy content of feed for livestock animals. The aim of this study was to evaluate the in vivo digestibility and in vitro degradability of five diets characterized by different forage/concentrate ratios (F:C) in horses. The in vitro degradability was determined by the Gas Production Technique (GPT), using as an inoculum source the feces of the same subjects used for the in vivo test. Five diets consisting of poliphyte hay, straw and grains of barley and oats with a different F:C ratio [90/10 (Diet 1); 78/22 (Diet 2); 68/32 (Diet 3); 60/40 (Diet 4); 50/50 (Diet 5) were formulated and administered in succession, starting with Diet 1. In the in vivo results, no significant differences emerged, despite the different F:C content. In in vitro fermentation, four diets out of the five (2, 3, 4, 5) presented a similar trend of the curve of gas production, showing good activity of the fecal micro population during the first hours of incubation. An important correlation between gas and Volatile Fatty Acid (VFA) were found, suggesting that the processes linked to the micro population deriving from the horse’s caecum follow metabolic pathways whose products can be modeled in the same way as for the rumen. The GPT could represent the correct method for studying the nutritional characteristics of feed for horses, using feces as the source of inoculum, even if further investigations must be performed to improve the technique.

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A comparison between the 2N and 4N HCl acid-insoluble ash methods for digestibility trials in horses

Cited by 17 publications

References 16 publications

The effects of dried grape pomace supplementation on biochemical blood serum indicators and digestibility of nutrients in horses

The effects of dried grape pomace supplementation on biochemical blood serum indicators and digestibility of nutrients in horses

Effects of Different Cutting Stages and Additives on the Fermentation Quality and Microbial Community of Sudangrass (Sorghum sudanense Stapf.) Silages

Forage:Concentrate Ratio Effects on In Vivo Digestibility and In Vitro Degradability of Horse’s Diet

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