A comparison of cationic and anionic intercalators for the electrochemical transduction of DNA hybridization via long range electron transfer

Wong, Emily

doi:10.1016/s1388-2481(04)00110-9

Cited by 3 publications

(4 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The relatively small currents observed in Experiments 3 and 4 compared with Experiment 1 implied that the non-specific adsorption of AQMS was negligible. The slight adsorption of AQMS onto DNA probe (ssDNA) was consistent with observation previously reported [ 41 ]. The highest current observed for the complementary DNA (Experiment 1) thus indicated hybrization where intercalation of AQMS had occurred in dsDNA formed on the microsphere surface.…”

Section: Resultssupporting

confidence: 91%

“…The optimum microbiosensor response ( Figure 9 ) is dependent on the temperature and reached at a shorter time span for higher hybridization temperatures. The length of DNA hybridization time obtained herein is similar to that reported elsewhere [ 41 ]. Even at room temperature of 25 °C, the current response of the microbiosensor is large enough for further biosensor studies.…”

Section: Resultssupporting

confidence: 88%

See 1 more Smart Citation

An Electrochemical DNA Microbiosensor Based on Succinimide-Modified Acrylic Microspheres

Ulianas

Heng

Hanifah

et al. 2012

Sensors

View full text Add to dashboard Cite

An electrochemical microbiosensor for DNA has been fabricated based on new acrylic microspheres modified with reactive N-acryloxysuccinimide (NAS) functional groups. Hydrophobic poly(n-butylacrylate-N-acryloxysuccinimide) microspheres were synthesized in an emulsion form with a simple one-step photopolymerization technique. Aminated DNA probe was attached to the succinimde functional group of the acrylic microspheres via covalent bonding. The hybridization of the immobilized DNA probe with the complementary DNA was studied by differential pulse voltametry using anthraquninone-2-sulfonic acid monohydrate sodium salt (AQMS) as the electroactive hybridization label. The influences of many factors such as duration of DNA probe immobilization and hybridization, pH, type of ions, buffer concentrations, ionic strength, operational temperature and non-complementary DNA on the biosensor performance were evaluated. Under optimized conditions, the DNA microbiosensor demonstrated a linear response range to target DNA over a wide concentration range of 1.0 × 10−16 and 1.0 × 10−8 M with a lower limit of detection (LOD) of 9.46 × 10−17 M (R2 = 0.97). This DNA microbiosensor showed good reproducibility with 2.84% RSD (relative standard deviation) (n = 3). Application of the NAS-modified acrylic microspheres in the construction of DNA microbiosensor had improved the overall analytical performance of the resultant DNA microbiosensor when compared with other reported DNA biosensors using other nano-materials for membranes and microspheres as DNA immobilization matrices.

show abstract

Section: Resultssupporting

confidence: 91%

Section: Resultssupporting

confidence: 88%

An Electrochemical DNA Microbiosensor Based on Succinimide-Modified Acrylic Microspheres

Ulianas

Heng

Hanifah

et al. 2012

Sensors

View full text Add to dashboard Cite

show abstract

“…Significant attention has also focused on improving the detection methods for DNA hybridization (Palecek, 2002). The hybridization event has been detected via electroactive or redox indicators such as metal coordination complexes or intercalating organic compounds (Peng et al, 2002;Wong et al, 2004;Meric et al, 2002;Ju et al, 2003;Babkina et al, 2004). Besides electrochemical detection, SPR has gained significant popularity in DNA sensing and other bioapplications.…”

Section: Biorecognition Elementsmentioning

confidence: 99%

Biosensor technology: Technology push versus market pull

Luong

Male

Glennon

2008

Biotechnology Advances

376

178

View full text Add to dashboard Cite

“…Finally, gold nanoparticles and 5' end -SH modified DNA (HS-DNA) as nucleotide probes were self-assembled onto the surface of Au electrode respectively. DNA hybridization was assessed with the changes in the voltammetric peak of an indicator of anthraquinone-2, 6-disulfonic acid (AQDS) [10] .This DNA immobilization strategy demonstrates that the DNA immobilized on self-assembled MWCNTs yields a higher response current than that without MWCNTs. The protocol for DNA probe immobilization and hybridization of probe with target were illustrated in Fig.…”

Section: Introductionmentioning

confidence: 99%

DNA Biosensors Based on Self-Assembled Multi-Walled Carbon Nanotubes

Pang

Zhang

Xiao

et al. 2009

AMR

View full text Add to dashboard Cite

A novel electrochemical DNA biosensor system based on multi-walled carbon nanotubes(MWCNTs) was presented in this paper. In order to make multi-walled carbon nanotubes being perpendicularly standing on Au electrode via Au-S chemical bonding, we cut multi-walled carbon nanotubes (1.5 m) into short pipes (200-300 nm). Then, the shortened nanotubes were further modified by thiolization reaction with cysteamine (NH2CH2CH2SH). The –SH modified MWCNTs, gold nanoparticles and 5’ end –SH modified DNA (HS-DNA) as nucleotide probes were self-assembled onto the surface of Au electrode respectively. The DNA biosensors based on self-assembled MWCNTs had a higher current response compared to those based on random MWCNTs. When the target DNA concentration was 4.0×10–9 mol/L to 1.2×10-8 mol/L, the cathodic peak current of Au electrode system with the AQDS as indicator was linearly related, and the detection limit was about 6.5×10-9 mol/L. In addition, the developed DNA biosensors also had a high selectivity of hybridization detection.

show abstract

A comparison of cationic and anionic intercalators for the electrochemical transduction of DNA hybridization via long range electron transfer

Cited by 3 publications

References 0 publications

An Electrochemical DNA Microbiosensor Based on Succinimide-Modified Acrylic Microspheres

An Electrochemical DNA Microbiosensor Based on Succinimide-Modified Acrylic Microspheres

Biosensor technology: Technology push versus market pull

DNA Biosensors Based on Self-Assembled Multi-Walled Carbon Nanotubes

Contact Info

Product

Resources

About