A comparison of two different cryoprotectants on post-thaw sperm parameters and fertilization success in tiger salamanders (Ambystoma tigrinum)

Chen, Devin M.; Lampert, Shaina S.; Chen, Li-Dunn; Burger, Isabella J.; Kouba, Carrie K.; Kouba, Andrew J.

doi:10.1016/j.cryobiol.2022.11.158

Cited by 2 publications

(3 citation statements)

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“…The overall average sperm concentration was 2.2 ± 3.5 x 10 6 sperm/mL, whereas the overall average sperm total motility was 45.7 ± 19.4%. Sperm was of high enough concentration to use for in-vitro fertilization, and we were able to cryopreserve several samples following the methods used in tiger salamanders [ 37 ].…”

Section: Resultsmentioning

confidence: 99%

Oral administration of GnRH via a cricket vehicle stimulates spermiation in tiger salamanders (Ambystoma tigrinum)

Chen,

Kouba

et al. 2024

PLoS ONE

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More than 50% of caudates are threatened with extinction and are in need of ex-situ breeding programs to support conservation efforts and species recovery. Unfortunately, many salamander populations under human care can experience reproductive failure, primarily due to missing environmental cues necessary for breeding. Assisted reproductive technologies (ARTs) are a useful suite of techniques for overcoming or bypassing these missing environmental cues to promote breeding. Exogenous hormones are used to stimulate natural breeding behaviors or gamete expression for in-vitro fertilization or biobanking and are typically administered intramuscularly in caudates. While effective, intramuscular injection is risky to perform in smaller-bodied animals, resulting in health and welfare risks. This research investigated the spermiation response to hormone administration through a non-invasive oral bioencapsulation route using the tiger salamander (Ambystoma tigrinum) as a model species. Male salamanders were randomly rotated six weeks apart through four treatments (n = 11 males/treatment) in which animals received a resolving dose of gonadotropin-releasing hormone (GnRH) as follows: (1) Prime-Only (0.0 μg/g); (2) Low (0.25 μg/g); (3) Medium (1.0 μg/g); and (4) High (2.0 μg/g). All males were given a GnRH priming dose (0.25 μg/g) 24 hours prior to the resolving dose. Exogenous hormone was delivered inside of a cricket (Gryllodes sigillatus) that was presented as a food item by tweezers. Sperm samples were collected at 1, 3, 6, 9, 12, and 24 hours after the resolving dose and analyzed for quantity and quality. For all treatments, sperm concentration was produced in an episodic pattern over time. The Prime-Only treatment had a lower (p < 0.05) percent of sperm exhibiting normal morphology compared to treatments utilizing a resolving dose of GnRH. Overall, oral administration of GnRH is a feasible route of inducing spermiation in salamanders, yielding sperm of sufficient quantity and quality for in-vitro fertilization and biobanking efforts.

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Section: Resultsmentioning

confidence: 99%

Oral administration of GnRH via a cricket vehicle stimulates spermiation in tiger salamanders (Ambystoma tigrinum)

Chen,

Kouba

et al. 2024

PLoS ONE

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“…Although this is an order of magnitude less, a concentration above 0.5 x 10 6 sperm/mL is suitable for the fertilization of eggs in this species. For example, 88 viable tiger salamander larvae were recently produced using cryopreserved sperm at a final concentration of 0.5 x 10 6 sperm/mL [38]. When…”

Section: Discussionmentioning

confidence: 99%

“…The overall average sperm concentration was 1.9 ± 1.2 x 10 6 sperm/mL, whereas the overall average sperm total motility was 46.3 ± 6.7%. Sperm was of high enough concentration to use for invitro fertilization, and we were able to cryopreserve several samples following the methods used in tiger salamanders [38].…”

Section: Case Studymentioning

confidence: 99%

Oral administration of GnRH via a cricket vehicle stimulates spermiation in tiger salamanders (Ambystoma tigrinum)

Chen

Kouba

et al. 2023

Preprint

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More than 50% of caudates are threatened with extinction and are in need ofex-situbreeding programs to support conservation efforts and species recovery. Unfortunately, many salamander populations under human care can experience reproductive failure, primarily due to missing environmental cues necessary for breeding. Assisted reproductive technologies (ARTs) are a useful suite of techniques for overcoming or bypassing these missing environmental cues to promote breeding. Exogenous hormones are used to stimulate natural breeding behaviors or gamete expression forin-vitrofertilization or biobanking and are typically administered intramuscularly in caudates. While effective, intramuscular injection is risky to perform in smaller-bodied animals, resulting in health and welfare risks. This research investigated the spermiation response to hormone administration through a non-invasive oral route using the tiger salamander (Ambystoma tigrinum) as a model species. Male salamanders were randomly rotated six weeks apart through four treatments (n = 11 males/treatment) in which animals received a resolving dose of gonadotropin-releasing hormone (GnRH) as follows: (1) Prime-Only (0.0 µg/g); (2) Low (0.25 µg/g); (3) Medium (1.0 µg/g); and (4) High (2.0 µg/g). All males were given a GnRH priming dose (0.25 µg/g) 24 hours prior to the resolving dose. Exogenous hormone was delivered inside of a cricket (Gryllodes sigillatus) that was presented as a food item by tweezers. Sperm samples were collected at 1, 3, 6, 9, 12, and 24 hours after the resolving dose and analyzed for quantity and quality. For all treatments, sperm concentration was produced in an episodic pattern over time. The Prime-Only treatment had a lower (p < 0.05) percent of sperm exhibiting normal morphology compared to treatments utilizing a resolving dose of GnRH. Overall, oral administration of GnRH is a feasible route of inducing spermiation in salamanders, yielding sperm of sufficient quantity and quality forin-vitrofertilization and biobanking efforts.

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A comparison of two different cryoprotectants on post-thaw sperm parameters and fertilization success in tiger salamanders (Ambystoma tigrinum)

Cited by 2 publications

References 0 publications

Oral administration of GnRH via a cricket vehicle stimulates spermiation in tiger salamanders (Ambystoma tigrinum)

Oral administration of GnRH via a cricket vehicle stimulates spermiation in tiger salamanders (Ambystoma tigrinum)

Oral administration of GnRH via a cricket vehicle stimulates spermiation in tiger salamanders (Ambystoma tigrinum)

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