A comparison of the effects of Stevia extract and metformin on metabolic syndrome indices in rats fed with a high‐fat, high‐sucrose diet

Ranjbar, Sara; Nekooeian, Ali Akbar; Tanideh, Nader; Koohi-Hosseinabadi, Omid; Masoumi, Seyed Jalil; Amanat, Sasan; Azarpira, Negar; Monabati, Ahmad

doi:10.1111/jfbc.13242

Cited by 14 publications

(8 citation statements)

References 41 publications

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“…Extract against SKVO3 Cells. Cell cycle arrest caused by the control of essential proliferating proteins and stimulation of the apoptotic process are the two basic mechanisms underlying Stevia's cytotoxicity [20]. As a result, in this study, the following potential apoptotic…”

Section: Te Hypothesized Apoptotic Mechanisms Of the Steviamentioning

confidence: 88%

“…Additionally, Stevia may assist in lowering blood sugar, depriving tumor cells of a crucial source of energy. Consequently, tumor models in vivo and cancer cells showed cytotoxic and antiproliferative activities by Stevia plant derivatives [20]. Te anticancer efectiveness of the methanol Stevia extract against MCF7 cells was previously reported, with an IC 50 value of 228 µg/ml [91].…”

Section: Mtt and Ldh Cytotoxicity Assaysmentioning

confidence: 96%

“…S. rebaudiana hydromethanolic extract (SRHME) of plant leaves was prepared as reported earlier [20]. About 400 g of fnely ground Stevia leaves was immersed in solution containing 80 methanol : 20 distilled water for 72 h with constant shaking, and the resulting mixture was fltered to remove the extract-containing solvent from the crushed leaves.…”

Section: Preparation Of Hydromethanolic Extract Of Stevia Leavesmentioning

confidence: 99%

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Stevia rebaudiana Methanolic Leaf Extract in Egypt: Phytochemical Analysis, Antioxidant, Antilipid Peroxidation, Antihemolytic, Antimetastatic, and Anticancer Properties

Hanna

Osailan

Ahmed

2023

Journal of Food Biochemistry

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The phenolic and flavonoid content of the Stevia rebaudiana hydromethanolic extract (SRHME) was examined, and phytochemical identification using GC-mass spectrometry was achieved. Also, the antioxidant, antihemolytic, and antilipid peroxidation capabilities of the extract were assessed. The extract’s potential to induce apoptosis, inhibit cell proliferation, and reduce metastasis in SKVO3 cells was also checked. The findings of the GC-MS chromatogram demonstrated the existence of bioactive antioxidants and anticancer components in SRHME. Moreover, the extract demonstrated protection against cellular oxidative damage in human erythrocytes by preventing lipid peroxidation and hemolysis. Besides, SRHME demonstrated a selective cytotoxic effect with a strong IC50 value (17.5 µg/ml) on SKVO3 cells without any harmful effects on normal WI-38 cells using the MTT and LDH tests. Additionally, according to assays for wound healing and transwell chamber, the studied extract suppressed the ability of SKOV3 cells from migrating and invading, respectively. Also, the extract-treated SKVO3 cells showed rise in the percentage of apoptotic cells with a prominent comet nucleus, according to apoptotic assays in comparison to untreated cells. Furthermore, a flow cytometry analysis of SRHME-treated SKVO3 cells showed a halt in the S phase and an increase in sub-G1 apoptotic cells (25.44%). Also, the tested extract significantly decreased the levels of ROS in the treated cells, indicating that ROS was involved in the production of SKVO3 apoptosis. Lastly, SRHME strongly impacted the expression levels of proteins related to apoptosis, S-phase cell cycle arrest, and antimetastatic capacity in the treated SKVO3 cells.

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Section: Te Hypothesized Apoptotic Mechanisms Of the Steviamentioning

confidence: 88%

Section: Mtt and Ldh Cytotoxicity Assaysmentioning

confidence: 96%

Section: Preparation Of Hydromethanolic Extract Of Stevia Leavesmentioning

confidence: 99%

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Stevia rebaudiana Methanolic Leaf Extract in Egypt: Phytochemical Analysis, Antioxidant, Antilipid Peroxidation, Antihemolytic, Antimetastatic, and Anticancer Properties

Hanna

Osailan

Ahmed

2023

Journal of Food Biochemistry

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“…Noteworthy, the lipid-lowering effect of stevioside was abolished by silencing PPARa (Jia et al 2019), suggesting that its effects were mediated by activation of PPARa. Very recently, the effects of Stevia's hydroalcoholic extract (200 and 400 mg/kg) on metabolic syndrome indices were compared with those of 100 mg/kg metformin, in rats fed with a highfat, high-sucrose diet (Ranjbar et al 2020). Both treatments suppressed the adverse effects of the high fat and high sugar diet on the lipid profile, liver enzymes, antioxidant capacity, and histopathologic factors.…”

