A Comparison of Various Growth Parameters of Cell Suspension Cultures to Determine Phytotoxicity of Xenobiotics

Davis, David W.; Stolzenberg, Rosa L.; Dusky, Joan A.

doi:10.1017/s0043174500058872

Cited by 12 publications

(3 citation statements)

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“…Also, suspension cells are much less fragile than protoplasts isolated from plant tissues and can be manipulated more easily during uptake experiments. Thus, we believe suspension- propano-ate} was influenced more by temperature than metriflufen rate or relative humidity (RH) (11).…”

Section: Discussionmentioning

confidence: 99%

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Glyphosate Uptake by Suspension-Cultured Potato (Solanum tuberosumandS. brevidens) Cells

Burton

Balke

1988

Weed sci.

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Characteristics of glyphosate [N-(phosphonomethyl)glycine] uptake for as long as 3 days by plant cells were studied using suspension-cultured potato (Solanum tuberosumL. cv. ‘Red Pontiac’ andS. brevidensPhil.) cells. An experimental system was developed wherein uptake was assayed in a reduced salt medium that minimized cell growth yet maintained cell viability. Glyphosate uptake was nonlinear over time; an immediate rapid uptake phase was followed by uptake at a decreased rate that continued through 72 h. Glyphosate uptake was the same in both an experimental uptake medium (reduced salt and sucrose, no hormones) and in the complete growth medium used for routine cell growth. The pattern of glyphosate uptake was similar with both Red Pontiac andS. brevidenscells, though uptake was greater in Red Pontiac cells. Glyphosate uptake was not inhibited by 1 mM CaSO4, MgSO4, MnSO4, or ZnSO4. Rather, uptake was inhibited by the absence of salts at 8 h, and ZnSO4and MnSO4increased uptake. Similarly, increasing concentrations (0 to 5 mM) of CaSO4and CaCl2did not inhibit uptake, whereas the absence of either salt of Ca2+decreased uptake. Increasing concentrations of (NH4)2SO4(0 to 150 mM) did not affect glyphosate uptake, but (NH4)2SO4at 50 mM and above was toxic to the cells.

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Section: Discussionmentioning

confidence: 99%

“…The cell aliquot was returned to the original flask. This method allowed for repeated measurement from the same flask without changes in medium or cell volume (11). Cell viability was determined by fluorescein diacetate (FDA) (21, 41) which accumulates within viable, intact cells.…”

Section: Methodsmentioning

confidence: 99%

Glyphosate Uptake by Suspension-Cultured Potato (Solanum tuberosumandS. brevidens) Cells

Burton

Balke

1988

Weed sci.

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“…1. However, the change of pH in other plant cell cultures did not show consistent correlation with the cell growth, for example, parsley and soybean cell lines (Davis et al 1984;Halbrock & Kuhlen 1974), suggesting that the question is more complicated. Under these experimental conditions, the maximum specific growth rate determined by cell weight measurement was found to be 0.32 day -1.…”

Section: Resultsmentioning

confidence: 99%

Monitoring of haploid maize cell suspension culture conditions in bioreactors

Kevács¹,

László²,

Rajkai

et al. 1995

Plant Cell Tiss Organ Cult

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Maize (Zea mays L.) haploid cells were cultivated in a 1500 ml aerated and stirred batch bioreactor using modified BM medium. Cell growth was highly affected by pH and dissolved oxygen, and we observed two fairly distinct growth phases. During the first two days after inoculation at pH 5.8, oxygen consumption was high and the cells lowered the pH to a value around 4.3. After this period the pH stabilized at 4.5 and the dissolved oxygen reached a steady level. Decreasing dissolved oxygen concentration leads to lower growth rate and to higher pH. Both events mean stress conditions for the cell culture and probably result in increased genetic variability, and the loss of regeneration capacity. The stress condition during the adaptation phase can be eliminated by decreasing the pH of the medium to 4.7 before inoculation and by keeping dissolved oxygen above 40%. These conditions provide prolonged exponential growth dynamics and the cell suspensions could be the basis of large scale cultures also.Abbreviations: 2,4-D-2,4-dichlorophenoxyacetitc acid; NAA-naphthalene acetic acid

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