A Complement-Fixing Antigen for Herpes Simplex Derived From Chick-Embryo Tissue Cultures1

Schmidt, Nathalie J.; Lennette, Edwin H.; Shon, Carol W.

doi:10.1093/oxfordjournals.aje.a120134

Cited by 13 publications

(6 citation statements)

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“…IMMUNITY on August 5, 2020 by guest http://iai.asm.org/ Downloaded from active in CF and indirect hemagglutination (13) tests but contained no virions or capsids. These CF antigens are probably the same "soluble" antigens as previously described by others in fluids of disrupted VR # 3-infected cell cultures (12). However, they do not appear to be virion precursors because they failed to react in CF with experimental antiserum to partially purified enveloped or deenveloped particles.…”

Section: Vol 5 1972supporting

confidence: 69%

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Complement-Fixing Antigens of Herpes Simplex Virus Types 1 and 2: Reactivity of Capsid, Envelope, and Soluble Antigens

1972

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Capsid, envelope, and nonvirion-associated soluble components of type I and type 2 herpes simplex virus (HSV) were obtained from infected monolayer cell cultures and used as complement fixation (CF) antigens. Capsids were prepared by treatment of cells with the nonionic detergent Nonidet-P40, envelope material by treatment of virions with ether and high pH, and soluble components were obtained from culture fluids of untreated cells. Serological studies with experimental anti-herpesvirus sera indicate that these serotypes share cross-reacting envelope, capsid, and soluble antigens with each other and with herpesvirus B but not with varicella virus. In addition, animals immunized with crude HSV preparations contain high levels of CF antibody (1: 32 to 1: 64) to soluble antigens, whereas sera from humans who have experienced natural infection contain low levels of antibody (<1:8) to this antigen. Further testing with reference, capsid, and envelope antigens indicates that antibody levels to reference and capsid antigens are about the same in sera from healthy humans, whereas antibody to the envelope is decidedly lower in these sera. Herpes convalescent-phase sera contain higher levels of antibody to reference and envelope antigens than to capsid antigen. 248 on August 5, 2020 by guest http://iai.asm.org/ Downloaded from

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Section: Vol 5 1972supporting

confidence: 69%

“…MATERIALS AND METHODS Virus. The VR # 3 strain of type 1 (12) and the MS strain of type 2 (4) HSV were used to prepare HSV antigens and antisera. HVB was obtained from Robert Hull, Eli Lilly & Co., Indianapolis, Ind.…”

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confidence: 99%

Complement-Fixing Antigens of Herpes Simplex Virus Types 1 and 2: Reactivity of Capsid, Envelope, and Soluble Antigens

1972

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“…Questionable isolates or isolates from "atypical" disease were identified by fluorescent-antibody staining with rabbit anti-HSV antisera. Documentation of serological status was by assay of CF antibody to HSV (13). Blood samples were drawn from donors within 2 to 6 weeks after HSV recurrence unless otherwise indicated.…”

mentioning

confidence: 99%

Lymphocyte transformation and interferon production in human mononuclear cell microcultures for assay of cellular immunity to herpes simplex virus

Haahr¹,

Rasmussen²,

Merigan³

1976

Infect Immun

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Interferon production and transformation in respowse to herpes simplex virus antigen were studied in microcultures of human mononuclear cells. Mononuclear cells consisting of monocytes and both T and B lymphocytes were purified by Ficoll-Hypaque gradients. Lymphocytes, predominantly T with 5% B, were obtained by passage of buffy-coat cells through nylon fiber columns. For some experiments, autochthonous macrophages and column-purified lymphocytes were stimulated with herpesvirus antigen. The effect of specific antibody and cell concentration on reactivity is described. Crude and purified antigens were compared as cell culture stimulants. Significant differences in transformation and interferon were observed between donors with a history of herpes labialis and donors with no detectable antibody, both in cultures prepared by Ficoll-Hypaque gradients and by column purification of lymphocytes. Cultures from seronegative donors prepared by Ficoll-Hypaque gradients produced interferon but did not transform when stimulated by herpes simplex antigen. "Immune" interferon production, that is, type II as opposed to type I, occurred only with autochthonous macrophage and columm-purified lymphocyte cultures. Interferon produced by Ficoll-Hypaque-purified mononuclear cultures was type I, and its production was unrelated to immune status. Similarly, column-purified lymphocytes responded to herpes simplex virus antigen with type I interferon if obtained from a seropositive donor.

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“…The effect of the composition of the medium on CF antigenicity varies with viruses --a serum-free medium did not reduce CF antigenicity of EHV 1 in these studies, or for respiratory syncytial virus [JAcoBS, (13)], whereas SCHMIDT et al, (23) found the medium composition to be important for the CF antigenicity of herpes simplex virus. Antigen produced using a medium free of serum lacked the hemagglutinating activity of some heterologous serums on sheep RBC settling patterns.…”

Section: Discussionmentioning

confidence: 83%

Studies on equine herpesviruses

Bagust

Pascoe

1972

Archiv f Virusforschung

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A complement fixation (CF) antigen for equine herpes-1 (rhinopneumonitis) virus (EHV 1) of cell culture origin was studied, and an optimum method of preparation in a minimum volume of serum-flee overlay fluid developed. Many chemical and physical treatments including fluorocarbon extraction, tweenether treatment, Carbowax concentration or ultracentrifugation (25,000 g) did not further improve the quality of this antigen. Inactivation (56~ for 30 minutes) of equine serum did not affect the CF antibody titre, but removed low levels of anti-complementary activity. The use of a diluent of low ionic strength (0.1 M) did not increase titres in the EHV 1-equine antibody CF system. This EHV 1 CF antigen of cell culture origin was used in a microtitre system and detected the development of antibodies in all of six young horses in Queensland following natural EHV 1 respiratory infection. Maximum serum titres (1/128) were reached 1--2 weeks after the onset of acute respiratory disease, then CF titres declined steadily but were still detectable after 12 months.Serum titres of 1/16--1/32 or higher indicated EHV1 infection to have occurred within the previous 3--6 months.

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A Complement-Fixing Antigen for Herpes Simplex Derived From Chick-Embryo Tissue Cultures1

Cited by 13 publications

References 0 publications

Complement-Fixing Antigens of Herpes Simplex Virus Types 1 and 2: Reactivity of Capsid, Envelope, and Soluble Antigens

Complement-Fixing Antigens of Herpes Simplex Virus Types 1 and 2: Reactivity of Capsid, Envelope, and Soluble Antigens

Lymphocyte transformation and interferon production in human mononuclear cell microcultures for assay of cellular immunity to herpes simplex virus

Studies on equine herpesviruses

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