Section: Potential Use In Diabetes and Metabolic Syndromementioning

confidence: 99%

Potential medicinal effects and applications of stevia constituents

Talevi¹

2021

Phytochem Rev

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The application of stevia glycosides in the food industry has grown steadily in the last two decades. Besides the use of its constituents as natural sweeteners, increasing attention has been given to their potential health benefits and therapeutic applications. Whereas in some cases clinical studies have been realized (prominently, to investigate its hypoglycemic and antihypertensive activity) many of them have failed to cover the expectations from preclinical investigations. Here, we review current knowledge on stevia constituents' pharmacokinetics (both in rodents and in humans) and some of the most profusely investigated potential medical/health-related applications: i.e. diabetes and metabolic syndrome, hypertension, antimicrobial and anticancer activity. The outcome of available clinical studies and the perspectives of Stevia therapeutic uses are discussed in connection with pharmacokinetics knowledge.

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“…Metabolic syndrome is characterized by hyperglycemia, hyperlipidemia and central obesity, which increases the risk of various diseases including cardiovascular disease, diabetes, non-alcoholic fatty liver and cancer (Lent-Schochet et al, 2019). Latest epidemiological data show that the global prevalence of metabolic syndrome exceeds 20% (Tahereh et al, 2020). Due to the lack of potent pharmacotherapy, the rising prevalence of metabolic syndrome poses a huge threat to human health worldwide.…”

Section: Introductionmentioning

confidence: 99%

Kun-Dan Decoction Ameliorates Insulin Resistance by Activating AMPK/mTOR-Mediated Autophagy in High-Fat Diet-Fed Rats

Zeng

Feng

et al. 2021

Front. Pharmacol.

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Background: Metabolic syndrome is characterized by central obesity, hyperglycemia and hyperlipidemia. Insulin resistance is the leading risk factor for metabolic syndrome. Kun-Dan decoction (KD), a traditional Chinese medicine, has been applied to treat patients with metabolic syndrome for over ten years. It is increasingly recognized that autophagy deficiency is the key cause of metabolic syndrome. Therefore, we aimed to explore whether KD can activate autophagy to improve metabolic syndrome.Methods: Network pharmacology was used to explore the underlying mechanism of KD in the treatment of metabolic syndrome. The high-fat diet-fed rats and oleic acid-induced LO2 cells were employed in our study. Oral glucose tolerance test and insulin tolerance test, obesity and histological examination, serum cholesterol, triglyceride, low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), homeostasis model assessment of insulin resistance (HOMA-IR) and insulin sensitivity in high-fat diet-fed rats were analyzed. Furthermore, the protein expressions of adenosine 5'-monophosphate (AMP)-activated protein kinase (AMPK), phospho-AMPK, mammalian target of rapamycin (mTOR), phospho-mTOR, p62, autophagy related protein (Atg) 5, Atg7, Atg12, Atg13, Atg16L1 and microtubule-associated protein 1A/1B-light chain 3 (LC3)-Ⅱ/Ⅰ were examined in rats and LO2 cells. Moreover, autophagy activator rapamycin and inhibitor 3-methyladenine, and small interfering RNA against Atg7 were utilized to verify the role of autophagy in the treatment of metabolic syndrome by KD in oleic acid-induced LO2 cells.Results: Results from network pharmacology indicated that targeted insulin resistance might be the critical mechanism of KD in the treatment of metabolic syndrome. We found that KD significantly suppressed obesity, serum cholesterol, triglyceride and LDL-C levels and increased serum HDL-C level in high-fat diet-fed rats. Furthermore, KD enhanced insulin sensitivity and attenuated HOMA-IR in high-fat diet-fed rats. Western blot showed that KD could enhance autophagy to increase the insulin sensitivity of high-fat diet-fed rats and oleic acid-induced LO2 cells. Furthermore, 3-methyladenine and small interfering RNA against Atg7 could reverse the protective effect of KD on LO2 cells. However, rapamycin could cooperate with KD to enhance autophagic activation to increase insulin sensitivity in LO2 cells.Conclusion: The induction of autophagy may be the major mechanism for KD to improve insulin resistance and metabolic syndrome.

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A comparison of the effects of Stevia extract and metformin on metabolic syndrome indices in rats fed with a high‐fat, high‐sucrose diet

Cited by 14 publications

References 41 publications

Stevia rebaudiana Methanolic Leaf Extract in Egypt: Phytochemical Analysis, Antioxidant, Antilipid Peroxidation, Antihemolytic, Antimetastatic, and Anticancer Properties

Stevia rebaudiana Methanolic Leaf Extract in Egypt: Phytochemical Analysis, Antioxidant, Antilipid Peroxidation, Antihemolytic, Antimetastatic, and Anticancer Properties

Potential medicinal effects and applications of stevia constituents

Kun-Dan Decoction Ameliorates Insulin Resistance by Activating AMPK/mTOR-Mediated Autophagy in High-Fat Diet-Fed Rats

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