A comparison of fixation methods for SEM analysis of self-assembling peptide hydrogel nanoarchitecture

McFetridge, Meg L.; Kulkarni, Ketav; Hilsenstein, Volker; Borgo, Mark P. Del; Aguilar, Marie‐Isabel; Ricardo, Sharon D.

doi:10.1039/d2nr04598b

Cited by 9 publications

(5 citation statements)

References 40 publications

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“…The supramolecular fibrillar elements are also characterized by a right-handed twisting. This mutual entanglement of fibers is typically observed for peptide-based hydrogels [50].…”

Section: Sem Characterizationmentioning

confidence: 59%

Multicomponent Peptide-Based Hydrogels Containing Chemical Functional Groups as Innovative Platforms for Biotechnological Applications

Giordano,

Gallo,

Diaferia

et al. 2023

Gels

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Multicomponent hydrogels (HGs) based on ultrashort aromatic peptides have been exploited as biocompatible matrices for tissue engineering applications, the delivery of therapeutic and diagnostic agents, and the development of biosensors. Due to its capability to gel under physiological conditions of pH and ionic strength, the low molecular-weight Fmoc-FF (Nα-fluorenylmethoxycarbonyl-diphenylalanine) homodimer is one of the most studied hydrogelators. The introduction into the Fmoc-FF hydrogel of additional molecules like protein, organic compounds, or other peptide sequences often allows the generation of novel hydrogels with improved mechanical and functional properties. In this perspective, here we studied a library of novel multicomponent Fmoc-FF based hydrogels doped with different amounts of the tripeptide Fmoc-FFX (in which X= Cys, Ser, or Thr). The insertion of these tripeptides allows to obtain hydrogels functionalized with thiol or alcohol groups that can be used for their chemical post-derivatization with bioactive molecules of interest like diagnostic or biosensing agents. These novel multicomponent hydrogels share a similar peptide organization in their supramolecular matrix. The hydrogels’ biocompatibility, and their propensity to support adhesion, proliferation, and even cell differentiation, assessed in vitro on fibroblast cell lines, allows us to conclude that the hybrid hydrogels are not toxic and can potentially act as a scaffold and support for cell culture growth.

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“…The supramolecular fibrillar elements are also characterized by a right-handed twisting. This mutual entanglement of fibers is typically observed for peptide-based hydrogels [50].…”

Section: Sem Characterizationmentioning

confidence: 59%

Multicomponent Peptide-Based Hydrogels Containing Chemical Functional Groups as Innovative Platforms for Biotechnological Applications

Giordano,

Gallo,

Diaferia

et al. 2023

Gels

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show abstract

“…SEM demonstrated that the diluted gels exhibited a filamentary structure consisting of nanofibers with diameters of approximately 40-60 nm. These morphological features all indicated that multiple fibers with lengths greater than 600 nm were entangled with each other to produce a three-dimensional network structure, i.e., a macroscopic gel [30,31]. It was also observed that there was a dense interweaving of nanofibers in these undiluted three-dimensional networks, particularly in hydrogels at pH 5.0.…”

Section: Hydrogel Preparationmentioning

confidence: 93%

One-Step Construction of Tryptophan-Derived Small Molecule Hydrogels for Antibacterial Materials

Song

Zheng

et al. 2023

Molecules

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Amino acid-based hydrogels have received widespread attention because of their wide range of sources, biodegradability, and biocompatibility. Despite considerable progress, the development of such hydrogels has been limited by critical problems such as bacterial infection and complex preparation. Herein, by using the non-toxic gluconolactone (GDL) to adjust the pH of the solution to induce the rapid self-assembly of N-[(benzyloxy)carbonyl]-L-tryptophan (ZW) to form a three-dimensional (3D) gel network, we developed a stable and effective self-assembled small-molecule hydrogel. Characterization assays and molecular dynamics studies indicate that π–π stacking and hydrogen bonding are the main drivers of self-assembly between ZW molecules. In vitro experiments further confirmed this material’s sustained release properties, low cytotoxicity, and excellent antibacterial activity, particularly against Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus. This study provides a different and innovative perspective for the further development of antibacterial materials based on amino acid derivatives.

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“…1). 8,34,38,41 Cell culture Human bone marrow-derived mesenchymal stem cells (MSCs) purchased from the Tulane Center for Stem Cell Research and Regenerative Medicine (Tulane University, New Orleans, LA, USA) and characterised as previously reported. 46 MSCs were cultured in a media consisting of α-minimum essential media (αMEM; Invitrogen) supplemented with 16.5% foetal bovine serum (FBS; GIBCO), 2 mM L-glutamine, 100 U mL −1 penicillin and 100 μg mL −1 streptomycin (all from Invitrogen), according to the Tulane Center for Gene Therapy Protocol for expansion of Human MSCs.…”

Section: Methodsmentioning

confidence: 99%

“…Lipidated peptides with cell penetrating properties can be applied to direct peptide drugs, or other cargo, to specific subcellular regions, such as the mitochondrial membrane 28 in targeted cancer treatments. [29][30][31][32] Our laboratory has developed self-assembling peptide hydrogels for a range of applications including 2D cell culture, 8,9,[33][34][35][36][37][38] cellular guidance 36 and drug delivery. 9 Fibres are generated from the hierarchical self-assembly of N-acetylated β 3 -peptides 39,40 and functionalisation with a lipid promotes hydrogelation.…”

Section: Introductionmentioning

confidence: 99%

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Elucidating the cell penetrating properties of self-assembling β-peptides

McFetridge,

Kulkarni,

Lee

et al. 2023

Nanoscale

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A comparison of fixation methods for SEM analysis of self-assembling peptide hydrogel nanoarchitecture

Abstract: SEM preparation techniques for self-assembling peptide hydrogels can have dramatic influence on apparent structure.

Cited by 9 publications

References 40 publications

Multicomponent Peptide-Based Hydrogels Containing Chemical Functional Groups as Innovative Platforms for Biotechnological Applications

Multicomponent Peptide-Based Hydrogels Containing Chemical Functional Groups as Innovative Platforms for Biotechnological Applications

One-Step Construction of Tryptophan-Derived Small Molecule Hydrogels for Antibacterial Materials

Elucidating the cell penetrating properties of self-assembling β-peptides

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