A completely noninvasive method of dissolved oxygen monitoring in disposable small‐scale cell culture vessels based on diffusion through permeable vessel walls

Gupta, Priyanka A.; Ge, Xudong; Kostov, Yordan; Rao, Govind

doi:10.1002/btpr.1838

Cited by 14 publications

(8 citation statements)

References 22 publications

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“…Media components such as vitamins may also be deteriorated if exposed to the illuminating light for a long time. We have reported a completely noninvasive approach to monitoring the DO and DCO 2 in disposable small‐scale cell culture vessels based on the diffusion through the permeable vessel walls (Gupta et al, ). This approach is noninvasive and applicable to monitoring culture processes of extremely sensitive cells.…”

Section: Resultsmentioning

confidence: 99%

A unique noninvasive approach to monitoring dissolved O₂ and CO₂ in cell culture

Chatterjee

Uplekar

et al. 2014

Biotech & Bioengineering

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Although online monitoring of dissolved oxygen (DO) and carbon dioxide (DCO2 ) is highly desirable in bioprocesses, small-scale bioreactors are usually not monitored due to the lack of suitable sensors. Traditional electrochemical sensors are usually not used because they are bulky and invasive. Disposable optical sensors are small and only partially invasive, but there are concerns regarding the toxicity of the patch and the phototoxicity of the illuminating light. Here we present a novel, noninvasive, rate-based technique for monitoring DO and DCO2 in cell cultures. A silicone sampling loop which allowed the diffusion of O2 and CO2 through its wall was inserted inside a bioreactor, and then flushed with N2 until the CO2 and O2 inside the loop were completely removed. The gas inside the loop was then allowed to recirculate through gas impermeable tubing to the O2 and CO2 sensors. We have shown that by measuring the initial diffusion rate we were able to determine the partial pressures of the two gases in the culture. The technique could be readily automated and measurements could be made in minutes. It was tested in demonstration experiments by growing murine hybridoma cells in a T-flask and a spinner-flask at 37°C. The results were comparable to those measured with commercially available fluorescence-based patch sensors. These results show that the rate-based method is an effective way to monitor small-scale cell cultures. This measurement mechanism can be easily built into disposable cell culture vessels for facile use.

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Section: Resultsmentioning

confidence: 99%

A unique noninvasive approach to monitoring dissolved O₂ and CO₂ in cell culture

Chatterjee

Uplekar

et al. 2014

Biotech & Bioengineering

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“…Doran [92] explains in great details all the steps involved in calculating the dO2 level in a cell culture system namely fermenters, bioreactors and shake flasks. However, monitoring dO2 in small scale plastic cell culture flasks is less common but methods have been published on how to perform it [93,94].…”

Section: Dissolved Oxygen Levels In Hmsc Culturementioning

confidence: 99%

The Role of Dissolved Oxygen Levels on Human Mesenchymal Stem Cell Culture Success, Regulatory Compliance, and Therapeutic Potential

Bahsoun

Coopman

Forsyth

et al. 2018

Stem Cells and Development

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Most cells in the human body, including human mesenchymal stem cells (hMSCs), have evolved to survive and function in a low physiological oxygen (O) environment. Investigators have become increasingly aware of the effects of O levels on hMSC biology and culture and are mimicking the natural niche of these cells in vitro to improve cell culture yields. This presents many challenges in relation to hMSC identity and function and in the maintenance of a controlled O environment for cell culture. The aim of this review was to discuss an "hMSC checklist" as a guide to establishing which identity and potency assays to implement when studying hMSCs. The checklist includes markers, differentiation potential, proliferation and growth, attachment and migration, genomic stability, and paracrine activity. Evidence drawn from the current literature demonstrates that low O environments could improve most "hMSC checklist" attributes. However, there are substantial inconsistencies around both the terminology and the equipment used in low O studies. Therefore, "hypoxia" as a term and as a culture condition is discussed. The biology of short-term (acute) versus long-term (chronic) hypoxia is considered, and a nascent hypothesis to explain the behavior of hMSCs in long-term hypoxia is presented. It is hoped that by establishing an ongoing discourse and driving toward a regulatory recognizable "hMSC checklist," we may be better able to provide the patient population with safe and efficacious regenerative treatments.

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“…Another very interesting system for noninvasive measurement of the partial pressure of oxygen is shown by Gupta et al. . They demonstrated the measurement of oxygen concentration through the SUB wall itself.…”

Section: Interface Category 3: Noninvasive Interfacementioning

confidence: 99%

Sensors for disposable bioreactors

Busse

Biechele

Vries

et al. 2017

Engineering in Life Sciences

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Modern bioprocess monitoring demands sensors that provide on-line information about the process state. In particular, sensors for monitoring bioprocesses carried out in single-use bioreactors are needed because disposable systems are becoming increasingly important for biotechnological applications. Requirements for the sensors used in these single-use bioreactors are different than those used in classical reusable bioreactors. For example, long lifetime or resistance to steam and cleaning procedures are less crucial factors, while a requirement of sensors for disposable bioreactors is a cost that is reasonable on a per-use basis. Here, we present an overview of current and emerging sensors for single-use bioreactors, organized by the type of interface of the sensor systems to the bioreactor. A major focus is on non-invasive, in-situ sensors that are based on electromagnetic, semiconducting, optical, or ultrasonic measurements. In addition, new technologies like radio-frequency identification sensors or free-floating sensor spheres are presented. Notably, at this time there is no standard interface between single-use bioreactors and the sensors discussed here.In the future, manufacturers should address this shortcoming to promote single-use bioprocess monitoring and control.

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A completely noninvasive method of dissolved oxygen monitoring in disposable small‐scale cell culture vessels based on diffusion through permeable vessel walls

Cited by 14 publications

References 22 publications

A unique noninvasive approach to monitoring dissolved O₂ and CO₂ in cell culture

A unique noninvasive approach to monitoring dissolved O₂ and CO₂ in cell culture

The Role of Dissolved Oxygen Levels on Human Mesenchymal Stem Cell Culture Success, Regulatory Compliance, and Therapeutic Potential

Sensors for disposable bioreactors

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A completely noninvasive method of dissolved oxygen monitoring in disposable small‐scale cell culture vessels based on diffusion through permeable vessel walls

Cited by 14 publications

References 22 publications

A unique noninvasive approach to monitoring dissolved O2 and CO2 in cell culture

A unique noninvasive approach to monitoring dissolved O2 and CO2 in cell culture

The Role of Dissolved Oxygen Levels on Human Mesenchymal Stem Cell Culture Success, Regulatory Compliance, and Therapeutic Potential

Sensors for disposable bioreactors

Contact Info

Product

Resources

About

A unique noninvasive approach to monitoring dissolved O₂ and CO₂ in cell culture

A unique noninvasive approach to monitoring dissolved O₂ and CO₂ in cell culture