A comparison of methods for labelling DNA for use in the radioimmuno assay of DNA-antibodies

Avidity of antibodies in sle

“…It has been shown that traces of denatured DNA (0.1% of the total) may react in complement fixation. In chemically labeled IZ6I-DNA, the presence of protein traces, exposure to oxidizing agents, and high specific radioactivity resulting in damage to DNA may distort the results (17)(18)(19). In contrast, labeling the DNA by biologic incorporation of Y-iododeoxyuridine (IUdR) reduces or eliminates these difficulties and simultaneously provides the sensitivity and convenience of gamma isotope counting.…”