A comparison of linear and branched polyethylenimine (PEI) with DCChol/DOPE liposomes for gene delivery to epithelial cells in vitro and in vivo

Wiseman, John; Goddard, Catharine A.; McLelland, Douglas; Colledge, William H

doi:10.1038/sj.gt.3302050

Cited by 195 publications

(150 citation statements)

References 31 publications

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“…Among them, L-PEI was chosen for its ability to lead to robust transgene expression in vivo following various administration routes. 2,3,[23][24][25][27][28][29] Moreover, it has been previously demonstrated that linear PEI is more efficient than the branched polymer in vitro and in vivo, 4,30,31 and Figure 4 b-Galactosidase activity in tumor nodes developed on the peritoneal wall of mice transfected with 100 or 250 mg DNA, evaluated 24 h after injection as described in Materials and methods.…”

Section: Discussionmentioning

confidence: 98%

Intraperitoneal linear polyethylenimine (L-PEI)-mediated gene delivery to ovarian carcinoma nodes in mice

Dutoit

Denoux

et al. 2005

Cancer Gene Ther

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Linear polyethylenimine (L-PEI) is an efficient transfection agent for ovarian carcinoma cells in vitro and ex vivo. In the present work, we go a step further and evaluate the efficacy of L-PEI in human ovarian tumor nodes developed in mice. PEI/DNA complexes were administered intraperitoneally instead of intravenously to avoid sequestering of complexes in the lung and liver and to allow transfection of nonvascularized tumor nodes. Plasmid biodistribution was studied by PCR and gene expression was characterized using complementary luciferase and b-galactosidase assays. Intraperitoneal (i.p.) injection of L-PEI/DNA complexes allowed the straightforward distribution of plasmid in the whole peritoneal cavity. Gene expression occurred in many organs, but tumor nodes appeared as preferential sites for transgene expression. The i.p. delivery route allowed repeated injections and administration of large amounts of DNA (up to 400 mg) without signs of toxicity, even for doses well beyond the intravenous lethal dose. Transgene expression was dose-dependent and transient. However, multiple injections allowed its persistence to increase. These results provide encouraging elements towards the development of PEI-based gene therapy protocols for the treatment of advanced stage ovarian carcinoma.

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Section: Discussionmentioning

confidence: 98%

Intraperitoneal linear polyethylenimine (L-PEI)-mediated gene delivery to ovarian carcinoma nodes in mice

Dutoit

Denoux

et al. 2005

Cancer Gene Ther

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“…[20] A number of studies have shown that, in selected cell lines, branched PEI generates stronger transgene expression that linear PEI. [18,31] In contrast, when PEI is injected intravenously or via the lungs, linear PEI, generates stronger transgene expression than branched PEI. [31,34] In vitro studies show that increasing the N:P ratio of the PEI-pDNA nanoparticles increases luciferase activity and toxicity.…”

Section: Discussionmentioning

confidence: 99%

“…[13,30,31] Several studies have shown that linear PEI generates stronger transgene expression in vivo when injected intravenously or administered via the lung in comparison to branched PEI at equivalent molecular weights. [24,[31][32][33][34][35] In contrast, other studies have shown that branched PEI generates stronger transgene expression than linear PEI in vitro in selected cell lines. [18] Some studies have shown that linear PEI is more efficient than branched PEI in vitro and in vivo.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of the transgene expression generated by branched and linear polyethylenimine-plasmid DNA nanoparticles in vitro and after intraperitoneal injection in vivo

Intra¹,

Salem²

2008

Journal of Controlled Release

125

119

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Polyethylenimine (PEI) is a cationic polymer that has shown significant potential for delivering genes in vitro and in vivo. Mixing cationic PEI with negatively charged plasmid DNA (pDNA) results in the spontaneous electrostatic formation of stable nanoparticle complexes. The structure of PEI can be branched or linear. In this study, we show that branched PEI has a stronger electrostatic interaction with pDNA than linear PEI, which accounts for greater compaction, higher zeta potentials and smaller nanoparticle sizes at equivalent pDNA concentrations. For both linear and branched PEI, increasing the concentration of pDNA mixed in the same volume and at the same nitrogen to phosphate (N:P) ratio results in larger average particle sizes. Increasing the N:P ratio increases luciferase activity generated by branched PEI-pDNA nanoparticles and linear PEI-pDNA nanoparticles in HEK293, COS7 and HeLa cell lines. Increasing the N:P ratio at which branched PEI-pDNA nanoparticles are prepared also increases luciferase expression in HepG2 cells but does not increase luciferase expression generated by linear PEI-pDNA nanoparticles. In all of the cell lines, branched PEI-pDNA nanoparticles prepared at N:P ratios of 10 and above generated significantly higher luciferase activity than linear PEI-pDNA nanoparticles. Luciferase activity was highest in the HEK293 cells and luciferase expression in each of the cell lines followed the order of HEK293

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“…[37] Cationic lipids are typically composed of a positively charged head group, a flexible linker group (e.g., an ester or ether), and two or more hydrophobic tail groups. [5] For improving transfection efficiency, cationic lipids are often mixed with helper lipids such as cholesterol or DOPE (1, 2-dioleyl-sn-glycerol-3-phosphoethanolamine) potentially promoting conversion of the lamellar lipoplex phase into a hexagonal structure.…”

Section: Lipid-based Non-viral Vectorsmentioning

confidence: 99%

Different strategies of gene delivery for treatment of cancer and other disorders

Agi

Mosaferi

Khatamsaz

et al. 2016

JST

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Gene therapy is the gene transfer into host cells for treatment of acquired and genetic disorders. For this purpose, there are a wide variety of gene delivery methods with special properties including viral and non-viral vectors. The non-viral methods use physical forces or chemical compounds (natural or synthetic) to transfer DNA into a cell. The efficiency of the non-viral gene therapy depends on conquering four different intra-and extra-cellular barriers such as cellular uptake, endosomal escape, nuclear entry, and gene expression. Among various gene carriers, some viral vectors such as Adenovirus, Lentivirus, Vaccinia as well as gene gun and lipofection achieved to clinical trials. In this mini-review, we briefly describe different approaches for gene delivery and their applications in various phases of clinical trials.

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A comparison of linear and branched polyethylenimine (PEI) with DCChol/DOPE liposomes for gene delivery to epithelial cells in vitro and in vivo

Cited by 195 publications

References 31 publications

Intraperitoneal linear polyethylenimine (L-PEI)-mediated gene delivery to ovarian carcinoma nodes in mice

Intraperitoneal linear polyethylenimine (L-PEI)-mediated gene delivery to ovarian carcinoma nodes in mice

Characterization of the transgene expression generated by branched and linear polyethylenimine-plasmid DNA nanoparticles in vitro and after intraperitoneal injection in vivo

Different strategies of gene delivery for treatment of cancer and other disorders

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